Topic
Isopimpinellin
About: Isopimpinellin is a research topic. Over the lifetime, 227 publications have been published within this topic receiving 4740 citations. The topic is also known as: 5,8-Dimethoxypsoralen & 5,8-Dimethoxypsoralene.
Papers published on a yearly basis
Papers
More filters
TL;DR: Using media-modified assays, furocoumarins extracted from four varieties of fresh and freeze-dried parsley leaves inhibited a DNA repair-deficient Escherichia coli in a photobiological assay and the human pathogens E. coli O157:H7 and Listeria monocytogenes, the spoilage microorganism Erwinia carotovora, and Listersia innocua were inhibited.
84 citations
TL;DR: The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products, xanthotoxin and bcrgapten, which were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells.
Abstract: Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean pathogen, Phytophthora megasperma f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-ʟ-methionine: xanthotoxol and S-adenosyl-ʟ-m ethionine: bergaptol O-methyltransferases. The products, xanthotoxin and bcrgapten, were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells. The latter enzyme also catalyzed the methylation of 5-hydroxyxanthotoxin to isopimpinellin, another major coumarin derivative in this system. The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products. No activity was detectable in untreated cells. Activity increases of the O-methyltransferases occurred a few hours later than those of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase.
82 citations
TL;DR: A high-speed counter-current chromatography method for separation and purification of coumarins from Cnidium monnieri (L.) Cusson was developed by using stepwise elution with a pair of two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water at volume ratios of 5.5:3.5.
81 citations
TL;DR: It is indicated that osthol and imperatorin can induce apoptosis in HL-60 cells and are cytotoxic marker substances in the fruits of Cnidium monnieri.
Abstract: Cnidii monnieri Fructus [CmF; Cnidium monnieri (L.) Cusson] is used as a tonic agent in traditional Chinese medicine. In a previous Chinese herb-cytotoxicity screening test, the ethanol extract of CmF exhibited strong effects on human leukemia (HL-60), cervical carcinoma (HeLa) and colorectal carcinoma (CoLo 205) cells. Then, the CmF extract was subjected to silica gel column chromatography and recrystallization to give five coumarins: osthol, imperatorin, bergapten, isopimpinellin, and xanthotoxin. Among these compounds, osthol showed the strongest cytotoxic activity on tumor cell lines. The structure-activity relationship established from the results indicated that the prenyl group has an important role in the cytotoxic effects. However, imperatorin showed the highest sensitivity to HL-60 cells and the least cytotoxicity to normal PBMCs. Osthol and imperatorin both caused apoptotic bodies, DNA fragmentation, and enhanced PARP degradation in HL-60 cells by biochemical analysis. These results indicate that osthol and imperatorin can induce apoptosis in HL-60 cells. Therefore, osthol and imperatorin are cytotoxic marker substances in the fruits of Cnidium monnieri.
77 citations
TL;DR: In this article, the potential of R. graveolens for the production of furanocoumarins (Psoralen, xanthotoxin, bergapten, isopimpinellin) was evaluated.
75 citations