Topic
Incubation
About: Incubation is a research topic. Over the lifetime, 5748 publications have been published within this topic receiving 126541 citations.
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TL;DR: Insight is provided into the biological and physical factors that may be important in generating variability in bacterial population sizes among leaves, and they have important implications for the design of appropriate strategies for sampling leaf surface microbial populations.
Abstract: The influences of plant species and plant incubation conditions on the variability in bacterial population sizes among leaves were investigated in field and growth chamber studies Pseudomonas syringae strains TLP2 and Cit7 were inoculated onto plants and population sizes were measured at intervals after inoculation Total bacterial population sizes were also assessed in field studies Levels of leaf-to-leaf variability in both P syringae population size and bacterial community size differed significantly among plant species For all plant species, variability among leaves in population sizes of inoculated bacteria was consistently greater than the leaf-to-leaf variability in numbers of total bacteria Considering levels of variability in population size immediately prior to and following incubation under either wet or dry physical conditions, leaf-to-leaf variability in the population sizes of inoculated P syringae strains increased significantly following incubation under dry, but not under wet, conditions Measurements of leaf-to-leaf variability immediately prior to and following incubation were positively correlated regardless of whether the incubation was under wet or dry conditions, though the correlation was greater following dry incubation These data provide insight into the biological and physical factors that may be important in generating variability in bacterial population sizes among leaves, and they have important implications for the design of appropriate strategies for sampling leaf surface microbial populations
113 citations
TL;DR: Methods for the fine structural demonstration of acid phosphatase were studied in monolayers ofin vitro cultured cells after fixation with glutaraldehyde, finding that with short fixation times, the composition of the washing and incubation solutions was of major importance.
Abstract: 1.
Methods for the fine structural demonstration of acid phosphatase were studied in monolayers of in vitro cultured cells after fixation with glutaraldehyde.
2.
Inactivation of enzyme activity occurred rapidly during the initial phase of glutaraldehyde fixation.
3.
Fixation for more than 5 min did not cause further marked inactivation of enzyme activity.
4.
Stabilization of the cells for cytochemical incubations required a fixation for at least 30 min in glutaraldehyde.
5.
The total osmolality of the fixative was of minor importance, in contrast to the major importance of effective osmolality, for obtaining optimum cytochemical and ultra-structural results.
6.
Following proper fixation, the osmotic strength of the washing and incubation solutions was not critical.
7.
With short fixation times, the composition of the washing and incubation solutions was of major importance.
8.
Dimethyl sulphoxide in washing and incubation media was effective in shortening incubation times (thereby preventing the occurrence of unspecific precipitates and derangement of fine structure).
113 citations
TL;DR: In this paper, a qualitative and quantitative scoring of day-old chicks is proposed to evaluate the hatchability and post-hatching growth potential of the hatched chicks, which is not reflected in any of the actual scoring systems for chick quality.
112 citations
TL;DR: Large eggs produced large hatchlings that were larger than their siblings at 120 days of age, dependent upon egg size and incubation condition, and wet sand is lethal for desert tortoise eggs.
Abstract: Incubation temperature has a direct effect on sex determination of the desert tortoise. Low temperatures (26.0-30.6 C) produce males and high temperatures (32.8-35.3 C) produce females. Pivotal temperature is approximately 31.8 C. Macroscopic and microscopic anatomy of the gonads is similar to that of other turtles. Hatching success and survival is very good between 28.1 and 32.8 C in dry sand (-5000 kPa). Incubation at 35.3 C is lethal for 72% of the eggs and produces weak hatchlings that die within 45 days. Wet sand (-5 kPa) is lethal for desert tortoise eggs. Hatchling size was dependent upon egg size and incubation condition. Hatchlings from eggs incubated at 32.8 and 35.3 C were significantly smaller than hatchlings from eggs incubated at 28.1 and 30.6 C. Hatching mass had no effect on growth rate of hatchlings. Thus, large eggs produced large hatchlings that were larger than their siblings at 120 days of age. Hatchlings from
111 citations
TL;DR: The results suggest that in acute diabetes mellitus patients, HSA, after 21 days of glycation, passes through a molten globule-like state and may contribute to the pathogenesis of diabetes, and perhaps other diseases.
111 citations