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Growth medium

About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.


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Journal ArticleDOI
TL;DR: The production of 7-methyljuglone in D. c apensis under in vivo conditions by salicylic acid and jasmonic acid elicitation and by applying different strengths of media as well as different levels of total inorganic nitrogen (N) and ratios of nitrate to ammonium to a plant tissue culture medium is reported.

24 citations

Journal ArticleDOI
TL;DR: The production of L-asparaginase by two mutants ofSerratia marcescens grown on 14 different media was studied and glucose was the best carbon source under aerobic conditions and the enzyme content increased when L- asparagine was present in the growth medium.
Abstract: The production of L-asparaginase by two mutants of Serratia marce- scens grown on 14 different media was studied. The enzyme content increased from trace levels to 2·4 international units per ml when the organisms were grown in glycerol-peptone yeast extract medium. Glucose was the best carbon source under aerobic conditions. The enzyme content increased when L-asparagine was present in the growth medium.

24 citations

Journal ArticleDOI
TL;DR: In this paper, the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM.
Abstract: Glucose is a major energy source consumed by proliferating mammalian cells. Therefore, in general, proliferating cells have the preference of high glucose contents in extracellular environment. Here, we showed that high glucose concentrations impede the proliferation of satellite cells, which are muscle-specific stem cells, under adherent culture conditions. We found that the proliferation activity of satellite cells was higher in glucose-free DMEM growth medium (low-glucose medium with a glucose concentration of 2 mM) than in standard glucose DMEM (high-glucose medium with a glucose concentration of 19 mM). Satellite cells cultured in the high-glucose medium showed a decreased population of reserve cells, identified by staining for Pax7 expression, suggesting that glucose concentration affects cell fate determination. In conclusion, glucose is a factor that decides the cell fate of skeletal muscle-specific stem cells. Due to this unique feature of satellite cells, hyperglycemia may negatively affect the regenerative capability of skeletal muscle myofibers and thus facilitate sarcopenia.

24 citations

Journal ArticleDOI
TL;DR: In a growth medium containing peptone, yeast extract, and serum, yields obtained with pyruvate were comparable with those for glucose, suggesting that pyruVate is able to act as an energy source.
Abstract: Washed cell suspensions of Mycoplasma mycoides oxidized glucose and pyruvate. In a growth medium containing peptone, yeast extract, and serum, yields obtained with pyruvate were comparable with those for glucose, suggesting that pyruvate is able to act as an energy source. Mycoplasma agalactiae and M. F38 failed to oxidize glucose and glucose had no effect on growth yield. Pyruvate, however, was oxidized and markedly enhanced growth. Lactate was also oxidized by a number of mycoplasma strains but did not increase growth yield in statically incubated cultures.

24 citations

Journal ArticleDOI
TL;DR: The results indicate that human fetal cardiac muscle cells proliferate in vitro and can maintain a phenotype characteristic of fetal myocytes after multiple subcultivations followed by serum withdrawal, and after subcultivation in growth medium, some myocytes modulate their phenotype into one in which detectable levels of cardiac contractile proteins are expressed only after mitogen withdrawal.
Abstract: Previous work has suggested that subcultivated human fetal heart muscle cell cultures contain immature cardiac muscle cells capable only of limited differentiation after mitogen withdrawal. We studied several human fetal heart cultures (14-15 wk gestation) at several passage levels using immunocytochemistry, autoradiography, and Northern blot analysis. Characteristics in high-mitogen (growth) medium were compared with those after serum withdrawal. Cultured cells from one heart, expanded through 2 passages in growth medium, did not beat; however, 75% of cells did beat after subsequent culture for 24 days in low-serum (differentiation) medium containing insulin. In confluent cultures after 1 passage, there was no detectable difference in the number of cardiac myocytes present in growth medium compared with that 7 days after serum withdrawal. After 4 passages, however, serum withdrawal increased the number of cells expressing immunoreactive sarcomeric myosin heavy chain by 100-fold; expression of immunoreactive sarcomeric actin and alpha-cardiac actin mRNA also increased in the same cultures. Similar results were obtained in cultures kept in differentiation medium for 20 days before passage and expansion in growth medium. Using isopycnic centrifugation, a high-density cell fraction was isolated which contained no immunostained myocytes in growth medium but numerous myocytes after serum withdrawal. Combined immunocytochemistry/autoradiography showed that myocytes synthesize DNA in growth medium and in serum-free medium containing fibroblast growth factor, but not in serum-free medium alone. The results indicate that a) human fetal cardiac muscle cells proliferate in vitro and can maintain a phenotype characteristic of fetal myocytes after multiple subcultivations followed by serum withdrawal; b) after subcultivation in growth medium, some myocytes modulate their phenotype into one in which detectable levels of cardiac contractile proteins are expressed only after mitogen withdrawal, and c) the phenotype attained after serum withdrawal is in part dependent on passage level. Cultured human fetal myocardial cells may provide a useful experimental system for the study of human cardiac muscle cell biology.

24 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20233
20226
202126
202032
201926
201829