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Incubation
About: Incubation is a research topic. Over the lifetime, 5748 publications have been published within this topic receiving 126541 citations.
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TL;DR: The results show that BA not only has an important effect on the different phases of micropropagation, but will also regulate the future development of the regenerants.
Abstract: Actinidia deliciosa apical shoots were cultured in MS liquid medium with cellulose plugs as support for the explants. Different BA (4.4 μM) incubation periods were tested in order to improve the effectiveness of the micropropagation system by reducing the cytokinin incubation period. At the end of 3 successive subcultures, the explants were analysed and a number of parameters (number, weight and length of shoots, presence and weight of callus, multiplication index, etc.) were measured. Different BA incubation periods have a long-term effect since the best results at the end of multiplication stage were not followed by better growth at the end of the acclimatised period studied. The highest quality plants were those obtained from culturing in the presence of BA for 1 day. Our results show that BA not only has an important effect on the different phases of micropropagation, but will also regulate the future development of the regenerants.
39 citations
TL;DR: Concentrations of LH and FSH were generally higher during the early part of the breeding season than during late incubation and leading, whereas FSH concentrations in female swans did not change during this period.
39 citations
TL;DR: The feces of some normal humans were previously shown to be mutagenic by the Salmonella mutagenicity assay with strain TA100, but this appears to be due to a single type of compound which may be produced by anaerobic bacteria.
Abstract: The feces of some normal humans were previously shown to be mutagenic by the Salmonella mutagenicity assay with strain TA100. In the present study, the mutagenicity of feces of certain donors can be increased by anaerobic incubation for 96 h. The increase in mutagenicity did not occur upon incubation in the cold or in air, in the presence of antimicrobial agents or it the feces were sterilized by heat. On thin-layer chromotographs, the relative mobility of fecal mutagen from all donors after incubation was the same in any one of 4 different solvent systems. The major mutagenicity appears to be due to a single type of compound which may be produced by anaerobic bacteria.
39 citations
TL;DR: In vitro antifungal susceptibility testing for filamentous fungi remains unstandardized and is unreliable for determining adequate therapy, while the combined effects of inoculum size and time of read and of time of reading and temperature produced systematic variation in MICs.
Abstract: In vitro antifungal susceptibility testing for filamentous fungi remains unstandardized and is unreliable for determining adequate therapy. A study was performed to evaluate the effect of inoculum size (10(2), 10(3), 10(4), and 10(5) conidia/ml), incubation time (48 and 72 h), and temperature (25, 30, and 35 degrees C) on MICs of amphotericin B for Fusarium spp. (20 strains). The inoculum size showed the clearest effect: when the inoculum was varied from 10(2) to 10(5) conidia/ml, the geometric mean MICs showed increases of between 10- and 19-fold in all the combined conditions of temperature and incubation time assayed. Time of incubation had less effect (increases of between two- and threefold in approximately half of the geometric mean MICs), and temperature especially had little effect (the increases were no higher than twofold). The effects of interaction between inoculum size and temperature on MICs were not statistically significant, while the combined effects of inoculum size and time of reading and of time of reading and temperature produced systematic variation in MICs.
39 citations
TL;DR: The age of eggs is one factor which must be considered when conductance of eggs of conspecific populations is compared between habitats or over altitudinal gradients.
Abstract: In contrast to previously published observations of constant daily water loss during incubation in some large, precocial eggs, daily rates of water loss increase approximately 42% between days 1–4 in redwinged blackbird eggs and conductance to water vapor increases 47 and 48% during the early stages of incubation in red-winged blackbird and robin eggs, respectively. Therefore, the age of eggs is one factor which must be considered when conductance of eggs of conspecific populations is compared between habitats or over altitudinal gradients.
39 citations