Showing papers on "Isovitexin published in 2018"
TL;DR: Six flavonoids were isolated and fully identified from the yellow grain rice mutant, and the levels were significantly higher than wild-type, with isoorientin particularly abundant in mutant embryo.
31 citations
TL;DR: The data suggest that isovitexin has the potential to be an anti-infective drug against S. aureus via the inhibition of sortase activity.
Abstract: Staphylococcus aureus (S. aureus) causes a broad variety of diseases. The spread of multidrugresistant S. aureus highlights the need to develop new ways to combat S. aureus infections. Sortase A (SrtA) can anchor proteins containing LPXTG binding motifs to the bacteria surface and plays a key role in S. aureus infections, making it a promising antivirulence target. In the present study, we used aSrtA activity inhibition assay to discover that isovitexin, a Chinese herbal product, can inhibit SrtA activity with an IC50 of 28.98 μg/ml. Using a fibrinogenbinding assay and a biofilm formation assay, we indirectly proved the SrtA inhibitory activity of isovitexin. Additionally, isovitexin treatment decreased the amount of staphylococcal protein A (SpA) on the surface of the cells. These data suggest that isovitexin has the potential to be an anti-infective drug against S. aureus via the inhibition of sortase activity.
28 citations
TL;DR: Further isolation and structural elucidation of newly described acetylated flavonoids are needed in order to determine their relevance in the beneficial properties of the plant.
24 citations
TL;DR: The results showed that the severity of liver injury was attenuated by treatment of isovitexin, as confirmed by the decreased liver histopathologic changes, as well as serum AST and ALT levels.
24 citations
TL;DR: A specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C. communis Linn, which included orientin, iso-orientin, vitex in, isovitexin, rutin, apigenin, protocatechuate, vanillic acid, caffeic acid and ferulic acid.
Abstract: This study reported the identification and determination of the main components of Commelina communis Linn. A total of 62 compounds were identified in C. communis Linn. extract, which included 29 flavonoids and flavonoid glycosides, 17 phenolic acids, 4 alkaloids, 1 pyrimidine alkaloids, 3 sterols and 8 fatty acids and others by ultra high performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Moreover, a specific, simple, rapid and sensitive high performance liquid chromatography with tandem mass spectrometry method was developed and validated for determination of 13 components of C. communis Linn., which included orientin, iso-orientin, vitexin, isovitexin, rutin, apigenin, protocatechuate, vanillic acid, caffeic acid, ferulic acid, luteolin, quercetin and isorhamnetin. All calibration curves showed good linearity (r ≥ 0.9991) within the test range. The intra- and inter-day precisions (relative standard deviation%, RSD%) were within 1.04 and 0.92%, and the recoveries ranged from 98.64 to 100.8%. These results may contribute to the further study and quality control for C. communis Linn.
23 citations
TL;DR: Training system presented no effects on TP, PA, TF and antioxidant activity, whereas rainy season year 2 increased FRAP values, TF, orientin, isovitexin and hesperetin content, which could be associate to climate stressful condition leading to higher levels of these compounds.
23 citations
TL;DR: These findings provide the first evidence that Isovitexin and 6‐C‐glycoside‐Diosmetin could exert their memory‐enhancing and anxiolytic‐like effects via GABAA receptor modulation, which likely occurs via their benzodiazepine‐binding site.
23 citations
TL;DR: The phenylpropanoid profiles of the first and third leaf of barley seedlings in Scarlett and four members of the S42IL-population were obtained by LC-MS and revealed a change in the glycosylation pattern of the flavone-6-C-glucoside isovitexin in the elite cultivar Scarlett.
15 citations
TL;DR: The results suggest that vitexin is more potent and stable against α-amylase enzyme, thus it could develop as a therapeutic drug for the treatment of diabetes.
15 citations
TL;DR: In this article, the in vitro hepatoprotective activity of the methanol extract of Desmodium uncinatum, its sub-fractions, the major isolated compounds and some of their semi-synthetic derivatives in order to study structure activity relationships was investigated.
Abstract: Lipid peroxidation plays a pivotal role in the pathogenicity and maintenance of hepatitis. Thus, substances protecting hepatocyte membranes from lipid peroxidation are of great importance in the management of hepatotoxicity and hepatitis. The present work deals with the in vitro hepatoprotective activity of the methanol extract of Desmodium uncinatum, its sub-fractions, the major isolated compounds and some of their semi-synthetic derivatives in order to study structure activity relationships. Using hydrogen peroxide (H2O2)-induced lipid peroxidation of hepatocyte membranes as a model, the hepatoprotective-guided phytochemical survey of the methanol extract of aerial parts of D. uncinatum was carried out by successive column chromatography. One of the most active compounds (Isovitexin) was chemically transformed to yield new semi-synthetic. The identification of isolated and semi-synthetic compounds was performed using NMR techniques, mass spectrometry and by comparison of their data with those reported in the literature. The n-butanol fraction was the most effective (IC50: 22.9 μg/mL) compared to the crude methanol extract (IC50: 43.6 μg/mL) and other fractions. The n-butanol sub-fractions FA (containing non-phenolic compounds) and FB (mainly containing phenolic compounds) exhibited respective IC50 of 14.36 and 128.2 μg/ml. Purification of FA yielded 3-O-β-D-glucopyranosyl-β-sitosterol (1), 3-O-β-D- 2-acetyl-amino-2-deoxyglucopyranoxyloleanoic acid (2), (2S, 3S, 4R, 7R, 8Z)-1-O-β-D-glucopyranosyl-2-[(R)-2'-hydroxyarachidoylamino]-docosan-8-ene-3,4,7-triol (4), spiraeamide (5), mannitol (6), while FB afforded essentially three C-glycosylflavonoids namely isovitexin (7), vitexin (8) and vicenin-3 (9). Chemical transformations (methylation, allylation and prenylation) of isovitexin afforded five new semi-synthetic derivatives: 4',5,7-O- trimethyli-sovitexin (10), 4'-O-allylisovitexin (11), 4',7-O-diallylisovitexin (12), 4'-O-prenylisovitexin (13) and 8-C-prenyl-4',7-O-diprenylisovitexin (14). The screening of these derivatives revealed that allylation did not significantly affect the hepatoprotective activity while methylation, prenylation, number and position of sugar moieties on the A ring of flavonoids significantly reduced it. Results demonstrated that the n-butanol fraction obtained from the methanol extract of Desmdium uncinatum may possess hepatoprotective activity due to its content in C-glycosylflavonoids and cerebrosides. Hydroxyl groups in C-glycosylflavonoids are important for their lipid peroxidation inhibitory activity.
8 citations
TL;DR: A liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of six bioactive constituents including vitexin, orientin, isoorientin, 2″-O-β-d-xylopyranosyl isoraientin and 6-C-L-α-arabipyrin as the internal standard as mentioned in this paper.
Abstract: A liquid chromatography-tandem mass spectrometry method was developed and validated for simultaneous determination of six bioactive constituents including vitexin, orientin, isoorientin, 2″-O-β-d-xylopyranosyl isoorientin, 2″-O-β-d-xylopyranosyl isovitexin, and 6-C-L-α-arabipyranosyl vitexin in rats’ various tissues using isoquercitrin as the internal standard. Biological samples were pretreated by protein precipitation with acetonitrile. Chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and 0.1% aqueous formic acid. All analytes and internal standard were quantitated through electrospray ionization in negative ion selected reaction monitoring mode. The mass transitions were as follows: m/z 431 → 311 for vitexin, m/z 447 → 327 for orientin or isoorientin, m/z 579 → 459 for 2″-O-β-d-xylopyranosyl isoorientin, m/z 563 → 293 for 2″-O-β-d-xylopyranosyl isovitexin, m/z 563 → 353 for 6-C-L-α-arabipyranosyl vitexin, and m/z 463 → 300 for the internal standard, respectively. The lower limits of quantification for the six analytes in different tissue homogenates were 0.8–2.2 ng/ml. The validated assay was successfully applied to a tissue distribution study of the six components in rats after intravenous administration of total flavonoids of Scorzonera austriaca Willd; Asteraceae. The results of the tissue distribution study showed that the high concentrations of six components were mainly in the kidney.
TL;DR: In this paper, a microwave assisted hydrodistillation (MAHD) was used for the extraction of vitexin and isovitexins from Ficus deltoidea.
01 Dec 2018
TL;DR: In this paper, the performance of aqueous or water extraction of Mas Cotek leaves for vitexin and isovitic acid compounds using three regiments of temperatures (50, 70 and 100°C) in sample-to-water ratios of 1:10, 1:20 and 1:30g/mL during 8 hours of extraction.
Abstract: Mas Cotek or Ficus deltoidea is regarded as one of the most precious herbal plants in Malaysia due to its content of beneficial active compounds and appreciation as a traditional remedy. This research investigated performance of aqueous or water extraction of dried Mas Cotek leaves for vitexin and isovitexin compounds using three regiments of temperatures (50, 70 and 100°C) in sample-to-water ratios of 1:10, 1:20 and 1:30g/mL during 8 hours of extraction. The optimum yield recorded for vitexin compound was 0.463 ± 0.045 (%w/w) from; 1:30 g/mL sampleto-water ratio, 50°C temperature at 4th hour of extraction. While for isovitexin compound, the optimum value recorded was 0.136 ± 0.015 (%w/w) from; 1:20 (g/mL) sample-to-water ratio, 50°C temperature at 5th hour of extraction. The morphological characterization of the
extracted dried leaves particles performed using FE-SEM showed an intact and less damaged surface of extracted sample after 8 hours of extraction. The experimental values under best conditions were consistent with the predicted values, which suggested that aqueous extraction of Mas Cotek leaves is a good platform to serve as baseline study for more advanced extraction techniques.
TL;DR: This work focused on the stability in gastrointestinal conditions and biotransformation by intestinal microflora in the colon of D‐pinitol, vitexin and the flavonoid fraction of the decoction, as a first step in unravelling its behaviour in the human body.
Abstract: Objectives The isolation and identification of the flavonoids present in a decoction of Desmodium adscendens was performed. In view of the oral use of the decoction, this work focused on the stability in gastrointestinal conditions and biotransformation by intestinal microflora in the colon of D-pinitol, vitexin and the flavonoid fraction of the decoction, as a first step in unravelling its behaviour in the human body. Methods The freeze-dried decoction was first subjected to column chromatography. Subsequently an enriched flavonoid fraction, was separated by repeated semi-preparative high-performance liquid chromatography (HPLC) or by HPLC-SPE. The isolated compounds were elucidated by NMR. Biotransformation experiments were carried in an in vitro gastrointestinal dialysis model. Key findings The major flavonoids of a decoction of D. adscendens were characterized as vicenin-2, isoschaftoside, schaftoside, 2″-O-xylosylvitexin, 2″-O-pentosyl-C-hexosyl apigenin and a O-hexosyl-C-hexosyl apigenin, tentatively identified as 2″-O-glucosyl-vitexin. During their passage in the gastrointestinal dialysis model, vitexin and C-glycosides thereof were found to be stable. Only the O-glycosidic bonds of O-glycosides of vitexin or isovitexin were hydrolysed during the colonic phase. Conclusions A D. adscendens decoction was found to be rich in vitexin and isovitexin glycosides from which vitexin and the C-glycosides thereof were found to be stable in the simulated gastrointestinal tract.
TL;DR: The secondary metabolites of the aerial parts of Zornia brasiliensis Vogel, Fabaceae, and the biological activity of one of these secondary metabolites were characterized were characterized in this study.
Abstract: The secondary metabolites of the aerial parts of Zornia brasiliensis Vogel, Fabaceae, and the biological activity of one of these secondary metabolites were characterized in this study. A phytochemical investigation was performed using chromatographic techniques including analytical and preparative reverse-phase HPLC column sequences, which resulted in the isolation of fourteen compounds: one previously undescribed C-glycosylated dihydrochalcone (zornioside), one cyclitol (D-pinitol), one glycosylated megastigmane (roseoside) and eleven phenolic compounds: 7-methoxyflavanone, 7, 4′-dimethoxyisoflavone, medicarpin, 2′-4′-dihydroxychalcone, onionin, isoorientin-3′-O-methyl ether, isovitexin, glycosylated (Z)-O-coumaric acid, glycosylated (E)-O-coumaric acid, dihydromelilotoside, and isoorientin. The structures of the isolated compounds were determined based on 1D and 2D-NMR, HRESIMS, IR and CD spectroscopic analyses. The cytotoxic activity of zornoside was assessed against tumor cell lines (MCF-7, HCC1954, T-47D, 4T1, HL60), and a non-tumor cell line (RAW264.7) using MTT assay. The compound zornioside was selectively cytotoxic for HL60 leukemia cells (IC50: 37.26 μM).
TL;DR: The ethyl acetate fraction and subfraction elicited the best anti-inflammatory activity and inhibited acetic acid-induced pain at the doses of 100mg/kg and 200 mg/kg, respectively, which was better than the results observed for diclofenac at similar doses.
Abstract: Background: Anthocleista nobilis (Loganiaceae) is used by Mbano people of Imo State, Nigeria, for the treatment of various ailments Objective: The aim of this study is to evaluate the antioxidant, anti-inflammatory, and analgesic properties of the methanol extract, fractions, and subfractions of A. nobilis. Materials and Methods: The powdered stem bark was extracted with methanol and sequentially fractionated into n-hexane, ethyl acetate, and butanol fractions. The constituents of the fractions were analyzed using high-pressure liquid chromatography (HPLC), and the components were identified by dereplication. Antioxidant potential of the extracts and fractions was investigated using 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging method. Anti-inflammatory and analgesic activities of the extract and fractions were also investigated using xylene-induced inflammation and acetic acid-induced writhing models, respectively. Results: A total of five compounds isovitexin (Rt = 18.77 min), isovitexin-2''-O-xyl (Rt = 19.68 min), p-Hydroxybenzoic acid (Rt = 11.88 min), Sarasinoside L (Rt = 19.64 min), isovitexin (Rt = 18.77), and apigenin monoglycoside (Rt = 19.64 min) were identified by HPLC analysis and dereplication. The ethyl acetate fraction and subfraction elicited the best anti-inflammatory activity. The ethyl acetate subfraction also inhibited acetic acid-induced pain by 79% and 85.0% at the doses of 100 mg/kg and 200 mg/kg, respectively, which was better than 71.1% and 81.3% observed for diclofenac at similar doses. Conclusion: A. nobilis could be a potential source of anti-inflammatory and analgesic lead compounds.
TL;DR: In an MTT assay, embinin showed an inhibition activity higher than the well-known antitumor drug cisplatin against five of the six tested human tumor cells and a significant DPPH radical scavenging activity comparable to the reference antioxidant ascorbic acid.
Abstract: In the first phytochemical investigation of non-volatile secondary metabolites from the Kurdish traditional plant Iris persica L, (-)-embinin was isolated from flowers and leaves, isovitexin from f...
TL;DR: Two new flavonoid glycosides were isolated from the leaves of Moringa oleifera by various chromatographic technologies and their structures were elucidated by spectroscopic methods including UV, IR, MS, and NMR.
Abstract: Two new flavonoid glycosides, quercetin-3-O-(4-O-crotonyl)-β-D-glucopyranoside (1) and quercetin-3-O-[6-O-(2E)-pentenoyl]-β-D-glucopyranoside (2), along with nine known ones, isoquercetin (3), astragalin (4), quercetin-3-O-(6-O-acetyl)-β-D-glucopyranoside (5), kaempferol-3-O-(6-O-acetyl)-β-D-glucopyranoside (6), quercetin-3-O-(6-O-crotonyl)-β-D-glucopyranoside (7), kaempferol-3-O-(6-O-crotonyl)-β-D-glucopyranoside (8), vitexin (9), isovitexin (10), and isorhamnetin-3-O-β-D-glucopyranoside (11), were isolated from the leaves of Moringa oleifera by various chromatographic technologies. Their structures were elucidated by spectroscopic methods including UV, IR, MS, and NMR. In addition, compounds 7 and 8 were isolated from this plant for the first time.
TL;DR: The results of the present study support the development of natural bioactive compounds as cancer chemotherapeutics by revealing that isovitexin gratifies pharmacokinetic bioavailability parameters as evidenced by Lipinski rule and ADMET profile.
01 Apr 2018
TL;DR: The aqueous methanol extract of the aerial parts of C. aegyptiaca afforded seven phenolic compounds for the first time from this species, with promising in-vivo hepatoprotective activity.
Abstract: Objectives: This study aimed to isolate phenolic compounds from the 70% aqueous methanol extract of the aerial parts (leaves, flowers and stems) of Centaurea aegyptiaca , evaluate in-vivo hepatoprotective activity and determine total phenolic and total flavonoid contents. Methods: 70% aqueous methanol extract of the aerial parts subjected to different chromatographic separation techniques. The identities of the isolated compounds were established on the basis of their spectral data and comparing with previously reported data. The crude extract was evaluated for in-vivo hepatoprotective activity on CCl4-induced hepatotoxicity in rats. Total phenolic and total flavonoid contents of the aqueous methanol extract were determined using Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Results: The aqueous methanol extract of the aerial parts of Centaurea aegyptiacaafforded seven compounds. A phenolic acid ester; protochatechuic acid methyl ester (1) along with six known flavonoids; apigenin-6-C-β-D-glucopyranoside (isovitexin) (2), apigenin-8-C-β-D-glucopyranoside (vitexin) (3), quercetin-3-O-b-D-glucopyranoside (isoquercetin) (4), apigenin (5), 3-O-methylquercetin (6) and quercetin (7). Administration of the extract at doses of 100 and 200 mg/kg b.wt showed a hepatoprotective activity similar to that of the standard drug; silymarin at a dose of 25 mg/kg b.wt. Comparative histopathological study of liver exhibited moderate changes in liver histoarchitecture when compared to the CCl4 group. The methanolic extract showed high concentration of phenolic and flavonoid contents. Conclusion: The aqueous methanol extract of the aerial parts of C. aegyptiaca afforded seven phenolic compounds for the first time from this species, with promising in-vivo hepatoprotective activity.
TL;DR: In this paper, the authors used ultra fast liquid chromatography (UFLC) to determine the optimum postharvest handling processes that could retain the phytochemicals quality of C. nutans.
Abstract: Background: Clinacanthus nutans, also known as ‘Sabah snake grass’ or ‘Belalai gajah’, is a herb well known locally for its medicinal values. The primary chemical constituents of the leaves are schaftoside, vitexin, isovitexin, orientin and isoorientin, and antiviral activity is shown by two glycoglycerolipids. Despite the importance of C. nutans, complete information with respect to commercial production and postharvest handling of the herb in the local herbal industry is still lacking. Thus, the objective of this study was to determine the optimum postharvest handling processes that could retain the phytochemicals quality of C. nutans.
Materials and Methods: The flavonoid compounds of C. nutans were analysed by using ultra fast liquid chromatography (UFLC). Total phenolic content and antioxidant activity were determined using a spectrophotometer.
Results: The total phenolic compounds and antioxidant activity in C. nutans were found to be higher in the young vegetative stage than in the mature vegetative stage. Flavonoid compounds (schaftoside, isovitexin, vitexin and orientin) were also found to be highest in the young vegetative plant compared to the mature vegetative plant. All of the assayed phytochemicals and flavonoid compounds levels were found to be highest in oven dried samples compared to the sun, air and solar dried samples.
Conslusion: This study suggests that oven-drying young vegetative C. nutans plant material is the optimum method to retain postharvest quality.
Patent•
17 Apr 2018
TL;DR: In this paper, a microcos paniculata flavone carbon glycoside compound and a preparation method and application of the preparation method are described. But the preparation process of the microcos paniculata is not described.
Abstract: The invention provides a microcos paniculata flavone carbon glycoside compound and a preparation method and application thereof The preparation method of the microcos paniculata flavone carbon glycoside compound comprises the steps that macroporous resin adsorbs a water extract of the microcos paniculata, after elution is conducted sequentially through water and 10% ethyl alcohol, 70% ethyl alcohol elution fraction is collected, the fraction is subjected to acid hydrolysis and neutralization to obtain a transition product; and the transition product is eluted by using 80% methyl alcohol, andthe microcos paniculata flavone carbon glycoside compound is obtained through separation and purification The microcos paniculatat flavone carbon glycoside compound is prepared from the following components of vitexin, isovitexin, apigenin-6,8-C-diglucoside, apigenin-6-C-arabinose-8-C-glucoside, apigenin-6-C-glucose-8-C-arab glycoside, apigenin-6-C-xylose-8-C-glucoside, apigenin-6-C-glucose-8-C-rhamnoside, and apigenin-6-C-rhamnose-8-C-glucoside, wherein the content of apigenin carbon glycoside compounds accounts for 50% or more of the mass of the flavone carbon glycoside compound The microcos paniculata flavone carbon glycoside compound can effectively improve acute lung injury of a model mouse caused by LPS, and has relative good application prospects on development of drugs for treating acute lung injury and lung inflammation
Patent•
23 Feb 2018
TL;DR: In this article, a method for extracting high-purity vitexin and isovitexins from microcos paniculata was proposed, which comprises the following steps of performing alcohol reflux and extracting, filtering, concentrating filtrate, adding ethyl acetate to extract, and concentrating an extracting solution to dryness; adding alcohol into extract to dissolve, adding acid tohydrolyze, adding alkaline to neutralize, separating by macroporous adsorption resin, collecting 60 to 80% of ethyl alcohol eluting solution, and pur
Abstract: The invention relates to a method for extracting high-purity vitexin and isovitexin from microcos paniculata. The method comprises the following steps of performing alcohol reflux and extracting on the microcos paniculata, filtering, concentrating filtrate, adding ethyl acetate to extract, and concentrating an extracting solution to dryness; adding alcohol into extract to dissolve, adding acid tohydrolyze, adding alkaline to neutralize, separating by macroporous adsorption resin, collecting 60 to 80% of ethyl alcohol eluting solution, and purifying by a silicagel column; utilizing a polyamidethin film to perform chromatographic assay, and recrystallizing an eluting solution, so as to obtain pure products of vitexin and isovitexin. The method has the advantages that the effective ingredients in the microcos paniculata can be fully utilized, the pure products of vitexin and isovitexin can be simultaneously obtained, and the added-value of the microcos paniculata is increased; by addingacid to hydrolyze, the extracting rate is increased, and the production cost is favorably reduced.
Patent•
25 Sep 2018
TL;DR: In this article, a Gentiana scabra Bunge flower extraction liquid has been used for whitening and anti-oxidation, which can inhibit melanin secretion of skin melanocytes, has good safety, can be safely used in whitening, antioxidation and antiaging products including health care drugs, foods, beverages, food additives, nutritional supplements or beautifying and skin care products, and has great market potential.
Abstract: The invention relates to a Gentiana scabra Bunge flower extraction liquid, a preparation method and applications thereof. The preparation method comprises: grinding Gentiana scabra Bunge flower into powder, screening, adding water and/or ethanol, extracting at an extracting temperature of 20-80 DEG C, and filtering the extracting liquid to obtain the Gentiana scabra Bunge flower extraction liquid.According to the present invention, the prepared Gentiana scabra Bunge flower extraction liquid contains gentiopicroside, isoorientin and isovitexin, has remarkable effects of whitening and anti-oxidation, can inhibit the melanin secretion of skin melanocytes, cannot stimulate the proliferation of skin cells, has good safety, can be safely used in whitening, anti-oxidation and anti-aging productsincluding health care drugs, foods, beverages, food additives, nutritional supplements or beautifying and skin care products, and has great market potential.
Patent•
28 Jun 2018
TL;DR: In this paper, a process for obtaining bioactive compounds from passion fruit skin is described, which can be used in the food, drug, nutraceutical and cosmetic industries, such as food, drugs, and cosmetics.
Abstract: The present invention describes a process for obtaining bioactive compounds from passion fruit [Passiflora edulis sp) skin. The process is pressurized liquid extraction (PLE) for recovering an extract rich in phenolic compounds such as orientin, isoorientin, vitexin, isovitexin and vicenin. The extract can be used in the food, drug, nutraceutical and cosmetic industries.