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Hydroxysteroid dehydrogenase

About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.


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Patent
29 Sep 1988
TL;DR: In this paper, a microorganism belonging to the genus Cellulomonas and having the ability to produce 3α-hydroxysteroid dehydrogenase having the following characteristics is cultivated.
Abstract: PURPOSE:To produce 3alpha-hydroxysteroid dehydrogenase having high stability, by cultivating a microorganism, belonging to the genus Cellulomonas and having the ability to produce the 3alpha-hydroxysteroid dehydrogenase having specific properties. CONSTITUTION:A microorganism, belonging to the genus Cellulomonas and having the ability to produce 3alpha-hydroxysteroid dehydrogenase having the following characteristics is cultivated. With reductive ability for steroid compounds having oxo group at the 3-position and carbonyl group-containing compounds, 98,000 molecular weight measured by a native polyacrylamide electrophoresis, 24,000 molecular weight measured by sodium dodecyl sulfate (SDS) electrophoresis and stable when allowed to stand at pH6-8 (1/15M phosphoric acid buffer solution) and 30 deg.C for 24hr and pH7 and 60 deg.C for 2hr. The aimed 3alpha- hydroxysteroid dehydrogenase is then collected from the culture thereof.

1 citations

Patent
04 Mar 2015
TL;DR: In this article, a DNA sequence of a polypeptide chain with 3 alpha-HSD (3 alpha-hydroxysteroid dehydrogenase) activity was revealed.
Abstract: The invention discloses a DNA sequence of a polypeptide chain with 3 alpha-HSD (3 alpha-hydroxysteroid dehydrogenase) activity The DNA sequence comprises a complementary chain sequence The DNA sequence is characterized in that an expression product of the DNA sequence has 3 alpha-hydroxysteroid dehydrogenase activity and is a novel Pseudomonas aeruginosa 3 alpha-hydroxysteroid dehydrogenase The DNA sequence comprises the following contents: a pseudomonas aeruginosa 3 alpha-hydroxysteriod dehydrogenase gene sequence obtained by utilizing bioinformatics analysis, a recombinant expression vector which contains the pseudomonas aeruginosa 3 alpha-hydroxysteroid dehydrogenase gene sequence, a genetically engineered bacterium which expresses the 3 alpha-hydroxysteroid dehydrogenase 3-hsdA and an enzyme kinetics parameter of the 3 alpha-hydroxysteroid dehydrogenase

1 citations

01 Jan 2012
TL;DR: Oestrogens play key roles in the development of the majority of breast tumours, a fact that has been exploited successfully in treating breast cancer with tamoxifen, which is a selective oestrogen.
Abstract: Oestrogens play key roles in the development of the majority of breast tumours, a fact that has been exploited successfully in treating breast cancer with tamoxifen, which is a selective oestrogen ...

1 citations

Journal Article
TL;DR: Direct studies with histochemical localization of steroidogenic enzymes and hepatocyte glycogen content revealed that in state of adrenal suppression, PGE2 fails to elicit a positive change in steroid output from adrenal cortex, although apparently increases the enzymatic staining reactions.
Abstract: Long term Prostaglandin E2 treatment stimulates adrenal weight and steroid output. Indirect studies with histochemical localization of steroidogenic enzymes and hepatocyte glycogen content revealed that in state of adrenal suppression, PGE2 fails to elicit a positive change in steroid output from adrenal cortex, although apparently increases the enzymatic staining reactions. This suggests that the action of Prostaglandin on adrenal steroidogenesis is a pharmacologic one rather than a physiologic response.

1 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202319
202217
20218
202016
201916
20186