Incubation

About: Incubation is a research topic. Over the lifetime, 5748 publications have been published within this topic receiving 126541 citations.

Influence of temperature stimulation during the last 4 days of incubation on secondary sex ratio and later performance in male and female broiler chicks

Abstract: 1. In 6 incubation trials a total of 9883 eggs (Ross 308) were incubated from d 1 to 17 under normal incubation conditions (37.2-37.4 degrees C) and then sorted into three hatch incubators (control: 37.2-37.4 degrees C; chronic warm incubation: 38.2-38.4 degrees C, 24 h daily; short-term warm stimulation: 38.2-38.4 degrees C, 2 h daily) in incubation trials 1 and 2 or two hatch incubators (control and short-term warm stimulation) in trials 3-6. 2. The one-day-old chicks were selected by sex and chick quality was analysed in random samples using the Pasgar score. A total of 120 male and 120 female one-day-old chickens from each incubator were used for a 35-d fattening period. 3. Neither chronic nor short-term increase in incubation temperature had a negative effect on hatchability and chick quality. Short-term warm stimulation improved hatchability by more than 1.5% and was associated with a significantly higher proportion of hatched male chicks. 4. In the subsequent broiler growth trial, the mean daily weight gain of the short-term warm stimulated male broiler chicks was significant higher than for the control group, which results in a body weight increase of 2.9%. 5. Feed conversion (feed:gain ratio) of the short-term warm stimulated male and female broilers was significantly lower than in the males and females of the control and chronic warm incubated groups. 6. In conclusion, an incubation temperature profile which includes short-term temperature variation can be important in improving poultry performance (European patent pending since March 2008).

Factors Affecting the Toxicity of Methylmercury Injected into Eggs

Abstract: We developed a standardized protocol for comparing the sensitivities of the embryos of different bird species to methylmercury when methylmercury was injected into their eggs. During the course of developing this protocol, we investigated the effects of various factors on the toxicity of the injected methylmercury. Most of our experiments were done with chicken (Gallus domesticus), mallard (Anas platyrhynchos), and ring-necked pheasant (Phasianus colchicus) eggs, all of which were purchased in large numbers from game farms. A smaller amount of work was done with double-crested cormorant (Phalacrocorax auritus) eggs collected from the wild. Several solvents were tested, and corn oil at a rate of 1 μl/g egg contents was selected for the final standardized protocol because it had minimal toxicity to embryos and because methylmercury dissolved in corn oil yielded a dose–response curve in a range of egg concentrations that was similar to the range that causes reproductive impairment when the mother deposits methylmercury into her own eggs. The embryonic stage at which eggs were injected with corn oil altered mercury toxicity; at early stages, the corn oil itself was toxic. Therefore, in the final protocol we standardized the time of injection to occur when each species reached the morphologic equivalent of a 3-day-old chicken embryo. Although solvents can be injected directly into the albumen of an egg, high embryo mortality can occur in the solvent controls because of the formation of air bubbles in the albumen. Our final protocol used corn oil injections into the air cell, which are easier and safer than albumen injections. Most of the methylmercury, when dissolved in corn oil, injected into the air cell passes through the inner shell membrane and into the egg albumen. Most commercial incubators incubate eggs in trays with the air cell end of the egg pointing upward, but we discovered that mercury-induced mortality was too great when eggs were held in this orientation. In addition, some species of bird eggs require incubation on their sides with the eggs being rolled 180° for them to develop normally. Therefore, we adopted a procedure of incubating the eggs of all species on their sides and rolling them 180° every hour. Little has been published about the conditions of temperature, humidity, and the movements to which eggs of wild birds need to be subjected for them to hatch optimally under artificial incubation. Not unexpectedly, hatching success in an artificial incubator is generally less than what natural incubation by the parents can achieve. However, the survival of control embryos of most wild bird species was good (generally ≥ 80%) up to within 1 or 2 days of hatching when we incubated the eggs at 37.5°C (or 37.6°C for gallinaceous species) at a relative humidity that resulted in an approximate 15% to 16% loss in egg weight by the end of incubation and by incubating the eggs on their sides and rolling them 180°/h. To improve statistical comparisons, we used survival through 90% of incubation as our measurement to compare survival of controls with survival of eggs injected with graded concentrations of mercury.

Fluorodeoxyglucose Uptake In Vitro: Aspects of Method and Effects of Treatment with Gemcitabine

Abstract: Rat prostate adenocarcinoma cells were used to evaluate different incubation procedures for the measurement of fluorodeoxyglucose (FDG) uptake and to measure the effects on chemotherapy. Methods: The cells were incubated for 10 or 60 min in media with different glucose concentrations. Furthermore, the cells were treated for 4 hr with different doses of gemcitabine. FDG uptake was measured immediately and 4 hr after treatment. The FDG transport was determined with a zero-trans assay, as well as the messenger RNA (mRNA) content of the glucose transporter type 1 (GLUT1) and the hexokinase assay (HK). Results: A decrease in FDG uptake with increasing cell number after 60 min of incubation in all media was found. The shorter incubation time yielded more stable uptake data. The glucose content in the medium decreased with increasing cell number and incubation time, which showed that the glucose-to-FDG ratio is not constant in assays that use glucose-containing media. Treatment with gemcitabine resulted in an increase in FDG uptake with increasing dose and time after the end of therapy. Incubation experiments with 3H-inulin revealed that the changes were not caused by unspecific membrane alterations. The affinity (Km) of the transport system remained unchanged, whereas the maximum velocity (Vmax) increased. However, the mRNA content for GLUT1 and HK was unchanged. Conclusion: With these data in mind, an uptake procedure was suggested in a glucose-free medium with an end concentration of 0.1 mM FDG or a zero-trans assay to determine Vmax and Km of the transport system. In FDG-PET studies on patients with tumors, these in vitro data may be helpful to monitor and optimize the therapeutic outcome by combining the chemotherapeutic agent with low doses of deoxyglucose.

Patterns of nest attendance in female wood ducks

Abstract: We examined sources of variation in incubation patterns among female Wood Ducks (Aix sponsa), and investigated the effect of female nest attentiveness on incubation period. Data were collected from 44 females (n = 911 days) using temperature data loggers to monitor nest attendance throughout incubation. Mean (± SE) incubation constancy was 86.9 ± 0.6% and incubation period averaged 30.9 ± 0.2 days. Females took an average of two bimodally-distributed recesses per day. Duration of recesses averaged 98.6 ± 3.4 min, but were shorter in the morning than in mid-day or late afternoon. Body mass of incubating females declined 0.68 ± 0.2 g day−1, but there was no relationship between constancy and early incubation body mass or weight change of females. Incubation constancy was not correlated with length of the incubation period. For most females, incubation constancy and recess frequency did not change as incubation progressed. The fact that incubating females only lost an average of 3% of body mass, and...