Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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TL;DR: Brain weight of 21-day-old rat fetuses positively correlates with the area of the glomerulosa zone, the ratio of this area to the Area of the fasciculata-reticularis zone, and activity of hydroxysteroid dehydrogenase in the glomersulosa and reticularis zones; in the one- day-old newborns these relationships were absent.
Abstract: Brain weight of 21-day-old rat fetuses positively correlates with the area of the glomerulosa zone, the ratio of this area to the area of the fasciculata-reticularis zone, activity of hydroxysteroid dehydrogenase in the glomerulosa zone, and the ratio of its activity in the glomerulosa and reticularis zones. In the one-day-old newborns these relationships were absent.
2 citations
TL;DR: Medroxyprogesterone Acetate Inhibits Pituitary-Independent Steroidogenesis by Inhibiting 3 β-Hydroxysteroid Dehydrogenase Type II (3 β-HSDII)
Abstract: Medroxyprogesterone Acetate (MPA) Inhibits Pituitary-Independent Steroidogenesis by Inhibiting 3 β-Hydroxysteroid Dehydrogenase Type II (3 β-HSDII)
2 citations
TL;DR: Current available crude extracts of Pseudomonas testosteroni (ATCC 11996) are suitable for bile-salt quantification.
Abstract: Recent experimental evidence suggests that, presumably as a result of mutation, crude extracts of Pseudomonas testosteroni (ATCC 11996) now contain amounts of 7α-and 12α-hydroxysteroid dehydrogenate activity that invalidate the results of bile-salt determinations. To confirm or deny this, we studied the specificity of hydroxysteroid dehydrogenase contained in crude extracts of currently available samples of this bacterium in the oxidation of bile-salt hydroxyl groups. The dehydrogenases in these extracts specifically oxidized 3α-hydroxyl groups of cholate, chenodeoxycholate, and deoxycholate anions. No significant amount of 12α-or 7α-hydroxysteroid dehydrogenase activity was detected. Currently available crude extracts of Pseudomonas testosteroni (ATCC 11996) are therefore suitable for bile-salt quantification
2 citations
Patent•
10 Dec 2010
TL;DR: In this article, a method for enzymatic oxidation of 12 alpha -hydroxy steroid, comprising converting the hydroxysteroid in the presence of 12alpha-hydroxysteroid dehydrogenase and cofactor regenerating system as above per se and optionally isolating at least one of the formed oxidation product from the reaction mixture, was presented.
Abstract: Method for cofactor regeneration, comprises redox reaction in which a substrate is converted to corresponding oxidized or reduced product under the use of at least one redox-enabled cofactor and regenerating the used cofactor under the use of an enzymatic cofactor regenerating system comprising (a) a NAD(P)H dehydrogenase with cholic acid tolerance and (b) a redox-mediator, which transfers electrons to molecular oxygen. Independent claims are included for: (1) a method for enzymatic oxidation of 12alpha -hydroxy steroid, comprising converting the hydroxysteroid in the presence of 12alpha -hydroxysteroid dehydrogenase and cofactor regenerating system as above per se and optionally isolating at least one of the formed oxidation product from the reaction mixture; and (2) preparing ursodeoxycholic acid of formula (I), comprising (a1) enzymatically oxidizing cholic acid compounds of formula (II) in the presence of 12alpha -hydroxysteroid dehydrogenase and the cofactor regenerating system as above per se to corresponding 12-keto-chenodeoxycholic acid compounds of formula (III), (b1) deoxygenating (III) to chenodeoxycholic acid of formula (IV), (c1) chemically oxidizing (IV) in position 7 to give 7-keto-lithocholic acid of formula (V), (d1) reducing (V) and (e1) optionally further purifying the reaction product. R : alkyl, NR11R2, H, alkali metal ion or N(R3) 4+>; R3 : H or alkyl; R1 : H or acyl; and R11, R2 : not defined. [Image] [Image] [Image].
2 citations
TL;DR: Derivatives were evaluated biologically in vitro as type 1 enzyme inhibitors in a bacterial cell homogenate as source of recombinant protein and lipophilic substitutions at the 1‐ or 4‐positions in combination with N‐4′‐chlorophenyl substitution were most favourable for activity.
Abstract: 17β-Hydroxysteroid dehydrogenases catalyse interconversion at the C17 position between oxidized and reduced forms of steroidal nuclear receptor ligands. The type 1 enzyme, expressed in malignant cells, catalyses reduction of the less-active estrone to estradiol, and inhibitors have therapeutic potential in estrogen-dependent diseases such as breast and ovarian cancers and in endometriosis. Synthetic decoration of the nonsteroidal N-phenyl-1,2,3,4-tetrahydroisoquinoline (THIQ) template was pursued by using Pomeranz-Fritsch-Bobbitt, Pictet-Spengler and Bischler-Napieralski approaches to explore the viability of this scaffold as a steroid mimic. Derivatives were evaluated biologically in vitro as type 1 enzyme inhibitors in a bacterial cell homogenate as source of recombinant protein. Structure-activity relationships are discussed. THIQs possessing a 6-hydroxy group, lipophilic substitutions at the 1- or 4-positions in combination with N-4'-chlorophenyl substitution were most favourable for activity. Of these, one compound had an IC50 of ca. 350 nM as a racemate, testifying to the applicability of this novel approach.
2 citations