Topic
Indole alkaloid
About: Indole alkaloid is a research topic. Over the lifetime, 868 publications have been published within this topic receiving 15401 citations. The topic is also known as: indole alkaloids.
Papers published on a yearly basis
Papers
More filters
TL;DR: The total synthesis of the tetracyclic indole alkaloid ht-13-A from commercially available (−)-4-amino-2-hydroxybutyric acid was reported in this article.
33 citations
TL;DR: Three undescribed indole alkaloid derivatives, indigodoles A-C, along with seven known compounds were isolated from this preparation of Qing Dai and their structures were elucidated from spectroscopic data, including NMR, MS, UV, IR, optical rotation, and CD.
33 citations
TL;DR: A hexacylic indole alkaloid, tronoharine, was obtained from the stem-bark extract of Tabernaemontana corymbosa and its structure elucidated by spectral analysis as discussed by the authors.
33 citations
Journal Article•
TL;DR: Cell suspension cultures of Catharanthus roseus, Tabernaemontana divaricata, Taberellonia pandacaqui, Cinchona robusta, Rubia tinctorum and Euonymus europaeus, representing 3 different plant families, were compared with respect to indole alkaloid production and specific activities of the enzymes.
32 citations
TL;DR: It is confirmed that over-expression of Crtdc is a superlative method to improve the biosynthetic potential of Rauwolfia hairy root cultures and Enhanced reserpine and ajmalicine production can serve as an alternative choice to provide resources for relative pharmaceutical industries.
Abstract: To enhance the production of terpenoid indole alkaloids in Rauwolfia serpentina, Catharanthus tryptophan decarboxylase (Crtdc) gene was over-expressed in transgenic hairy root cultures using Agrobacterium rhizogenes-mediated transformation. Among six transgenic hairy root lines, line RT4 accumulated the highest alkaloid content, with 0.1202 % dry weight (DW) reserpine and 0.0064 % DW ajmalicine, after 10 weeks of culture. Whereas, wild-type roots accumulated 0.0596 ± 0.003 % DW reserpine and 0.0011 ± 0.001 % DW ajmalicine. Transgenic hairy root line RT7 produced the lowest alkaloid content (reserpine: 0.0896 ± 0.002 % DW; ajmalicine: 0.002 ± 0.0 % DW). On the basis of alkaloid content the six hairy root lines were grouped as RT4/RT2 > RT3/RT5 > RT7/RT8. Analysis of gene expression profile indicated that Crtdc was expressed at a higher level in transgenic lines, which could be correlated with enhanced metabolite accumulation in roots. This study confirms that over-expression of Crtdc is a superlative method to improve the biosynthetic potential of Rauwolfia hairy root cultures. Enhanced reserpine and ajmalicine production can serve as an alternative choice to provide resources for relative pharmaceutical industries.
32 citations