Topic
Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: Free as well as alginate immobilized urease was utilized for detection and quantitation of cadmium (Cd2+) in aqueous samples revealing a clear dependence on the concentration of Cd2+.
96 citations
TL;DR: The immobilization process brought significant enhancement of stability to temperature and extreme pH values with respect to free papain and the temperature profile of this form of immobilized papain showed a broad range of activity compared with both free and immobilized form of papain in the absence of cysteine.
Abstract: Immobilization of papain on Sepharose 6B in the presence of different concentrations of cysteine affected the enzyme activity depending on cysteine concentration. The maximum specific activity was observed when papain was immobilized with 200 mM cysteine. The immobilization process brought significant enhancement of stability to temperature and extreme pH values with respect to free papain. After immobilization, the optimum temperature of papain activity increased by 20 degrees C (from 60 to 80 degrees C) and its optimum pH activity shifted from 6.5 to 8.0. Catalytic efficiency (k(cat)/K(m)) and specific activity of the immobilized enzyme do not significantly change after immobilization. The temperature profile of this form of immobilized papain showed a broad range of activity compared with both free and immobilized form of papain in the absence of cysteine. This significant behavior in terms of activation energy is also discussed.
96 citations
TL;DR: Experimental results indicated that the assembled calcium phosphate shell significantly enhanced the mechanical stability and lowered the swelling degree of the capsule, additionally improved the immobilization efficiency and dramatically inhibited the leakage of β-galactosidase.
96 citations
TL;DR: This review provides an overview of various carriers for enzyme immobilization, with the primary focus on food industry.
Abstract: Enzymes are macromolecular biocatalysts, widely used in food industry. In applications, enzymes are often immobilized on inert and insoluble carriers, which increase their efficiency due to multiple reusability. The properties of immobilized enzymes depend on the immobilization method and the carrier type. The choice of the carrier usually concerns the biocompatibility, chemical and thermal stability, insolubility under reaction conditions, capability of easy regeneration and reusability, as well as cost efficiency. In this review, we provide an overview of various carriers for enzyme immobilization, with the primary focus on food industry.
96 citations
TL;DR: Results show that chitosan activated with divinyl sulfone is a very promising support for enzyme immobilization and the proposed protocol is able to successfully improve enzyme stability.
96 citations