Showing papers on "Isopimpinellin published in 2014"

Phenylpropanoids and furanocoumarins as antibacterial and antimalarial constituents of the Bhutanese medicinal plant Pleurospermum amabile.

Abstract: With the objective of determining safety and verifying the traditional uses of the Bhutanese medicinal plant, Pleurospermum amabile Craib & W. W. Smith, we investigated its crude extracts and the isolated phytochemicals for their biological activities. Four phenylpropanoids [(E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4)] and six furanocoumarins [psoralen (5), bergapten (6), isoimperatorin (7), isopimpinellin (8), oxypeucedanin hydrate (9) and oxypeucedanin methanolate (10)] were isolated from this plant. Among the test samples, compound 10 showed weak antibacterial activity against Bacillus subtilis and best antimalarial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). None of the test samples showed cytotoxicity. This study generated scientific data that support the traditional medical uses of the plant.

Effect of light on contents of coumarin compounds in shoots of Ruta graveolens L. cultivated in vitro

Abstract: Shoots of Ruta graveolens L. ( Rutaceae ) were cultivated in stationary liquid culture under different light conditions: constant artificial light (900 lx), darkness, constant artificial light (900 ix) following irradiation with UV-C light. The contents of five furanocoumarins: psoralen, bergapten, xanthotoxin, isopimpinellin and imperatorin, as well as biogenetic precursor of these metabolites, umbelliferone, were determined by HPLC method in shoots cultivated in vitro and in overground parts of plants growing in open air. It was shown that light conditions, tested in these experiments, significantly influenced contents of the metabolites in shoots cultivated in in vitro culture. Total content of the coumarin compounds in shoots cultivated under constant artificial light (900 lx) was equal or higher than in plants growing under natural conditions. Therefore, it is suggested that stationary liquid shoot culture of R. graveolens . can be an alternative source for obtaining biologically active furanocoumarins.

Foeniculum vulgare Mill. A Medicinal Herb

Abstract: Foeniculum vulgare .Mill. commonly known as Fennel belonging to the family Apiaceae , is a small, erect and aromatic herb. A number of chemical constituents and various therapeutic effects of this herb have been reported by different workers. Extensive investigations have been carried out on different parts of herb and as a consequence, varied classes of compoundsfatty acids, hydrocarbons and sterols, Fu­rocoumarins; (imperatorin, psoralen, ber­gapten, xanthotoxin and isopimpinellin), Flavonoids; (isorhamnetin 3-O-α-rhamnoside, quercetin and kaempferol) and quercetin; (3-O-rutinoside, kaempferol 3-O-rutinoside and quercetin 3-O- β-glucoside) have been isolated . So, it has been used in ethnomedicine to exploit its medicinal properties including antioxidant, anti-inflammatory, anticancer, antifungal, antibacterial, antinociceptive, anti-inflammatory and antiviral activities. The presented review summarizes the information about the ethnopharmacology, toxicity, phytochemistry and biological activity of F. vulgare .

Coumarins and alkaloids in shoot culture of Ruta graveolens L.

Abstract: A shoot culture of Ruta graveolens L. ( Rutaceae ) was maintained in the stationary liquid phase. From the cultured shoots seven compounds were isolated and identified as psoralen, bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins), rutamarin (linear dihydrofuranocoumarin), kokusaginine and skimmianine (furanoquinoline alkaloids) by spectral methods. The compounds are known as secondary metabolites of the intact plant, as well as its cell and tissue cultures.

Matrix solid-phase dispersion versus ultrasound assisted extraction with solid-phase extraction in the HPLC analysis of furanocoumarins from fruits of Archangelica officinalis Hoffm

Abstract: In this study an efficient matrix solid-phase dispersion (MSPD) method for the simultaneous HPLC analysis of furanocoumarins from fruits of Archangelica officinalis Hoffm. was performed. Herbal samples were prepared by an optimized MSPD procedure using C18 as sorbent. The analysis was performed by high performance liquid chromatography with diode array detector (HPLC-DAD). The efficiency of the MSPD method was also compared with ultrasound assisted extraction with solid-phase extraction (USAE with SPE). The MSPD extracted furanocoumarins (isoimperatorin, imperatorin, bergapten, isopimpinellin, xanthotoxin, umbelliferone and xanthotoxol) from Archangelica officinalis with satisfactory recoveries ranging from 91.43% to 96.07% and relative standard deviations lower than 4.34%. The detection limit of various furanocoumarins was found to be in the range of 0.37 mg mL-1 for xanthotoxol to 10.82 mg mL-1 for imperatorin. The results presented in the paper reveal that MSPD is efficient, fast, simple and easy to perform method suitable for the isolation of furanocoumarins from herbs.

[Coumarins from Leonurus japonicus and their anti-platelet aggregative activity].

Abstract: Chemical constituents of Leonurus japonicus were isolated and purified by a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, MCI, and Rp C18. Structures of the isolates were determined by spectroscopic analysis as 10 coumarins: bergapten (1), xanthotoxin (2), isopimpinellin (3), isogosferal (4), imperatorin (5), meransin hydrate(6), isomeranzin(7), murrayone(8) , auraptenol(9), and osthol(10). In addition to compound 9, the others were isolated from the genus Leonurus for the first time. In the in vitro assay, compounds 4 and 8 significantly inhibited the abnormal increase of platelet aggregation induced by ADP.

Chemical constituents of Angelica nitida roots

Abstract: OBJECTIVE To investigate the chemical constituents in the roots of Angelica nitida. METHODS The chemical constituents were isolated by silica gel,their structures were identified by spectral analysis. RESULTS Nine compounds were isolated and identified as isoimperatorin(I), imperatorin(II), cnidilin(III), beta-sitosterol(IV), isopimpinellin(V), phellopterin(VI), neobyakangelicol(VII), (3S)-2,2-dimethyl-3,5-dihydroxy-8-hydroxylmethyl-3,4-dihydro-2H,6H-benzo[1,2-b: 5,4-b'] dipyran-6-one(VIll) and byakangelicin (IX). CONCLUSION All the compounds are isolated from the plant for the first time.

Chemical constituents in root bark of Changium smyrnioides

Abstract: Objective To study the chemical constituents from the root bark of Changium smyrnioides. Methods Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, and recycling preparative HPLC from the ethanol extract in the root bark of C. smyrnioides, and their structures were elucidated by the physicochemical characteristics and spectral analyses. Results Fifteen chemical constituents were obtained and identified as imperatorin(1), phellopterin(2), xanthotoxol(3), 5-hydroxy-8-methoxy-psoralen(4), vanillic acid(5), alloimperatorin(6), psoralen(7), bergapten(8), 8-O-β-D-glucopyranosyl-5-methoxylpsoralen(9), isopimpinellin(10), caffeic acid(11), aurantiamide acetate(12), vaginatin(13), β-sitosterol(14), and succinic acid(15). Conclusion Compounds 6—13 are isolated from the plants in Changium Wolff for the first time.

Study on chemical constituents of Toddalia asiatica

Abstract: OBJECTIVE To investigate the chemical constituents of Toddalia asiatica. METHODS The compounds were isolated and pu- rified by column chromatography and their structures were identified on the basis of physicochemical properties and spectral data analysis. RESULTS Sixteen compounds were isolated and identified as zanthocadinanine A(1), pimpinellin(2), isopimpinellin(3), phellopterin (4), armottianamide(5), chelerythrine(6), nitidine(7), chlorogenic acid (8), toddalolactone (9), protopine(10), skimmianine(11), dictamine(12), toddalenone(13), beta-sitosterol(14), bergapten(15) and 8-hydroxy-6-methoxycoumarin(16). CONCLUSION Compound 1 and 16 are isolated from Toddalia genus for the first time.

Isolation of furanocoumarins from Pastinaca sativa L. callus culture

Abstract: Four furanocoumarins: bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins) and sphondin (angular furanocoumarin) were isolated for the first time from callus tissues of Pastinaca sativa L.( Apiaceae ) cultured in vitro on solid medium. The compounds were identified using spectral methods. They are well-known secondary metabolites of the intact plant. This is the first report on the isolation of sphondin from in vitro plant cultures.

Application of isopimpinellin in preparation of antitumor drugs and anticancer reversal agents

Abstract: The invention relates to an application of isopimpinellin in the preparation of antitumor drugs and anticancer reversal agents. The invention aims at the pathological and physiological characteristics of tumors, can change the pathological changes of the tumors, and also can enhance the drug concentration of other chemotherapeutic drugs in the tissues and cells of the tumors in order to realize synergistic attenuation effects of the chemotherapeutic drugs. The invention discloses spectroscopic analysis structure confirmation of highly pure isopimpinellin, a preparation method of isopimpinellin, stability study, acute toxicity studies, a medicinal composition containing isopimpinellin, and a new drug use of the above new natural product. Isopimpinellin with the purity of above 98% is obtained through the steps of extracting Fructus Cnidii with ethanol, carrying out macroporous resin decolorization enrichment, and carrying out resin separation and purification. Researches on the pharmacological activity of isopimpinellin show that isopimpinellin has obvious lung cancer, liver cancer and cervical cancer resistances, and has a leukocytogenic effect on leucocytopenia. Isopimpinellin is expected to be developed into low-toxicity and high-efficiency new drugs and anticancer reversal agents used for preventing and treating natural tumors and having pharmacological efficacies, namely drugs for the auxiliary treatment of the tumors.

High performance liquid chromatography (HPLC) method for measuring content of main components in fructus cnidii

Abstract: The invention relates to a method for measuring the content of xanthotoxin, isopimpinellin, imperatorin and cnidium lactone in fructus cnidii medicine. The method comprises the steps of respectively preparing xanthotoxin reference substance solution, isopimpinellin reference substance solution, imperatorin reference substance solution and cnidium lactone reference substance solution; preparing fructus cnidii extract test solution; carrying out gradient elution, wherein the chromatographic column is Agilent-C18 (250mm*4.6mm, 5 micrometers), and mobile phase is acetonitrile-phosphate buffer salt solution (pH=3.5); respectively measuring the reference substance solution and the test solution at the wave lengths being 250nm and 324nm, and recording the chromatogram, wherein the detection temperature is 40 DEG C, the flow velocity is 1.0ml/ min, and the detector is an ultraviolet detector. The method has the technical characteristics of being high in detection sensitivity, accurate, reliable, good in separation effect, simple, convenient and fast in analysis, etc.