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Immobilized enzyme

About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.


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Journal ArticleDOI
TL;DR: In this paper, the authors described the optimization of the esterification reaction of butyl acetate synthesis catalyzed by Candida antarctica lipase B (Novozym 435).

104 citations

Journal ArticleDOI
TL;DR: To investigate the concentration effects of immobilized biosignal molecules by microscopic observation, a gradient micropattern immobilization technique using a photomask was devised and growth enhancement was observed only in dense EGF derivative immobilized regions.

103 citations

Journal ArticleDOI
01 Jan 1988-Analyst
TL;DR: A biosensor for the continuous determination of glucose was presented and the consumption of oxygen was measured by following, via fibre optic bundles, the changes in the fluorescence of an oxygen-sensitive dye whose fluorescence is quenched dynamically by molecular oxygen.
Abstract: A biosensor for the continuous determination of glucose is presented Glucose oxidase was immobilised covalently on a nylon membrane and the consumption of oxygen was measured by following, via fibre optic bundles, the changes in the fluorescence of an oxygen-sensitive dye whose fluorescence is quenched dynamically by molecular oxygen The dye is dissolved in a very thin silicone membrane placed beneath the enzyme layer As a result of the oxidation by the enzyme a certain amount of oxygen is consumed This amount is indicated by the fluorescent dye The measurements were performed in flowing air-saturated solutions containing 01 M pH 70 phosphate buffer The effects of the amount of immobilised enzyme and the thickness of the indicator layer on response times (t90= 1–6 min), analytical ranges (01–20 mM) and relative signal changes (up to 26%) were investigated

103 citations

Journal ArticleDOI
TL;DR: The efficiencies of the three immobilized enzyme structures as reagents for the automated determination of their substrates were compared and an improved process is described for the immobilization of glucose oxidase and urease on the inside surface of partially hydrolysed nylon tube.
Abstract: 1. Glucose oxidase (EC 1.1.3.4) and urease (EC 3.5.1.5) were covalently attached through glutaraldehyde to low-molecular-weight nylon powder. 2. Immobilized derivatives of glucose oxidase and urease were prepared by cross-linking the respective enzymes within the matrix of a nylon membrane. 3. An improved process is described for the immobilization of glucose oxidase and urease on the inside surface of partially hydrolysed nylon tube. 4. Automated analytical procedures are described for the determination of glucose with each of the three immobilized glucose oxidase derivatives and for the determination of urea with each of the three immobilized urease derivatives. 5. The efficiencies of the three immobilized enzyme structures as reagents for the automated determination of their substrates were compared.

103 citations

Journal ArticleDOI
TL;DR: The immobilized GOD exhibits a significantly improved stability during storage in buffer solution over that of the free enzyme and the optimum pH value of GOD was not affected by the immobilization reaction and the pH stability range was considerably widened.

103 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023219
2022417
2021480
2020548
2019553
2018543