Topic
Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: In this paper, a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator was used to grow stimuli responsive polymers from the surface of a protein.
104 citations
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TL;DR: In this article, α-amylase was applied to alumina via adsorption, and the results showed that the enzyme was getting adsorbed only on the external surface of the support, which may be due to interparticle diffusional mass transfer restrictions.
104 citations
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TL;DR: In this paper, microfabricated sensors for determination of creatine and creatinine in serum were described, based on a bienzyme sequence, involving creatine amidinohydrolase (CI) and sarcosine oxidase (SO).
104 citations
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TL;DR: Magnetic Fe3O4-cellulose-chitosan hybrid gel microspheres were prepared by sol-gel transition technology using ionic liquids as solvent for cellulose and chitosans dissolution and regeneration.
Abstract: Magnetic Fe3O4–cellulose–chitosan hybrid gel microspheres were prepared by sol–gel transition technology using ionic liquids as solvent for cellulose and chitosan dissolution and regeneration. The synthesized microspheres were characterized by XRD, FTIR, SEM, and vibrating sample magnetometer (VSM). The XRD and FTIR results showed that chitosan and cellulose had been successfully coated onto the surface of Fe3O4 after the preparation. SEM presented that the synthesized microspheres were regular sphere with a mean diameter of about 10 μm. Furthermore, it was found that glucose oxidase (GOx) could be successfully immobilized on the hybrid gel microspheres via glutaraldehyde technique. The immobilized enzyme presented higher thermostability, wider range of pH optima and improved storage stability in comparison with free GOx. The glucose conversion could reach 91.5% within 4 h and retained 84.2% of the initial activity after 15 cycles of use. These results demonstrate their potential applications in the field of biocatalysis.
104 citations
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TL;DR: The results demonstrate that the diffusional limitation induced by the aggregation of carbon nanoparticles cannot be ignored because it can lead to increased reaction times, low efficiency, and high economic costs when low concentrations of environmental contaminants are used.
104 citations