Isovitexin

About: Isovitexin is a research topic. Over the lifetime, 526 publications have been published within this topic receiving 11525 citations. The topic is also known as: 6-Glucosylapigenin & Isovitexin.

New apigenin glycoside, polyphenolic constituents,anti-inflammatory and hepatoprotective activities of Gaillardia grandiflora and Gaillardia pulchella aerial parts

Abstract: Background: Gaillardia grandiflora Hort. ex Van Houte and Gaillardia pulchella Foug are flowering plants widely cultivated in Egypt for their ornamental value. Previous reports demonstrated that sesquiterpene derivatives represent the major compounds in both species. Moreover, only few flavones were identified from genus Gaillardia and few studies on the cytotoxicity of G. pulchella were found. Aim of the Study: Investigation of the phenolic constituents of the aerial parts of both species and evaluation of their anti-inflammatory and hepatoprotective activities. Materials and Methods: The 80% aqueous methanol extracts (AME) were prepared for both plants and evaluated for their biological activities. Phytochemical investigation of both extracts resulted in isolation of twelve compounds, which have been identified on the basis of ultraviolet, 1D and 2D nuclear magnetic resonance spectroscopy and negative ESI-MS. Results: The new 8-hydroxyapigenin 6-O-β-D-apiofuranosyl-(1'''→6'')-C-β-D-4C1-glucopyranoside was isolated from G. grandiflora for the first time in nature, along with schaftoside, luteolin 6-C-β-D-4C1-glucopyranoside 8-methyl ether, apigenin 6-C-β-D-4C1-glucopyranoside 8-methyl ether, isoorientin, isovitexin, 6-methoxyluteolin and hispidulin, as well as vicenin-2, vitexin, luteolin and apigenin, which were isolated from G. pulchella together with 6-methoxyluteolin. Furthermore, the AME of both species were found to be nontoxic to mice and exhibited significant anti-inflammatory and hepatoprotective activities in dose dependent manner. Conclusion: Current results shed light on the phenolic constituents of G. grandiflora and G. pulchella aerial parts and the safety of the AME of both species, in addition to their significant anti-inflammatory and hepatoprotective activities. Both plant species may be promising candidates for natural anti-inflammatory and hepatoprotective drugs. Abbreviations used: ALP: Alkaline phosphatase; ALT: Alanine aminotransferase; AME: The 80% aqueous methanol extract of G. grandiflora or G. pulchella aerial parts; AST: Aspartate aminotransferase; br d: Broad doublet; Comp-PC: Comparative paper chromatography; d: Doublet; 2D-PC: Two-dimensional paper chromatography; DMSO-d6: Deuterated dimethyl sulfoxide; G.: Gaillardia; GPx: Glutathione peroxidase; GRd: Glutathione reductase; GSH: glutathione; GST: Glutathione-S-transferase; J: Nuclear spin-spin coupling constant; m: Multiplet; [M-H].: Molecular ion peak; MDA: Malondialdehyde; m/z: Mass/charge ratio; NO: Nitric oxide; p: Probability; PC: Paper chromatography; Rf: Retention flow; rpm: Rotation per minute; s: Singlet; SDE: The ethanol extract of Scoparia dulcis; SE: Standard error; SOD: Superoxide dismutase; TMS: Tetramethylsilane; γmax: Maximum fluorescence emission wavelength.

C-glycosylflavones from Stellaria media

Abstract: AIM: To isolate the chemical constituents from the aerial parts of Stellaria media (L.) Cyr.. METHODS: The compounds were isolated through column chromatography and recrystallization. The structures were elucidated by means of physio-chemical data and spectroscopy. RESULT: Fourteen C-Glycosylflavones were isolated from this plant, and their structures were elucidated as apigenin (1), vicenin (6, 8-di-C-β-D-glucopyranosyl apigenin) (2), 8-C-β-D-galactopyranosyl isovitexin(6-C-β-D-glucopyranosyl-8-C-β-D-galactopyranosyl apigenin) (3), 6-C-β-D-galactopyranosyl vitexin (6-C-β-D-galactopyranosyl-8-C-β-D-glucopyranosyl apigenin) (4), 6, 8-di-C-α-L-arabinopyranosyl apigenin (5), schaftoside (6-C-β-D-glucopyranosyl-8-C-α-L-arabinosyl apigenin) (6), isoschaftoside (6-C-α-L -arabinosyl-8-C-β-D-glucopyranosyl apigenin) (7), 6-C-β-D-galactopyranosyl-8-C-α-L-arabinosyl apigenin (8), 8-C-β-D-galactosyl apigenin (9), vitexin (8-C-β-D-glucopyranosyl apigenin) (10), isovitexin (6-C-β-D-glucopyranosyl apigenin) (11), tricetin 6, 8-di-C-β-D-glucopyranosyl (12), tricetin 6-C-α-L-arabinosyl-8-C-β-D-glucopyranosyl (13) and 7-O-β-D-glucopyranosyl-6-C-β-D-6”-acetyl-glucopyranosyl apigenin (14). CONCLUSIONS: Compounds 3~8 and compounds 12 and 13 have been found in the plant for the first time, and compound 14 is a new C-glycosylflavone.

로스팅 녹두를 이용한 다식 제조 및 품질특성

Abstract: In this study, we manufactured mung bean Dasik (pattern pressed candy) after selecting the optimum roasting conditions through physiochemical analysis and sensory evaluation. Then anti-oxidative abilities of roasted mung bean were measured in order to develop beauty food (Dasik) using roasted mung bean. In the content of vitexin and isovitexin of roasted mung bean, about 10 times the vitexin in the seed(60.85 mg/g) is found in the skin of raw mung bean, and about 9 times the isovitexin in the seed(71.42 mg/g) is also found in the peel. As a result of analyzing the seed and peel of mung bean after roasting it for 10, 20 and 30 minutes respectively, the optimum roasting condition is thought to be 110℃ for 30 minutes as the contents of vitexin and isovitexin showed the highest values of 104.94 mg/g and 122.02 mg/g respectively when the mung bean was roasted at 110℃ for 30 minutes. In the anti-oxidative activity evaluation of the optimum mung bean Dasik, the total content of phenol was shown to be 0.15 mg/mL, and the total content of flavonoid was shown to be 0.026 mg/mL. The DPPH radical scavenging ability showed a high vitality of 58.19%, and the ABTS radical scavenging ability was shown to be 13.26%.

Tyrosinase and Cholinesterase Inhibitory Potential and Flavonoid Characterization of Viola odorata L. (Sweet Violet)

Abstract: Inhibitory potential of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Viola odorata L. (VO) was investigated against tyrosinase (TYR) and cholinesterases by microplate assays. The antioxidant activity was tested using six in vitro assays. Only the ethanol extract inhibited TYR (80.23 ± 0.87% at 100 µg mL-1 ), whereas none of them were able to inhibit cholinesterases. The extracts were more able to scavenge NO radical (31.98 ± 0.53-56.68 ± 1.10%) than other radicals tested, and displayed low to moderate activity in the rest of the assays. HPLC analysis revealed that the aqueous extract of VO contained a substantial amount of vitexin (18.81 ± 0.047 mg g-1 extract), while the ethanol extract also possessed rutin (1.31 ± 0.013 mg g-1 extract) and vitexin (4.65 ± 0.103 mg g-1 extract). Furthermore, three flavonoids (rutin, isovitexin, and kaempferol-6-glucoside) were isolated from the ethanol extract. This is the first report on TYR inhibitory activity of VO as well as presence of vitexin and isovitexin in this species. Copyright © 2015 John Wiley & Sons, Ltd.