Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: The requirement and the effects of inhibitors suggest that the protein of catalase is synthesized de novo, although aeration causes this induced synthesis, energy for the process can be derived either from air or light.
78 citations
TL;DR: A strong positive correlation between Artemia performance and the yeast cell wall chitin and glucan content was obtained, while the mannoprotein content was negatively correlated, and yeast cell viability and the method used to kill/sterilize the cells are important parameters influencing nauplii performance.
77 citations
TL;DR: The ability of various biological materials to promote growth of Bifidobacterium Iongum (ATCC 15708) was tested using Bacto B12 assay medium and yeast extract, α- lactalbumin and β-lactoglobulin were the best growth promoters.
Abstract: The ability of various biological materials to promote growth of Bifidobacterium Iongum (ATCC 15708) was tested using Bacto B12 assay medium (Difco Corp.). Supplements included yeast extract, beef extract, malt extract, α-lactalbumin, β-lactoglobulin, trypticase soy broth, phytone-peptone and unknown factors from Escherichia coli spent broth. Growth of B. longum 15708 was monitored by measuring turbidity and pH. Yeast extract, α-lactalbumin and β-lactoglobulin were the best growth promoters. Growth in the presence of E. coli spent broth was maximal; however, fresh enzymatically hydrolyzed E. coli broth was as effective. Beef extract and trypticase soy broth were effective to some extent. Malt extract and phytone-peptone did not significantly enhance growth. All materials lost growth promoting activity when their disulfide bonds were reduced-alkylated.
77 citations
TL;DR: E. coli subjected to osmotic shock have difficulty in adapting to synthetic growth media when previously grown on a richer, semidefined medium, and active transport of l-leucine is impaired, confirming a report in the literature.
77 citations
TL;DR: Results indicate that antibody accumulation in the growth medium is genuinely caused by rhizosecretion and not cell damage, and may be a viable alternative to agricultural production or cell culture for the generation of monoclonal antibodies in transgenic plants.
Abstract: The secretion of a functional, full-length monoclonal antibody complex from transgenic Nicotiana tabacum roots has been demonstrated. Initially, seeds were germinated on nitrocellulose membranes and antibody secretion detected from the developing roots. Plants were then established in hydroponic culture and secretion into the growth medium measured over 25 days. Western blotting indicated that full-length antibody was present in the medium along with other fragments. Secreted antibody was shown to be functional by binding to antigen in ELISA studies. In contrast, no antibody could be detected from transgenic Nicotiana in which the same antibody was expressed as a membrane protein in the plasmalemma. These results indicate that antibody accumulation in the growth medium is genuinely caused by rhizosecretion and not cell damage. Addition of gelatin to plant growth medium markedly increased levels of antibody accumulation. The mean antibody yield per plant was calculated to be 11.7 μg per gram root dry weight per day. Rhizosecretion may be a viable alternative to agricultural production or cell culture for the generation of monoclonal antibodies in transgenic plants. It may also give rise to novel applications for antibodies expressed in plants such as removal or neutralisation of environmental pollutants and attenuation of pathogens which infect the plant via the rhizosphere.
77 citations