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Hydrolysis

About: Hydrolysis is a(n) research topic. Over the lifetime, 33552 publication(s) have been published within this topic receiving 755971 citation(s).

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Book
01 Feb 1986-

5,277 citations


Book
01 Jan 1976-

4,789 citations


Journal ArticleDOI
Giovanni Bucolo1, Harold David1Institutions (1)
01 May 1973-Clinical Chemistry
TL;DR: A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.

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Abstract: We describe a novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure. Under the conditions of the assay, the enzymatic hydrolysis can be completed in less than 10 min by the combined action of a microbial lipase and a protease. We have been able to demonstrate complete hydrolysis of triglycerides by thin-layer chromatography of the reaction products, by recovery of glycerol from sera of known triglycerides content, and by comparison of triglyceride assays on a number of sera assayed by our method vs. the AutoAnalyzer procedure. The hydrolysis is directly coupled to the enzymatic determination of glycerol, and is followed through absorbance changes at 340 nm. The assay is simple, rapid, and requires only 50 µl or less of sample. Because the enzymes used do not release glycerol from other compounds in serum, the hydrolysis can be considered specific for triglycerides.

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3,044 citations


Book ChapterDOI
TL;DR: This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.

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Abstract: Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.

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1,784 citations


Journal ArticleDOI
12 Jan 2005-Biomacromolecules
TL;DR: Increased acid-to-pulp ratio reduced the dimensions of the nanocrystals thus produced and the critical concentration was increased and the biphasic range became narrower; a suspension made from a bleached kraft eucalyptus pulp gave very similar properties to the softwood nanocrystal suspension when prepared under similar hydrolysis conditions.

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Abstract: Sulfuric acid hydrolysis of native cellulose fibers produces stable suspensions of cellulose nanocrystals. Above a critical concentration, the suspensions spontaneously form an anisotropic chiral nematic liquid crystal phase. We have examined the effect of reaction time and acid-to-pulp ratio on nanocrystal and suspension properties for hydrolyzed black spruce acid sulfite pulp. Longer hydrolysis times produced shorter, less polydisperse black spruce cellulose nanocrystals and slightly increased the critical concentration for anisotropic phase formation. Increased acid-to-pulp ratio reduced the dimensions of the nanocrystals thus produced; the critical concentration was increased and the biphasic range became narrower. A suspension made from a bleached kraft eucalyptus pulp gave very similar properties to the softwood nanocrystal suspension when prepared under similar hydrolysis conditions.

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1,260 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202232
2021769
2020852
2019915
20181,000
2017949

Top Attributes

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Topic's top 5 most impactful authors

John N. Saddler

62 papers, 6.7K citations

Thomas H. Fife

39 papers, 551 citations

Charles E. Wyman

29 papers, 3.5K citations

Guido Zacchi

23 papers, 1.8K citations

Jose M. Guisan

20 papers, 625 citations