Topic
Growth medium
About: Growth medium is a research topic. Over the lifetime, 1889 publications have been published within this topic receiving 59171 citations. The topic is also known as: culture medium & culture media.
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TL;DR: Microalgae are common in both industrial and scientific cultivation and have various applications such as a health food, fish feed and nutrition supplements for human consumption, as well as for lipid and biodiesel production.
Abstract: Recently, microalgae are common in both industrial and scientific cultivation. There are different fields of application for microalgae includes food, biofuels, fish feed and pharmaceutical products [1]. Recently, various applications were found for Chlorella vulgaris (C. vulgaris) such as a health food, fish feed and nutrition supplements for human consumption, as well as for lipid and biodiesel production. Algae produced its own food by autotrophic nutrition. The food produced is stored as carbohydrates (mainly as starch) and lipid [2,3].
42 citations
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TL;DR: The time at which mycelial development reached a peak and gave way to the production of budding yeast cells was directly proportional to the minimum doubling time of C. albicans in each medium.
Abstract: Five liquid media were tested for their ability to promote filamentation in Candida albicans. Three isolates, including one atypical variant, all developed mycelium in the early stages of growth. The proportion of mycelium produced was highest in the complex media with slightly alkaline pH values (7·5 to 8·6). The time at which mycelial development reached a peak and gave way to the production of budding yeast cells was directly proportional to the minimum doubling time of C. albicans in each medium. The principal effect of the medium was initially to induce filamentation in a greater or lesser number of blastospores.
41 citations
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TL;DR: Pseudomonas fluorescens appears to elicit disparate lead detoxification mechanisms in phosphate-rich and phosphate-deficient media, and the inclusion of lead did not appear to enhance the production of either extracellular proteins or carbohydrates.
Abstract: Pseudomonas fluorescens appears to elicit disparate lead detoxification mechanisms in phosphate-rich and phosphate-deficient media. When grown in the presence of 0.1 mM Pb2+ complexed to citrate, the sole source of carbon, only a slight diminution in cellular yield was observed in the former medium. However, in a phosphate-deficient milieu, lead imposed approximately a 30% reduction in bacterial multiplication. At stationary phase of growth, 72% of the metal was found in the bacterial cells from the phosphate-deficient medium, while that from phosphate-rich broth contained only 12.5% The latter medium was characterised by an insoluble pellet that accounted for 73.5% of the lead. Although no citrate was detected in the phosphate-rich media after 40 h of incubation, only 72% of citrate was consumed even after 70 h of growth in the phosphate-deficient cultures. The inclusion of lead did not appear to enhance the production of either extracellular proteins or carbohydrates.
41 citations
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TL;DR: It is concluded that OsPAP26 performs dual functions in plants: Pi remobilization from senescing to non-senescing leaves; and organic P utilization.
Abstract: During phosphate (Pi) starvation or leaf senescence, the accumulation of intracellular and extracellular purple acid phosphatases (PAPs) increases in plants in order to scavenge organic phosphorus (P). In this study, we demonstrated that a PAP-encoding gene in rice, OsPAP26, is constitutively expressed in all tissues. While the abundance of OsPAP26 transcript is not affected by Pi supply, it is up-regulated during leaf senescence. Furthermore, Pi deprivation and leaf senescence greatly increased the abundance of OsPAP26 protein. Overexpression or RNA interference (RNAi) of OsPAP26 in transgenic rice significantly increased or reduced APase activities, respectively, in leaves, roots and growth medium. Compared with wild-type (WT) plants, Pi concentrations of OsPAP26-overexpressing plants increased in the non-senescing leaves and decreased in the senescing leaves. The increased remobilization of Pi from the senescing leaves to non-senescing leaves in the OsPAP26-overexpressing plants resulted in better growth performance when plants were grown in Pi-depleted condition. In contrast, OsPAP26-RNAi plants retained more Pi in the senescing leaves, and were more sensitive to Pi starvation stress. OsPAP26 was found to localize to the apoplast of rice cells. Western blot analysis of protein extracts from callus growth medium confirmed that OsPAP26 is a secreted PAP. OsPAP26-overexpressing plants were capable of converting more ATP into inorganic Pi in the growth medium, which further supported the potential role of OsPAP26 in utilizing organic P in the rhizosphere. In summary, we concluded that OsPAP26 performs dual functions in plants: Pi remobilization from senescing to non-senescing leaves; and organic P utilization.
41 citations
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TL;DR: For stimulation of PHB accumulation in the cells, deficiency of nutrients such as NHinf4sup+, Mg2+ and POinf4Sup3−was crucial even though cell growth was significantly suppressed.
Abstract: Pseudomonas 135, a facultative methylotroph, was cultivated on methanol as a sole carbon and energy source for the accumulation of poly-β-hydroxybutyric acid (PHB). The cells grew fairly well on minimal synthetic medium containing 0.5% (v/v) of methanol at pH 7.0 and 30° C. The maximum specific growth rate was determined to be 0.26–0.28 h−1 with a growth yield of 0.38 in the optimized growth medium. For stimulation of PHB accumulation in the cells, deficiency of nutrients such as NHinf4sup+, Mg2+ and POinf4sup3−was crucial even though cell growth was significantly suppressed. The PHB content of a 40-h culture was determined to be 37% of the total cell mass in NHinf4sup+-limited medium, 42.5% on Mg2+-deficient medium, and 34.5% on POinf4sup3−-deficient medium. The maximum content of PHB in the cells could reach 55% in NHinf4sup+-limited fed-batch culture. The average relative molecular eight determined by gel permeation chromatography was 3.7 × 105 in NHinf4sup+-limited culture, 2.5 × 105 in Mg2+-deficientmedium, and 3.1 × 105 in POinf4sup3−-deficient medium. Polydispersity determined in each culture was relatively high (about 10–11). The solid PHB had a melting temperature of 173° C.
41 citations