Showing papers on "Isopimpinellin published in 1986"
TL;DR: The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products, xanthotoxin and bcrgapten, which were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells.
Abstract: Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean pathogen, Phytophthora megasperma f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-ʟ-methionine: xanthotoxol and S-adenosyl-ʟ-m ethionine: bergaptol O-methyltransferases. The products, xanthotoxin and bcrgapten, were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells. The latter enzyme also catalyzed the methylation of 5-hydroxyxanthotoxin to isopimpinellin, another major coumarin derivative in this system. The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products. No activity was detectable in untreated cells. Activity increases of the O-methyltransferases occurred a few hours later than those of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase.
82 citations
TL;DR: The aerial parts of Ruta chalepensis yielded the alkaloids kokusaginine, skimmianine, arborinine, γ-fagarine, graveoline and the new alkaloid 3′-hydroxygraveoline as mentioned in this paper.
41 citations
TL;DR: Oxypeucedanin, not previously reported from parsley, was found to be the major component (70-100 ppm wet weight), although only moderately photoactive, its high concentration in parsley may be partially responsible for contact photodermatitis.
Abstract: Fresh parsley leaves and roots were analyzed by HPLC and photobiological assay for photoactive furocoumarins. Oxypeucedanin ( 7), not previously reported from parsley, was found to be the major component (70-100 ppm wet weight). Although only moderately photoactive, its high concentration in parsley may be partially responsible for contact photodermatitis. Other photoactive compounds, namely 5-MOP ( 2), 8-MOP ( 3), psoralen ( 1), isopimpinellin ( 4) and imperatorin ( 5) were also present and quantified.
36 citations
TL;DR: To some degree there was a correlation between the amount of cross‐linking and the relative potency of anti‐MCMV infectivity, but other factors evidently contribute to the phototoxic effect of these compounds.
Abstract: — Six different photosensitizers were compared for their ability to form cross-links in murine cellular DNA and murine cytomegalovirus DNA, in the presence of long wave UV radiation. The viral DNA was in the form of free DNA or intact virions. The compounds consisted of the linear furanocoumarins 8-methoxypsoralen (8-MOP) and isopimpinellin; the angular furanocoumarin, angelicin; the two furanochromones, visnagin and khellin; and the β-carboline alkaloid, harmine. Cross-linking was assessed by alkaline agarose gel electrophoresis and hydroxyapatite chromatography. 8-MOP produced extensive cross-linking (as expected), as did isopimpinellin. Visnagin produced less cross-linking, such that not all DNA molecules were affected at the concentrations used. Khellin, angelicin and harmine produced no detectable cross-linking. The same result was obtained for DNA which was treated in situ in the virion. To some degree there was a correlation between the amount of cross-linking and the relative potency of anti-MCMV infectivity. But other factors evidently contribute to the phototoxic effect of these compounds.
25 citations
Journal Article•
TL;DR: Eight coumarins were isolated from the root of ZOU-MA-QIN (Heracleum moellendorffii Hance Var. Paucivitatum) and a new coumarin named mollendorffiline was determined by X-ray crystallographic analysis.
Abstract: Eight coumarins were isolated from the root of ZOU-MA-QIN (Heracleum moellendorffii Hance Var Paucivitatum) They were identified as isobergapten(Ⅰ), pimpinellin(Ⅱ), osthol(Ⅲ), bergapten (Ⅳ), isopimpinellin(Ⅴ), sphondin(Ⅵ), 4'-hydroxy-columbianetin(Ⅶ), by spectroscopic analysis (UV IRMS ~1H NMR) (Ⅷ) is a new coumarin named moellendorffiline, C_26H_20O_10,m p 244-246℃, its structure was determined by X-ray crystallographic analysisThe space group of the crystal is monoclinic P2_1/c, with four molecules in aUnit cell The cell dimension are a=15875, b=10260, c=14 602, β=10724
Six coumarins (Ⅰ～Ⅵ) were isolated from the leaves and seeds of the same plant
3 citations