Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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3 citations
TL;DR: The kinetic mechanism of NADP+-dependent 3 alpha-17 beta-hydroxysteroid dehydrogenase and NAD(+)-dependent 3 α(17 beta)-hydroxylsteroid dehydrogenase was studied in both directions as mentioned in this paper.
Abstract: The kinetic mechanism of NADP(+)-dependent 3 alpha-hydroxysteroid dehydrogenase and NAD(+)-dependent 3 alpha(17 beta)-hydroxysteroid dehydrogenase, purified from hamster liver cytosol, was studied in both directions. For 3 alpha-hydroxysteroid dehydrogenase, the initial velocity and product inhibition studies indicated that the enzyme reaction sequence is ordered with NADP+ binding to the free enzyme and NADPH being the last product to be released. Inhibition patterns by Cibacron blue and hexestrol, and binding studies of coenzyme and substrate are also consistent with an ordered bi bi mechanism. For 3 alpha(17 beta)-hydroxysteroid dehydrogenase, the steady-state kinetic measurements and substrate binding studies suggest a random binding pattern of the substrates and an ordered release of product; NADH is released last. However, the two enzymes transferred the pro-R-hydrogen atom of NAD(P)H to the carbonyl substrate.
3 citations
TL;DR: Hydroxysteroid dehydrogenase enzyme activity has been studied histochemically in placental villi maintained in organ culture, some under fully oxygenated conditions and some under conditions of hypoxia, and the process is apparently selective.
3 citations
TL;DR: Portions of frog skin from different regions were examined histochemically for hydroxysteroid dehydrogenase activity and two basic gland types were seen — those possessing activity, thought to be the poison glands, and the mucous glands which were unable to utilize the Hydroxysteroids.
Abstract: Portions of frog skin from different regions were examined histochemically for hydroxysteroid dehydrogenase activity. Two basic gland types were seen — those possessing activity, thought to be the poison glands, and the mucous glands which were unable to utilize the hydroxysteroids.
3 citations
TL;DR: The result demonstrated the presence of the 20α-hydroxysteroid dehydrogenase and Δ4-5α-reductase more or less in all these lines of mammalian cells.
3 citations