Topic
Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: The immobilized L-ASNase showed significantly higher stability when the temperature raised to 40-50 degrees C, it showed preferable resistance to trypsin digestion as compared with native enzyme, and the bioconjugation of L- ASNase widened the optimum reactive temperature range of the enzyme.
178 citations
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TL;DR: The synthesis, characterization, development and applications of organic-inorganic hybrid nanoflowers formed of various enzymes and metal ions are discussed and potential mechanism underlying enhanced catalytic activity and stability is explained.
177 citations
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TL;DR: In this article, the activation of an amine porous silica with glutaraldehyde has been studied and compared with the capacity of the activated silica to bind trypsin, based on a reaction mechanism with a polymeric form resulting from aldol condensation.
177 citations
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TL;DR: A novel nanocomposite consisting of cellulose nanocrystals functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization with phenomenally high loading is presented in this work and is anticipated to extend to other enzymes.
Abstract: A novel nanocomposite consisting of cellulose nanocrystals (CNCs) functionalized with gold nanoparticles (AuNPs) serving as an excellent support for enzyme immobilization with phenomenally high loading is presented in this work. As testing models, cyclodextrin glycosyl transferase (CGTase) and alcohol oxidase were conjugated on an activated CNC/AuNP matrix. This catalytic platform exhibits significant biocatalytic activity with excellent enzyme stability and without apparent loss of the original activity. The recovered specific activities were ∼70% and 95% for CGTase and alcohol oxidase, respectively. This novel and inexpensive material is anticipated to extend to other enzymes, enhancing the enzyme loading and activity as well as the stability in both operation and storage.
177 citations
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TL;DR: The potential applications of magnetic particles in several biomedical and biotechnology fields are discussed and the binding of the proteins and enzymes to magnetic particles was confirmed.
175 citations