Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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TL;DR: The promoter region of the rat ovarian 20 α-HSD gene is identified and partially characterized and it is demonstrated that the regulatory elements for 20α- HSD are present within a 2.5 kb 5′ flanking region of this gene.
16 citations
TL;DR: The enzymatic activities of 17β-HSDs have a gender difference in estrogen formation/metabolism and are distributed around the basement layer of the epidermis irrespective of sex, suggesting the importance for defense from noxae and maintenance of the internal environment.
Abstract: The interconversion of estrone (E1) and 17β-estradiol (E2) is catalyzed by 17β-hydroxysteroid dehydrogenase (17β-HSD) in peripheral steroidogenic organs such as the skin. To inve
16 citations
TL;DR: The data indicate that H SD2 protein and activity levels increase in parallel with the differentiation of alveolar type II epithelial cells in vitro, and that HSD2 protein levels are regulated by 17-β estradiol in male fetal lung tissue.
Abstract: 11-Beta hydroxysteroid dehydrogenase type 2 (HSD2) oxidizes the biologically active glucocorticoid (GC), cortisol, to inactive cortisone We characterized HSD2 gene expression and activity in human adult and fetal lung tissues and in cultured fetal lung explants, and examined the potential regulation of HSD2 in the fetal lung by sex steroids Human adult lung, fetal lung, and cultured fetal lung explant tissues contained similar amounts of HSD2 mRNA However, higher levels of HSD2 protein were detected in human fetal lung tissue than in adult lung, with expression being restricted to a subset of epithelial cells in the fetal lung tissue Differentiated fetal lung explants maintained in culture expressed higher levels of HSD2 protein and enzymatic activity than undifferentiated fetal lung tissues Finally, HSD2 protein levels were decreased in male, but not female, fetal lung explants treated with 17-beta estradiol In contrast, 5-alpha dihydrotestosterone did not significantly affect HSD2 levels These data indicate that HSD2 protein and activity levels increase in parallel with the differentiation of alveolar type II epithelial cells in vitro, and that HSD2 protein levels are regulated by 17-beta estradiol in male fetal lung tissue
16 citations
TL;DR: The porcine 17 beta-hydroxysteroid dehydrogenase type 4 is the key enzyme for the inactivation of estradiol and its localization in peroxisomes was proven by immunogold electron microscopy.
16 citations
TL;DR: The data show that there is a specific change in the gene expression of 3β-HSD in PCO granulosa cells resulting in a suppressed capacity to secrete progesterone.
Abstract: In a preview study we found that luteinizing granulosa cells from follicles of patients with polycystic ovary syndrome (PCOS) have a reduced capacity to synthesize progesterone in vitro. Because the 3β -hydroxysteroid dehydrogenase/δ5-δ4-isomerase (3β -HSD) is an important enzyme for the biosynthesis of progesterone, the reduced capacity of PCO luteinizing granulosa cells to synthesize progesterone in vitro may be due to reduced 3β-HSD gene expression. A reverse transcriptase polymerase chain reaction for 3β -HSD was performed and the relative intensity of signals for 3β-HSD was evaluated using computer-assisted densitometry.Cells from polycystic ovaries expressed less 3β -HSD in follicles ≤10 mm (p < 0.05) and in follicles ≥ 16 mm (p < 0.05) than cells from normal ovaries. Furthermore, after human chorionic gonadotropin stimulus (50 ng/ml), cells from polycystic ovaries expressed less 3 β-HSD in follicles ≥ 16 mm (p < 0.01) than cells from normal ovaries. The data show that there is a specific change in ...
16 citations