Topic
Hydroxysteroid dehydrogenase
About: Hydroxysteroid dehydrogenase is a research topic. Over the lifetime, 1087 publications have been published within this topic receiving 28468 citations. The topic is also known as: hydroxysteroid dehydrogenase.
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TL;DR: The results of this study suggest that the 3- β -hydroxysteroid dehydrogenase and the isomerase might be separate enzymes and that there might be distinct isomerases for different substrates in the particulate fractions.
48 citations
TL;DR: The multispecificity demonstrated by 17beta-HSDs is important for steroid metabolism in gonadal and peripheral tissues, and is a consequence of the architecture of their binding and catalytic sites.
48 citations
TL;DR: Ovarian tissues from the ovoviviparous elasmobranch, Squalus acanthias were examined histochemically for the distribution of NAD and NADP diaphorase, glucose- 6-phosphate dehydrogenase, G-6-PDH, and 3βHSD and positive reactions were obtained in all tissues tested.
47 citations
TL;DR: The ability of soy isoflavones to regulate androgen biosynthesis in Leydig cells is due in part to action on Leydigs cell 3beta-HSD activity, implying that the increasing intake of soy-based food products and their potential effect on blood androgen levels are greatly relevant to public health.
Abstract: The objective of the present study was to investigate the effects of genistein and equol on 3β-hydroxysteroid de- hydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase 3 (17β-HSD3) in human and rat testis microsomes. These enzymes (3β-HSD and 17β-HSD3), along with two others (cytochrome P450 side-chain cleavage enzyme and cytochrome P450 17α-hydroxylase/17-20 lyase), catalyze the reactions that convert the steroid cholesterol into the sex hormone testosterone. Genistein inhibited 3β-HSD activity (0.2 µmol L -1 pregnenolone) with half-maximal in- hibition or a half-maximal inhibitory concentration (IC50) of 87 ± 15 (human) and 636 ± 155 nmol L -1 (rat). Genistein's mode of action on 3β-HSD activity was competitive for the substrate pregnenolonrge and noncompetitive for the co- factor NAD + . There was no difference in genistein's potency of 3β-HSD inhibition between intact rat Leydig cells and testis microsomes. In contrast to its potent inhibition of 3β-HSD, genistein had lesser effects on human and rat 17β-HSD3 (0.1 µmol L -1 androstenedione), with an IC50 ≥ 100 µmol L -1 . On the other hand, equol only inhibited hu- man 3β-HSD by 42%, and had no effect on 3β-HSD and 17β-HSD3 in rat tissues. These observations imply that the ability of soy isoflavones to regulate androgen biosynthesis in Leydig cells is due in part to action on Leydig cell 3 β- HSD activity. Given the increasing intake of soy-based food products and their potential effect on blood androgen levels, these findings are greatly relevant to public health.
47 citations
TL;DR: The synthesis and SAR studies of 3- and 4-substituted 7-hydroxycoumarins as novel 17beta-HSD3 inhibitors are discussed and the most potent compounds from this series exhibited low nanomolar inhibitory activity with acceptable selectivity versus other 17 beta- HSD isoenzymes and nuclear receptors.
47 citations