Topic
Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: The results indicated that alcoholysis products of cotton seed oil could be used as valuable intermediates in oleochemistry.
289 citations
TL;DR: This review attempts to provide an updated compilation of the studies reported on biodiesel production by using lipase immobilized through various techniques and the parameters, which affect their functionality.
Abstract: Increase in volume of biodiesel production in the world scenario proves that biodiesel is accepted as an alternative to conventional fuel. Production of biodiesel using alkaline catalyst has been commercially implemented due to its high conversion and low production time. For the product and process development of biodiesel, enzymatic transesterification has been suggested to produce a high purity product with an economic, environment friendly process at mild reaction conditions. The enzyme cost being the main hurdle can be overcome by immobilization. Immobilized enzyme, which has been successfully used in various fields over the soluble counterpart, could be employed in biodiesel production with the aim of reducing the production cost by reusing the enzyme. This review attempts to provide an updated compilation of the studies reported on biodiesel production by using lipase immobilized through various techniques and the parameters, which affect their functionality.
289 citations
TL;DR: The development of effective methods for immobilizing large multi-subunit proteins with multiple covalent linkages (multi-point immobilization) has been effective in stabilizing proteins where subunit dissociation is the initial step in enzyme inactivation.
288 citations
TL;DR: The developed biosensor responds efficiently to glucose presence over the concentration range 5-1270 μM with the detection limit 1.73 μM (S/N=3) and sensitivity 0.085 μA μM(-1) cm(-2), attributed to the large surface-to-volume ratio, excellent biocompatibility of GQD, porosity of G QD|CCE, and the abundance of hydrophilic edges as well as hydrophobic plane in
287 citations
TL;DR: An amperometric enzyme electrode for hydrogen peroxide was developed via easy and effective enzyme immobilization using the sol-gel technique in this article, which comprises horseradish peroxidase (HRP) immobilized in the silica solgel matrix on a carbon paste electrode.
286 citations