Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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TL;DR: It is demonstrated that the mouse 3β–HSD I and VI isoforms can inactivate testosterone within an intact cell milieu, important not only in establishment of structure‐function relationships, but also whenever murine systems are used for developmental/reproductive paradigms associated with human disorders.
Abstract: 3β–Hydroxysteroid dehydrogenase (3β‐HSD) activity is essential for the synthesis of all classes of steroid hormones, converting various Δ5–3β–hydroxysteroids into hormonally active Δ4–3–ketosteroids in NAD+–dependent reactions. Certain 3β–HSD isoforms have been reported to exhibit additional dehydrogenase character (e.g., 17–hydroxysteroid dehydrogenase/reductase). We have investigated whether mouse type I (adrenal/gonadal) and type VI 3β–HSDs (uterine/embryonic) display significant 17β–HSD–like activity. Nonsteroidogenic HEK 293T cells were transiently transfected with pCMV–based expression vectors containing mouse type I and type VI 3β–HSDs. Transfected cells expressing either mouse type I or type VI 3β–HSD converted testosterone to androstenedione, albeit at rates one‐tenth of those of pregnenolone to progesterone in similarly transfected 293T cells. Our findings demonstrate that the mouse 3β–HSD I and VI isoforms can inactivate testosterone within an intact cell milieu. These findings are important no...
9 citations
TL;DR: The investigated 17beta-HSD is the first known fungal pluripotent enzyme of this type and appears to be even better substrates as steroids due to their higher affinity for the enzyme-coenzyme complex and faster conversion of the enzymes- coenzyme-substrate complex into the corresponding products.
9 citations
TL;DR: The results suggest a mechanism by which reduced activity of 3beta-HSD and progesterone by GC by GC during HS might be explained, particularly with the differences in strains observed, and suggests that gonadotropic actions of LH and LH+FSH are countered by the antigonadotropic action of PRL.
9 citations
9 citations
TL;DR: A histochemical method was used for the detection and localization of Δ5-3β- and 17β-hydroxysteroid dehydrogenase activity in the epitheliochorial placenta of the domestic pig, Sus scrofa, and both dehydroepiandrosterone and 3β,17β-dihydroxyandrost-5-ene (androstenediol) were better substrates than pregnenolone, particularly in the earlier stages.
Abstract: A histochemical method was used for the detection and localization of Δ5-3β- and 17β-hydroxysteroid dehydrogenase activity in the epitheliochorial placenta of the domestic pig, Sus scrofa. Placentas were obtained from the fourth week of gestation until term (114 days) and showed much the same substrate specificity throughout pregnancy. Both dehydroepiandrosterone and 3β,17β-dihydroxyandrost-5-ene (androstenediol) were better substrates than pregnenolone, particularly in the earlier stages. 17α- Hydroxypregnenolone was not utilized. With testosterone and estradiol-17β as substrates, the presence of both neutral and phenolic 17β-hydroxysteroid dehydrogenase enzymes could be demonstrated. No activity was seen with estradiol- 17α. The significance of these findings is discussed in relation to the possible role of the placenta in estrogen production in the sow. (Endocrinology 84: 426, 1969)
9 citations