Isovitexin

About: Isovitexin is a research topic. Over the lifetime, 526 publications have been published within this topic receiving 11525 citations. The topic is also known as: 6-Glucosylapigenin & Isovitexin.

[Simultaneous determination of four glycosylflavones from Lophatherum gracile by RP-HPLC].

Abstract: Objective To develop a RP-HPLC method for simultaneous determination of orientin, isorientin, vitexin and isovitexin in Lophatherum gracile from different habitat and harvesting time. Method The HPLC method was applied and the chromatographic column was a Waters XBridge C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase consisted of methanol-0.05% acetic acid (35:65). The flow rate was 1.0 mL min(-1) and the detection wavelength was set at 340 nm. The column temperature was set at 25 degrees C. Result Four components were isolated well, the linear relationships were excellent. The mean recoveries and RSD values of orientin, isorientin, vitexin and isovitexin were 103.2%, 2.1%; 101.6%, 2.7%; 98.4%, 2.3%; 99.2%, 1.8%, respectively. Conclusion The HPLC method is simple, sensitive and reliable, and can be used for the quality control of the medicinal material.

Alkaloids and flavonoids exert protective effects against UVB-induced damage in a 3D skin model using human keratinocytes

Abstract: This study isolated six carboline alkaloids, (3S,4R)-1-(β-carbolin-1-yl)-3,4,5-trihydroxy-1-pentanone (1), β-carboline (2), 1-hydroxymethyl-β-carboline (3), perlolyrine (4), (1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (5) and (1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (6) from the leaves of Dendrobium officinale Kimura et Migo (Orchidaceae). These alkaloids, along with 14 flavonoids, vicenin-2 (7), vicenin-1 (8), isoschaftoside (9), schaftoside (10), neoschaftoside (11), violanthin (12), isoviolanthin (13), vicenin-3 (14), apigenin 6-C-α-l-arabinopyranosyl-8-C-β-d-xylopyranoside (15), vitexin 2″-O-glucoside (16), vitexin 2″-O-rhamnoside (17), rutin (18) and quercetin (19), which were previously found in D. officinale leaves by other researchers, and apigenin (20), which is the aglycone of flavone C-glycosides 7–17, were bioassayed to determine their protective effects on UVB-induced damage in human keratinocytes. Carboline alkaloids 1 and 3 and flavonoids 8 and 20 significantly increased cell viability in human keratinocytes irradiated by UVB. In a 3D skin model, compounds 1 and 8 showed promising protective effects, maintained reasonable skin structures and increased the expression of filaggrin and claudin-1, which was inhibited by UVB irradiation. The anti-UVB activities of compounds 1, 3 and 8 were reported for the first time, and these three compounds exhibit potential for application in sunscreen cosmetics.

Pharmacognostical Studies on Gleditsia. III. Flavonoidal Constituents in the Leaves of Gleditsia japonica MIQUEL and G. sinensis LAMARCK

Abstract: Six flavonoids ; luteolin-7-glucoside, isoquercitrin, vitexin, isovitexin, orientin, and homo-orientin were isolated from the leaves of Gleditsia japonica MIQUEL and G. sinensis LAMARCK.