Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: Several cultures of bacteria, isolated from the rumen, that were able to utilize plant cell wall structural polysaccharides were grown on a range of carbohydrate substrates and the activities of the principalpolysaccharide-degrading enzymes determined.
Abstract: Several cultures of bacteria, isolated from the rumen, that were able to utilize plant cell wall structural polysaccharides were grown on a range of carbohydrate substrates and the activities of the principal polysaccharide-degrading enzymes determined. The esterase activity was also monitored. The extent of hemicellulose degradation and utilization by the isolates was comparable with that of the hemicellulolytic type strains. Enzyme activities in all of the cultures examined were affected by the carbon source in the growth medium. Many responses were strain specific, although growth on glucose (or cellobiose and maltose to a lesser extent) resulted in reduced activities in most of the organisms examined, whilst polysaccharidic substrates resulted in higher levels of the appropriate polysaccharidase. However, enzyme activity was detectable in some isolates after culture on mono- or disaccharides in the absence of the principal or related polysaccharide substrate.
48 citations
TL;DR: It was found that the phospholipids of secondary chick fibroblasts, labeled with 32 PO 4 or 14 C-choline, were continuously released into the growth medium, and these lipid-protein complexes could be fractionated by gel filtration into two fractions.
48 citations
TL;DR: Cells grown in the mixed nitrogen source had a lower content of total fatty acids with a higher unsaturation degree than cells grown on sole ammonia.
48 citations
TL;DR: Toxin was produced during the late exponential and early stationary phase of growth by the bacterium and, contrary to studies with other toxins, was unaffected by Fe and P concentrations in the growth medium.
Abstract: We studied the production of a toxin inhibitory to both winter wheat (Triticum aestivum L.) root growth andEscherichia coli that was produced by a rhizobacterial pseudomonad. Of several carbon sources tested, the most rapid growth and highest toxin concentrations were obtained with glucose, glycerol, or trehalose. Toxin production was repressed with L-cysteine as the nitrogen source. Toxin was produced during the late exponential and early stationary phase of growth by the bacterium and, contrary to studies with other toxins, was unaffected by Fe and P concentrations in the growth medium. Toxin production by the bacterium was the same at growth temperatures of 25 and 15°C while it produced less at 5°C. If the bacterium was able to grow, it produced toxin. No compound tested induced an increase in toxin production indicating toxin production is constitutive.
48 citations
TL;DR: A period of culture within collagen is required to permit mammary epithelial cells to become responsive for hormone-induced differentiation, and it is possible that during growth within the collagen the cells synthesize and deposit extracellular matrix components important in modulating gene expression.
47 citations