Showing papers on "Isopimpinellin published in 2018"
TL;DR: The developed method ensured good repeatability of corrected peak areas and migration times and good linearity of the relationship between the peak corrected area and the concentration of the compounds was observed and was successfully applied in the quantitative analysis of two different types of samples.
18 citations
TL;DR: A synergistic assay results demonstrated that oxoglaucine and isopimpinellin showed significant synergistic efficacy with GFT, indicating that these components may act on other membrane receptors.
17 citations
TL;DR: Isopimpinellin and karanjin showed the most potent inhibition of CYP1A1 in human cells, and molecular docking and molecular dynamic simulations with CYP isoforms rationalize the observed trends in the potency and selectivity.
Abstract: CYP1A1 is thought to mediate carcinogenesis in oral, lung and epithelial cancers. In order to identify a CYP1A1 inhibitor from an edible plant, 394 natural products in the IIIM's natural product repository were screened, at 10 μM concentration, using CYP1A1-Sacchrosomes™ (i.e. microsomal enzyme isolated from recombinant baker's yeast). Twenty-seven natural products were identified that inhibited 40–97% of CYP1A1's 7-ethoxyresorufin-O-deethylase activity. The IC50 values of the ‘hits’, belonging to different chemical scaffolds, were determined. Their selectivity was studied against a panel of 8 CYP-Sacchrosomes™. In order to assess cellular efficacy, the ‘hits’ were screened for their capability to inhibit CYP enzymes expressed within live recombinant human embryonic kidney (HEK293) cells from plasmids encoding specific CYP genes (1A2, 1B1, 2C9, 2C19, 2D6, 3A4). Isopimpinellin (IN-475; IC50, 20 nM) and karanjin (IN-195; IC50, 30 nM) showed the most potent inhibition of CYP1A1 in human cells. Isopimpinellin is found in celery, parsnip, fruits and in the rind and pulp of limes whereas different parts of the Indian beech tree, which contain karanjin, have been used in traditional medicine. Both isopimpinellin and karanjin negate the cellular toxicity of CYP1A1-mediated benzo[a]pyrene. Molecular docking and molecular dynamic simulations with CYP isoforms rationalize the observed trends in the potency and selectivity of isopimpinellin and karanjin.
14 citations
TL;DR: In this paper, a long-term experiment was carried out to simulate storage of celery (Apium graveolens) under industrial (air-conditioned store)/household (common cellar) conditions, where several celery cultivars from both organic and/or conventional production were monitored for furanocoumarins levels for 16-26 weeks.
Abstract: Long term experiments, which simulated storage of celery (Apium graveolens) under industrial (air-conditioned store)/household (common cellar) conditions, were carried out. Several celery cultivars from both organic and/or conventional production were monitored for furanocoumarins levels for 16-26 weeks. The increase of furanocoumarin concentrations during the storage of all the tested celery cultivars was observed, nevertheless, the extent of toxicants accumulation differed among tested cultivars. The changes of furanocoumarin levels occurring during processing of vegetables were studied, too. Levels of both linear (psoralen, bergapten, xanthotoxin, trioxsalen, isopimpinellin) and angular (angelicin, sphondin, isobergapten) furanocoumarins in tested vegetable were determined by validated GC/MS (SIM) method. The detection limits (LODs) obtained by this analytical method were around 0.003 mg/kg.
2 citations