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Halomonas

About: Halomonas is a research topic. Over the lifetime, 742 publications have been published within this topic receiving 21344 citations.


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TL;DR: It is suggested that this bacterium isolated from Mediterranean seawater near a petroleum refinery be assigned to a new genus, at least temporarily, because of the impossibility of finding a single most closely related species.
Abstract: On the basis of phenotypical characteristics and analysis of 16S rRNA sequence, a new species belonging to a new genus is described, and the name Marinobacter hydrocarbonoclasticus is proposed. This organism, isolated from Mediterranean seawater near a petroleum refinery, is a gram-negative, aerobic, rod-shaped bacterium. It grows at NaCl concentrations of 0.08 to 3.5 M and uses various hydrocarbons as the sole source of carbon and energy. Its DNA has a guanine-plus-cytosine content of 52.7 mol%. The 16S rRNA analysis shows a clear affiliation between M. hydrocarbonoclasticus and the gamma group of the phylum Proteobacteria. A close phylogenetic relationship appears among the species Marinomonas vaga, Oceanospirillum linum, Halomonas elongata, and Pseudomonas aeruginosa. Because of the impossibility of finding a single most closely related species, we suggest that this bacterium be assigned to a new genus, at least temporarily. The possibility of a revision of this status when new data appear is, however, not excluded. The type strain is M. hydrocarbonoclasticus SP.17 (= ATCC 49840).

573 citations

Journal ArticleDOI
TL;DR: A combination of morphology, salt tolerance, and guanine plus cytosine content supports the establishment of a new genus, Halomonas, in Family II (Vibrionaceae) of part 8, Gram-Negative Facultatively Anaerobic Bacteria, of Bergey's Manual (8th edition).
Abstract: The morphological, biochemical, and physiological characteristics of nine bacterial strains isolated from a solar salt facility located on Bonaire, Netherlands Antilles are described. The bacteria were gram-negative rods which produce white, opaque colonies on solid media. During the log phase of growth, the cultures consisted of single and paired cells with polar flagella predominating. Older cultures characteristically produced highly elongated, flexible rods. All of these strains reduced NO3 to NO2, grew anaerobically in the presence of NO3, and fermented glucose but oxidized sucrose, glycerol, mannose, and cellobiose. All strains were ornithine and lysine decarboxylase positive, catalase positive, and cytochrome oxidase negative. Eight of the nine strains grew in a complex Casamino Acids liquid medium containing from 0 to 32% (wt/vol) solar salt at temperatures from 23 to 37°C; the ninth strain was restricted in its growth to 0 to 20% solar salt. The guanine plus cytosine content of the deoxyribonucleic acid was 61 ± 1 mol%. This combination of morphology, salt tolerance, and guanine plus cytosine content supports the establishment of a new genus, Halomonas, in Family II (Vibrionaceae) of part 8, Gram-Negative Facultatively Anaerobic Bacteria, of Bergey's Manual (8th edition). The type species of this genus is H. elongata, the type strain of which is isolate 1H9 (= ATCC 33173). Strain 1H15 is regarded as belonging to a biovar of H. elongata on the basis of its production of lophotrichous cells and its inability to grow at 37°c in the presence of 32% solar salt.

405 citations

Journal ArticleDOI
TL;DR: The results of this work should allow researchers to minimise the tests required to arrive at a reliable phenotypic characterisation of Halomonas isolates and to select those of most use to differentiateHalomonas species from each other.

353 citations

Journal ArticleDOI
TL;DR: Alkaliphilic Archaea were relatively closely related to members of the genera Natronococcus and Natronobacterium, and an anaerobic, thermophilic isolate has been assigned to a new genus within the Thermotogales.

289 citations

Journal ArticleDOI
TL;DR: Although most culture collection strains are not able to produce hydrolases, it has been shown that environmental isolates can produce these potentially biotechnological important enzymes.
Abstract: C. S ANCHEZ-PORRO, S. M ARTIN , E. M E L L A D O A N D A. V E N T O S A. 2003. Aims: The aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. Methods and Results: Screening bacteria from different hypersaline environments in South Spain led to the isolation of a total of 122 moderately halophilic bacteria able to produce different hydrolases (amylases, DNases, lipases, proteases and pullulanases). These bacteria are able to grow optimally in media with 5-15% salts and in most cases up to 20-25% salts. In contrast to strains belonging to previously described species, that showed very little hydrolase activities, environmental isolates produced a great variety of hydrolases. These strains were identified as members of the genera: Salinivibrio (55 strains), Halomonas (25 strains), Chromohalobacter (two strains), Bacillus-Salibacillus (29 strains), Salinicoccus (two strains) and Marinococcus (one strain), as well as eight non- identified isolates. Conclusions: Moderately halophilic bacteria are a source of hydrolytic enzymes such as amylases, DNases, lipases, proteases and pullulanases. Significance and Impact of the Study: Although most culture collection strains are not able to produce hydrolases, it has been shown that environmental isolates can produce these potentially biotechnological important enzymes.

267 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202342
2022115
202146
202060
201942
201856