Topic
Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: Immobilized trypsin (TR) was more stable than the free one and demonstrated higher enzymatic activity at elevated temperatures (45-55°C) and in the alkaline pH region (6-10.5) while Fe3O4 NPs-GA-TR retained about 64% of its initial activity during the same storage period.
114 citations
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TL;DR: In this article, the ability of immobilized lipase from Candida antarctica (Novozym 435) to catalyze the alcoholysis of canola oil and methanol was investigated.
Abstract: The ability of immobilized lipase from Candida antarctica (Novozym 435) to catalyze the alcoholysis of canola oil and methanol was investigated. Response surface methodology (RSM) and five–level–five–factor central composite rotatable design (CCRD) were employed to evaluate the effects of synthesis parameters, such as reaction time, temperature, enzyme concentration, substrate molar ratio of methanol to canola oil, and added water content on percentage weight conversion of canola oil methyl ester by alcoholysis. Reaction temperature and enzyme concentration were the most important variables. High temperature and superabundant methanol inhibited the ability of Novozym 435 to catalyze the synthesis of biodiesel. Based on the analysis of ridge max, the optimum synthesis conditions were as follows: reaction time 12.4 h, temperature 38.0 °C, enzyme concentration 42.3%, substrate molar ratio 3.5:1, and added water 7.2%. The predicted value was 99.4% weight conversion, and the actual experimental value was 97.9% weight conversion. Copyright © 2004 Society of Chemical Industry
114 citations
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TL;DR: In this article, the structural characterization of the glucose oxidase (GOD) immobilized membrane was performed in detail, i.e., the fraction of Silk I and Silk II of the membrane.
114 citations
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TL;DR: In this paper, a large-pore nanoporous material, SBA-15, was functionalized with organosilanes by co-condensation method in the presence of nonionic triblock copolymer P123 as a template under acidic conditions.
114 citations
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TL;DR: Nickel nanoparticles can be successfully used in the construction of electrochemical enzyme sensors for the detection of phenolic compounds and are demonstrated to provide comparable or better characteristics in terms of detection limit and sensitivity than Fe(3)O(4) and gold nanoparticles.
114 citations