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Immobilized enzyme
About: Immobilized enzyme is a research topic. Over the lifetime, 15282 publications have been published within this topic receiving 401860 citations.
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TL;DR: In this paper, the authors used tetraethylorthosilicate (TEOS) and 3-aminopropyltriethoxysilane (APTES), 3-mercaptopropyltrimethoxylynne (MPTMS), phenyltrinethoxymethylthoxyselane (PTMS), vinyltrieethoxisilane(VTES), and 4-triethoxyll)butyronitrile (TSBN) as support materials for enzyme penicillin G acylase
152 citations
TL;DR: It was demonstrated that the immobilized enzyme could be used repeatedly, after intermittent storage, for production of L-DOPA (3,4-dihydroxyphenylalanine) and Cu-alginate entrapped tryosinase was found to be superior in this regard.
151 citations
TL;DR: This lipase immobilization method shows the best performance among various immobilized lipase systems using the same source of lipase and substrate when considering protein loading, activity retention, and kinetic parameters.
Abstract: Polyacrylonitrile (PAN) nanofibers could be fabricated by electrospinning with fiber diameter in the range of 150–300 nm, providing huge surface area for enzyme immobilization and catalytic reactions. Lipase from Candida rugosa was covalently immobilized onto PAN nanofibers by amidination reaction. Aggregates of enzyme molecules were found on nanofiber surface from field emission scanning electron microscopy and covalent bond formation between enzyme molecule and the nanofiber was confirmed from FTIR measurements. After 5 min activation and 60 min reaction with enzyme-containing solution, the protein loading efficiency was quantitative and the activity retention of the immobilized lipase was 81% that of free enzyme. The mechanical strength of the NFM improved after lipase immobilization where tensile stress at break and Young's modulus were almost doubled. The immobilized lipase retained >95% of its initial activity when stored in buffer at 30 °C for 20 days, whereas free lipase lost 80% of its initial activity. The immobilized lipase still retained 70% of its specific activity after 10 repeated batches of reaction. This lipase immobilization method shows the best performance among various immobilized lipase systems using the same source of lipase and substrate when considering protein loading, activity retention, and kinetic parameters.
151 citations
TL;DR: Polyethyleneimine (PEI), with the highest concentration of amino groups, has found acceptance as a carrier in a number of industrial immobilized biosystems and is claimed to highlight the potential of PEI.
151 citations
TL;DR: A novel method for the immobilization of penicillin G acylase involves the physical aggregation of the enzyme, followed by chemical cross-linking to form insoluble cross-linked enzyme aggregates (CLEAs) that possess a high specific activity as well as a high productivity and synthesis/hydrolysis ratio in the synthesis of semi-synthetic antibiotics in aqueous media.
Abstract: A novel method for the immobilization of penicillin G acylase (penicillin amidohydrolase, EC 35111) is reported It involves the physical aggregation of the enzyme, followed by chemical cross-linking to form insoluble cross-linked enzyme aggregates (CLEAs) Compared with conventionally immobilized penicillin G acylases, these CLEAs possess a high specific activity as well as a high productivity and synthesis/hydrolysis (S/H) ratio in the synthesis of semi-synthetic antibiotics in aqueous media Moreover, they are active in a broad range of polar and apolar organic solvents
150 citations