Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: A growth medium was developed that included ions that were more easily assimilated by Chlorella vulgaris, such as ammonium one (NH(4)(+)), which improved recycling performance and accumulation of ions over time.
67 citations
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TL;DR: The activities of two glutamate dehydrogenases, one requiring NAD and the other specific for NADP, and of aspartate and alanine amino transferases varied during growth as a function of the stage of the life cycle and of the growth medium, finding no clear correlation between the activities of these enzymes and conidiation of this fungus.
Abstract: SUMMARY: Asexual sporulation of Aspergillus niger occurred in submerged culture in a liquid minimal medium without added nitrogen, in low ammonium-N concentrations, and in a wide range of nitrate-N concentrations. Ammonium salts containing more than 48 mg. atom N/l. were inhibitory to conidiation. Most amino acids overcame the ammonium inhibition of conidiation when added to an ammonium nitrate medium. Glyoxylate and several intermediates of the tricarboxylic acid (TCA) cycle also promoted conidiation in the presence of ammonium. Changes in the medium of conidiating and non-conidiating cultures were examined with respect to nitrogen and glucose concentrations, dry weight and pH value. The activities of two glutamate dehydrogenases, one requiring NAD and the other specific for NADP, and of aspartate and alanine amino transferases varied during growth as a function of the stage of the life cycle and of the growth medium. There was no clear correlation between the activities of these enzymes and conidiation of this fungus.
66 citations
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TL;DR: Natural abundance 13C nuclear magnetic resonance spectroscopy identified the disaccharide trehalose as the major organic osmolyte synthesized by Escherichia coli grown in continuous culture under nitrogen limitation in the presence of 0.5 M-NaCl.
Abstract: Summary: Natural abundance 13C nuclear magnetic resonance spectroscopy identified the disaccharide trehalose as the major organic osmolyte synthesized by Escherichia coli grown in continuous culture under nitrogen limitation in the presence of 0·5 m-NaCl. Trehalose accumulation was dependent on both the growth phase of the culture and the osmolality of the growth medium, but independent of the solute used to increase the osmolality as long as the solute was non-penetrant. The penetrant solute glycerol did not induce trehalose synthesis indicating that the loss of cell turgor rather than increasing medium osmolality per se was the mechanism stimulating trehalose synthesis. Under conditions of either carbon or nitrogen limitation osmoadaptation was distinctly biphasic. The initial response consisted of a rapid (within 30 min) accumulation of K+ and a concurrent synthesis of the amino acid glutamate; trehalose synthesis occurred during the second slower phase of osmoadaption. Chloramphenicol severely inhibited trehalose accumulation indicating that the enzyme(s) involved in trehalose synthesis were inducible.
66 citations
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TL;DR: Growth and enzyme development in cell cultures of fetal rat brain were influenced by type of growth medium, cell density, and age of fetal tissue source, and some results suggested division of choline acetyltransferase producing cells.
66 citations
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TL;DR: Cyanogenesis by growing cultures of Chromobacterium violaceum was stimulated by the inclusion of glycine and methionine in the growth medium, and addition of chloramphenicol to high and low cyanide-evolving cultures towards the end of exponential growth had a profound effect on the medium cyanide concentrations.
Abstract: Cyanogenesis by growing cultures of Chromobacterium violaceum was stimulated by the inclusion of glycine and methionine in the growth medium. Increases in the ferrous ion and phosphate concentrations of the growth medium stimulated cyanide production. Chromobacterium violaceum possesses a number of cyanide-utilizing enzymes: β-cyanoalanine synthase, γ-cyano-α-aminobutyric acid synthase and rhodanese. Studies on the activities of these enzymes in cell-free extracts of cultures growing under both high and low cyanide-evolving conditions are presented. Addition of chloramphenicol to high and low cyanide-evolving cultures towards the end of exponential growth had a profound effect on the medium cyanide concentrations. These observations are shown to have been caused by chloramphenicol blocking the induction of the cyanide-utilizing enzymes.
66 citations