Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: The results suggest that the presence of oxygen in the growth medium decreases the content of fructose-2,6-P 2 through inactivation ofructose- 6-P,2-kinase.
17 citations
TL;DR: A correlation has been made between radioresistance and ability to initiate rapid new protein synthesis in several strains, and this capacity to synthesize protein correlates with the types of ribosomes present in the cell.
Abstract: A number of strains of E. coli develop radioresistance in the stationary phase when glucose is present in a natural growth medium. Cells grown in such a medium have a large excess of macromolecules per cell, compared with cells grown to the same phase of the growth cycle in the same medium without this carbon source. The amounts of the various classes of ribosomes found in extracts of resistant and sensitive cells are different; the excess RNA in resistant cells is accounted for largely by the amount of 30S and 50S ribosomal particles. A correlation has been made between radioresistance and ability to initiate rapid new protein synthesis. In several strains this capacity to synthesize protein correlates with the types of ribosomes present in the cell. We have not been able to quantitate in any precise manner many of the apparent correlations demonstrated here. Such data can result only from better understanding of the multicomponent systems involved in these complex syntheses.
17 citations
TL;DR: The results indicate that arthrobacters exhibit characteristic responses to osmotic stress and suggest these bacteria may contain novel osmoprotective compounds.
Abstract: C.E. DEUTCH AND G.S. PERERA. 1992. Arthrobacter globiformis was grown in a semi-defined liquid medium containing added solutes to determine the effects of osmotic stress on its reproduction and cell morphology. There was a progressive reduction in the specific growth rate during exponential phase as the concentration of NaCl was increased, although the final yields of the cultures during stationary phase were not affected. Clusters of branching myceloid cells rather than the typical bacillary forms predominated during exponential phase. These myceloids did not undergo complete septation and persisted into stationary phase. Similar responses were observed with potassium sulphate as the exogenous solute but less dramatic morphological effects were found with added polyethylene glycol or sucrose. The myceloids formed in response to osmotic stress could not be disrupted mechanically but were more sensitive than normal cells to lysozyme, particularly during stationary phase. Addition of osmoprotective compounds such as proline, glutamate, glycine betaine, or trehalose to the growth medium did not significantly relieve the effects of osmotic stress on growth rate or morphology. A. simplex also formed myceloid cells during osmotic stress but A. crystallopoietes did not. These results indicate that arthrobacters exhibit characteristic responses to osmotic stress and suggest these bacteria may contain novel osmoprotective compounds.
17 citations
TL;DR: The ability of a sugar carbon source to evoke catabolite repression appears to be unrelated to its suitability as a substrate for the sugar, but nonetheless the conversion to a phosphorylated derivative of the sugar seems essential.
17 citations
TL;DR: It is demonstrated that the two-phase assay system (FC43-growth medium interphase) may be superior to soft agar for monitoring the anchorage-independent growth of cells because of the ease of cell plating, the ability to recover cells and secreted products from the upper aqueous phase, and the shorter growth period required to complete the assay.
Abstract: A high density, purified, nontoxic solvent, heptacosafluorotributylamine (FC43), was successfully used as a culture surface for growing several normal and oncogene-transformed cell lines under anchorage-independent conditions. Normal rat kidney (NRK) fibroblasts and the normal mammary epithelial cell lines NMuMG and A1, clone N4, of murine and human origin, respectively, failed to grow at a FC43 growth medium interphase or in soft agar in the absence of transforming growth factor alpha (TGFα) and transforming growth factor beta (TGFβ). However, NRK fibroblasts transformed with the Kirstenras viral oncogene (K-NRK) or NMuMG cells transformed with a point-mutated c-Harvey-ras proto-oncogene or polyoma middle T-transforming gene (NMuMG-rasH and NMuMG-pyt, respectively) exhibited rapid growth and formed large colonies when cultured on an FC43-medium interphase. In addition, NRK cells treated with TGFα and TGFβ and K-NRK cells grown on FC43 exhibited a sensitivity to the growth inhibitory effects of 4-cis-L-hydroxyproline comparable to that observed for the same cells grown in soft agar. These results demonstrated that the two-phase assay system (FC43-growth medium interphase) may be superior to soft agar for monitoring the anchorage-independent growth of cells because of the ease of cell plating, the ability to recover cells and secreted products from the upper aqueous phase, and the shorter growth period required to complete the assay (3–4 days).
17 citations