scispace - formally typeset
Topic

Induced pluripotent stem cell

About: Induced pluripotent stem cell is a(n) research topic. Over the lifetime, 27779 publication(s) have been published within this topic receiving 1147118 citation(s). The topic is also known as: iPS cell & iPSc.

...read more

Papers
  More

Open accessJournal ArticleDOI: 10.1016/J.CELL.2006.07.024
Kazutoshi Takahashi1, Shinya Yamanaka1Institutions (1)
25 Aug 2006-Cell
Abstract: Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.

...read more

Topics: KOSR (74%), Induced pluripotent stem cell (72%), Stem cell (70%) ...read more

21,746 Citations


Open accessJournal ArticleDOI: 10.1016/J.CELL.2007.11.019
Kazutoshi Takahashi1, Koji Tanabe1, Mari Ohnuki1, Megumi Narita1  +3 moreInstitutions (1)
30 Nov 2007-Cell
Abstract: SUMMARY Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generationofiPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

...read more

Topics: Induced pluripotent stem cell (72%), Embryonic stem cell (71%), Stem cell (70%) ...read more

16,624 Citations


Journal ArticleDOI: 10.1126/SCIENCE.1151526
21 Dec 2007-Science
Abstract: Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.

...read more

Topics: Induced pluripotent stem cell (70%), Embryonic stem cell (70%), Embryoid body (70%) ...read more

9,307 Citations


Journal ArticleDOI: 10.1038/292154A0
Martin J. Evans1, Matthew H. Kaufman1Institutions (1)
09 Jul 1981-Nature
Abstract: Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.

...read more

Topics: Embryoid body (54%), Cell culture (54%), Cell potency (54%) ...read more

7,763 Citations


Open accessJournal ArticleDOI: 10.1038/NATURE00870
04 Jul 2002-Nature
Abstract: We report here that cells co-purifying with mesenchymal stem cells--termed here multipotent adult progenitor cells or MAPCs--differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro. When injected into an early blastocyst, single MAPCs contribute to most, if not all, somatic cell types. On transplantation into a non-irradiated host, MAPCs engraft and differentiate to the haematopoietic lineage, in addition to the epithelium of liver, lung and gut. Engraftment in the haematopoietic system as well as the gastrointestinal tract is increased when MAPCs are transplanted in a minimally irradiated host. As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases.

...read more

  • Figure 5 Immunofluorescence staining of individual organs from a 45% chimaeric mouse. a, b, Brain sections of cortex (a) and hippocampus (b) stained with anti-b-gal-Cy3,
    Figure 5 Immunofluorescence staining of individual organs from a 45% chimaeric mouse. a, b, Brain sections of cortex (a) and hippocampus (b) stained with anti-b-gal-Cy3,
  • Figure 1 Characteristics of mMAPCs. Mouse BMMNCs were plated with EGF, PDFG-BB, and LIF on FN-coated plates. After 3–4 weeks, bead-selected CD452/TER1192 cells
    Figure 1 Characteristics of mMAPCs. Mouse BMMNCs were plated with EGF, PDFG-BB, and LIF on FN-coated plates. After 3–4 weeks, bead-selected CD452/TER1192 cells
  • Figure 6 Engraftment and in vivo differentiation of mMAPCs. Tissue sections of a NOD/ SCID mouse (Table 2: animal 12) 11 weeks after infusion of 106 ROSA26 MAPCs.
    Figure 6 Engraftment and in vivo differentiation of mMAPCs. Tissue sections of a NOD/ SCID mouse (Table 2: animal 12) 11 weeks after infusion of 106 ROSA26 MAPCs.
  • Figure 2 Single cell origin of mMAPC and rMAPC cultures initiated from ten cells per well, and their differentiated progeny (see also Supplementary Information Table 2). Mouse or rat BMMNCs, transduced with an MSCV-eGFP retrovirus49, were depleted of CD45þ TER119þ cells, and eGFPþ cells were replated at ten cells per well and expanded for
    Figure 2 Single cell origin of mMAPC and rMAPC cultures initiated from ten cells per well, and their differentiated progeny (see also Supplementary Information Table 2). Mouse or rat BMMNCs, transduced with an MSCV-eGFP retrovirus49, were depleted of CD45þ TER119þ cells, and eGFPþ cells were replated at ten cells per well and expanded for
  • Table 1 Degree of chimaerism after ROSA26 MAPC injection
    Table 1 Degree of chimaerism after ROSA26 MAPC injection
  • + 2

5,349 Citations


Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202261
20212,380
20202,315
20192,223
20181,990
20172,263

Top Attributes

Show by:

Topic's top 5 most impactful authors

Shinya Yamanaka

231 papers, 82.6K citations

Joseph C. Wu

172 papers, 12.7K citations

Hideyuki Okano

135 papers, 5.8K citations

Juan Carlos Izpisua Belmonte

121 papers, 11.5K citations

George Q. Daley

114 papers, 22.1K citations

Network Information
Related Topics (5)
Embryonic stem cell

35.1K papers, 1.9M citations

94% related
Adult stem cell

19.4K papers, 1.3M citations

94% related
Stem cell

129.1K papers, 5.9M citations

94% related
Progenitor cell

50.8K papers, 2.5M citations

93% related
Cellular differentiation

90.9K papers, 6M citations

92% related