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Growth medium

About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.


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Book ChapterDOI
18 Aug 2020
TL;DR: In this paper, the optimal and maximal salt concentrations for growth of Deleya halophila are shifted up by increasing temperature in the range of 22 to 42 C. Though halophilic archaebacteria are usually grown at 37 C, their optimal temperature for growth is probably higher; values up to 50 C for this have been reported.
Abstract: For practical purposes, the growth medium should be defined as “rich”, or more accurately, the medium that supports the most rapid growth under optimal salt concentrations. To return to such techniques, yeast extract seems to serve well for the growth of most halophilic archaebacteria, but all constituents of complex media do not. Both the optimal and the maximal salt concentrations for growth of Deleya halophila are shifted up by increasing temperature in the range of 22 to 42 C. Richer nutritional conditions can also widen the salt range of growth. Though halophilic archaebacteria are usually grown at 37 C, their optimal temperature for growth is probably higher; values up to 50 C for this have been reported. Interestingly, growth at, or even exposure to, higher salt concentrations may be accompanied by an adaptation in active transport systems of several bacterial species, so that cells can take in solutes at higher external salt concentrations than before.

22 citations

Journal ArticleDOI
TL;DR: The formation of the bisphosphatidic acids may be specifically linked to the autolysis of the phospholipids of the cellular membranes and the formation of triglycerides associated with this process.

22 citations

Journal ArticleDOI
TL;DR: The correcting effect of high ionic strengths of the growth medium on the morphology of this mutant seems to be mostly due to the increased effectiveness of L-glutamate or L- glutamine in the presence of high concentrations of salts.
Abstract: SUMMARY The morphological mutant rod-4 derived from Bacillus subtilis 168 trp can be changed from a round form to a rod by the addition to the growth medium of sufficient acid-hydrolysate of casein. The hydrolysate can be replaced by a mixture of amino acids, and the only individual amino acids giving similar results are L-glutamic acid, L-proline, L-arginine and L-ornithine. Since the lag in the action of L-glutamate was less than for the other amino acids, this amino acid is likely to be responsible for the effect of the mixture. Experiments with the L-glutamine analogue, γ-L-glutamylhydrazide, strongly suggest that L-glutamine is the active metabolite rather than the amino acid itself. The correcting effect of high ionic strengths of the growth medium on the morphology of this mutant seems to be mostly due to the increased effectiveness of L-glutamate or L-glutamine in the presence of high concentrations of salts.

22 citations

Journal ArticleDOI
TL;DR: In this paper, the effect of silver thiosulfate (STS) in reducing ethylene-induced culture abnormalities during minimal growth conservation of microplants was studied in seven potato (Solanum tuberosum L.) genotypes.
Abstract: Efficacy of silver thiosulfate (STS) in reducing ethylene-induced culture abnormalities during minimal growth conservation of microplants was studied in seven potato (Solanum tuberosum L.) genotypes. Different concentrations of STS (0, 1.5, 3.0, 4.5, 6.0, 7.5 and 9.0 μg ml–1) were tested in minimal growth medium based on MS medium supplemented with 20 g l–1 mannitol and 40 g l–1 sucrose. STS improved the microplant growth and reduced the culture abnormalities during prolonged maintenance of potato shoot cultures in vitro. The beneficial effect of STS was most prominent for number of green leaves per microplant and leaf senescence. After 16 months of storage, desirable microplant growth was observed in cultures conserved in medium containing 6.0–9.0 μg ml–1 STS. The profile of the peroxidase isozymes of conserved cultures did not show any apparent genetic variation due to the presence of STS in the conservation medium.

22 citations

Journal ArticleDOI
TL;DR: Primary cultures of liver cells and kidney cells of lake trout Salvelinus namaycush were initiated in a hormone-supplemented, serum-reduced medium in plastic dishes and cells plated on fibronectin-coated plastic attached with high efficiency, in contrast to cells cultured on uncoated or polymer- coated plastic.
Abstract: Primary cultures of liver cells and kidney cells of lake trout Salvelinus namaycush were initiated in a hormone-supplemented, serum-reduced medium in plastic dishes. Cells plated on fibronectin-coated plastic attached with high efficiency (87–93%), in contrast to cells cultured on uncoated or polymer-coated plastic (< 8–52%). Primary cultures were obtained from young lake trout by trypsin-EDTA dissociation at 10–15°C for 40 min; the primary cultures were maintained in a growth medium (either medium 199 or Leibovitz's L-15 medium) supplemented with 2–3% fetal bovine serum, epidermal growth factor, bovine serum albumin, cholera toxin, insulin, transferrin, and selenium. Lake trout liver and kidney cultures currently contain both epithelioid and fibroblastic cells. The cells, cultivated at 18–20°C from primary culture, have been subcultured 32 times over a period of 6 months.

22 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20233
20226
202126
202032
201926
201829