Isovitexin

About: Isovitexin is a research topic. Over the lifetime, 526 publications have been published within this topic receiving 11525 citations. The topic is also known as: 6-Glucosylapigenin & Isovitexin.

Chemical constituents in higher polar substances from Desmodium caudatum

Abstract: In this study the chemical constituents of the higher polar sustances from Desmodium caudatum were investigated.The compounds were isolated by using column chromatographies over silicagel, polyamide, ODS, Sephadex LH-20, and preparative HPLC. The structures of these compounds were identified on the basis of NMR and MS spectra. Thirteen compounds were obtained and their structures were identified as vanillin(1), loliolide(2), indole-3-carboxaldehyde(3), salicylic acid(4), swertisin(5), saccharumoside C(6), isosinensin (7), kaempferol 3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (8), isovitexin (9), vitexin (10), nothofagin(11), resveratroloside (12), and 2"-α-rhamnopyranosyl-7-O-methylvitexin (13). Except for compound 5, the remaining compounds were isolated from D. caudatum for the first time. Compounds 2, 3, 6-8, 11-13 were separated from the genus Desmodium for the first time.

Isolation, Characterization of Bamboo Leaf Flavonoids by Size Exclusion Chromatography and Their Antioxidant Properties

Abstract: The utilization of bamboo industry exhibits varied but still needs to be improved. Bamboo leaf flavonoid (BLF) is an important resource of bamboo which has become a research focus. However, the isolation and purification techniques of four flavonoid carbon glycosides (orientin, isoorientin, vitexin, and isovitexin) from BLF were still confronted with difficulties due to their complex and similar structures, which obstructed the development of bamboo utilization. In this article, a purification technology of four flavonoid carbon glycosides from BLF by Sephadex LH‐20 was improved. The results were evaluated by HPLC and pharmacological activity. Specifically, the eluent, flow rate, and loading amount were investigated, respectively. According to the results, the eluent would dominate the isolation effect among three factors. High concentration of isoorientin and four flavonoid carbon glycosides would be obtained under the optimized condition (The eluent was 70 % methanol, the loading amount was 1.5 g, and the flow rate was 0.5 mL/min). Meanwhile, the link between flavonoid carbon glycosides content and their antioxidant activity in vitro was also revealed. Overall, the results suggested that BLF may serve as potential functional food additives and medicine.

Chemical constituents from Folium Isatidis

Abstract: Objective To isolate and identify the chemical constituents in leaves of Isatis indigotica Fort(Folium Isatidis)Methods The ethanol extract of Folium Isatidis crude drug was extracted with chloroformThe chloroform layer and the aqueous layer were separated by multiple column chromatography including macroporous resin,silica gel and polyamide column and preparative HPLCThe compounds obtained were identified by mass spectra and NMR dataResults Eleven compounds were identified from Folium IsatidisConclusions Among them,isoorientin 3″-O-glucopyranoside is a new compound,and isovitexin 6″-O-glucopyranoside,isoorientin,saponarin,loliolide,ethyl pyrophaeophorbidea,and methyl pyrophaeophorbidea are isolated from Isatis indigotica Fortfor the first time

Determination of Vitexin and Isovitexin in Crotalaria sessiliflora L. by Focused Microwave-assisted Aqueous Two-phase Extraction Coupled with High Performance Liquid Chromatography

Abstract: A new method for analysis of vitexin and isovitexin in Crotalaria sessiliflora L was developed by focused microwave-assisted aqueous two-phase extraction(FMAATPE) coupled with high performance liquid chromatography(HPLC) The experimental factors,including ethanol concentration,ratio of material to solvent,microwave power and extraction time were optimized based on the single factor test and orthogonal test The analysis was performed by HPLC with UV detection at 340 nmThe mobile phase consisted of acetonitrile- water containing 0 5% phosphoric acid(14 ∶ 86) The optimum extraction conditions were as follows: two-phase composition: 35% ethanol-16% ammonium sulfate,ratio of material to solvent: 1 ∶ 50,microwave power: 140 W,extraction time: 20min The method was applied in the determination of vitexin and isovitexin in Crotalaria sessiliflora L,with recoveries of 96 9%-103 8% and RSDs of 1 9%-2 6%