Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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TL;DR: A modified method for the radiometric determination of hydroxysteroid dehydrogenase activities in rat liver, with improved precision and a radical reduction in the time involved in performing the assay is described.
Abstract: This paper described a modified method for the radiometric determination of hydroxysteroid dehydrogenase activities in rat liver. The principle advantages of this method are the improved precision and a radical reduction in the time involved in performing the assay. The procedure comprises the following steps: incubation of 14C-labelled substrate with coenzyme and cell fraction under optimized conditions; termination of the reaction by addition of organic solvent containing a defined amount of 3H-labelled reaction product; removal of precipitated protein and coenzyme by centrifugation; paper chromatographic isolation of the product; direct quantitation of 14C activity in the product zone of the paper chromatogram. The assay systems have been applied to elucidate and quantitate sex and strain differences in the activities of the above enzymes in Chbb/THOM and Sprague-Dawley rats.
13 citations
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TL;DR: It is concluded that adrenocortical hormone plays an indirect role in testicular activity in toads during the breeding season.
13 citations
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TL;DR: The albumin present in breast tumor cytosol may be a contributing factor to the observed stimulation of reductive E2DH activity in cultured MCF-7 breast cancer cells.
13 citations
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TL;DR: Investigation of tissue rich in presumed steroid producing cells taken from the brook lamprey during metamorphosis found evidence for hydroxysteroid dehydrogenase activity was obtained from presumed adrenocortical tissue, ovarian and testicular homogenates.
Abstract: Pronephric, opisthonephric, ovarian, and testicular tissue rich in presumed steroid producing cells taken from the brook lamprey during metamorphosis was investigated by histochemical and spectrophotometric methods. Histochemical results seemed to provide evidence for hydroxysteroid dehydrogenase activity using pregnenolone as substrate. Spectrophotometric evidence for hydroxysteroid dehydrogenase activity was obtained from presumed adrenocortical tissue, ovarian and testicular homogenates using pregnenolone, dehydroepiandrosterone, androsterone, and 3 beta, 17 beta-dihydroxy-5 alpha-androstane as substrates.
13 citations
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TL;DR: Results strongly support an additional 17beta-HSD1 binding site for phytoestrogens which is neither the substrate nor the co-factor binding site, and may open new opportunities for the design of inhibitors, not only for 17 beta- HSD1, but also for other family members of the short chain dehydrogenases.
13 citations