Isovitexin

About: Isovitexin is a research topic. Over the lifetime, 526 publications have been published within this topic receiving 11525 citations. The topic is also known as: 6-Glucosylapigenin & Isovitexin.

Immune regulation oral liquid preparation with anti-cancer efficacy and preparation method of a main component thereof

Abstract: The invention provides an immune regulation oral liquid preparation with anti-cancer efficacy and a preparation method of a main component thereof The main component comprises an extracting solution of fruits such as hawthorn and the like, and the extracting solution is used as a fermentation medium to generate active vitexin and isovitexin with anti-cancer efficacy in the fermentation process through specific yeast; moreover, specific life information is applied to yeast through radio-frequency signals of a specific frequency to excite the yeast to start recessive functional genes for generating vitexin and isovitexin, thereby forming the specific yeast; and then, the specific yeast is added into the extracting solution, and the specific yeast generates more vitexin and isovitexin in the fermentation process under the induction of the vitexin and isovitexin contained in the extracting solution By using the method provided by the invention, a large amount of cheap anti-cancer medicines containing vitexin and isovitexin can be prepared, the medicine expenses of cancered patients can be relieved, and more cancered patients can be effectively treated

Method for quickly measuring content of flavonoid constituents in paniculata

Abstract: The invention discloses a method for quickly measuring the content of flavonoid constituents in paniculata. The method is used for measuring the contents of epicatechin, vitexin, isovitexin and narcissoside in paniculata, wherein the contents can be quickly measured by using an HPLC (High Performance Liquid Chromatography) method. The method can be used for measuring the contents of the four flavonoid constituents, namely the epicatechin, the vitexin, the isovitexin and the narcissosid, in the paniculata simultaneously through one step. The measurement method provided by the invention has the advantages of high speed, simplicity, accuracy, high sensitivity and good stability, and is very beneficial to the application in quick quality control of medicinal materials of the paniculata in a drug preparation process.

Comparative pharmacokinetic studies of five C-glycosylflavones in normal and urolithiasis model rats following administration of total flavonoids from Desmodium styracifolium by liquid chromatography-tandem mass spectrometry.

Abstract: The total flavonoids of Desmodium styracifolium are the flavonoid extracts purified from Desmodii Styracifolii Herba, which has conventionally been used for treating urolithiasis in China. In this study, a sensitive and simple liquid chromatography-tandem mass spectrometry method was developed to simultaneously determine five active components of the extracts in rat plasma. Chromatographic separation of the analytes (schaftoside, vicenin-1, vicenin-2, vicenin-3 and isovitexin) was performed on an ACQUITY UPLC HSS T3 Column under gradient elution conditions. The calibration curves were linear over ranges from 0.5 to 100 ng·mL-1 for schaftoside, vicenin-1, vicenin-2, and vicenin-3, and 0.2 to 20 ng·mL-1 for isovitexin. The RSD of intra- and inter-day precisions were ≤ 6.8% and ≤ 8.3%, respectively, and the accuracies (relative error) were within ±7.6%. The recoveries of the analytes ranged between 97.3 and 100.3%, and the matrix effects ranged from 98.6 to 113.8%. The method was successfully applied to the pharmacokinetic studies of the five active ingredients of Desmodium styracifolium, for the first time, in both normal and urolithiasis model rats. Results revealed that the plasma levels of these components were significantly increased under the pathological state. This study provided valuable information facilitating the clinical investigation of this medicine. This article is protected by copyright. All rights reserved.

Total Phenolic Content and Antioxidant Activity of Flavonoids Isolated From Leaves of Selected Citrus Species

Abstract: Water and 80% methanol crude extracts from the leaves of Citrus aurantifolia, C. hystrix, C. grandis, and Citrofortunella microcarpa were investigated for their total phenolic content and its relationship with total antioxidant activity. The total phenolic content was determined using a Folin-Ciocalteu reagent whereas determination of total antioxidant activity was carried out using a β-carotene bleaching method. Among the citrus leaves extracts, the highest total phenolic content was found from the water extract of Citrofortunella microcarpa whereas the water extract of C. maxima and Citrofortunella microcarpa gave the highest total antioxidant activity and was comparable with the antioxidant activity of marker BHT. However, no clear correlation was found between the total phenolic content and the total antioxidant activity of citrus leaves in both crude extract. Eleven flavonoid components were isolated and identified from the 80% methanolic extract of the leaves of Citrus aurantifolia, C. hystrix, C. grandis and Citrofortunella microcarpa. The isolation and purification of all flavonoid components were carried out using standard paper chromatography technique. The isolated components were identified basis of their spectral analysis with shift reagents, Rf values, colour reactions under UV and UV+NH3, hydrolysis with acid and co-chromatogram with standard markers. The successfully isolated flavonoids were consisted from the group of flavone, flavone C-glycoside and flavonol. The methylated and glycosylated flavonoids were also isolated. The antioxidant capacity of isolated flavonoids was measured by two assays; a β-carotene bleaching method and a DPPH scavenging method. Statistical analysis showed that the total antioxidant and free radical scavenging activity of the isolated flavonoids was significantly low compared to the markers. Among the isolated flavonoids, results showed that at the 60th minute, quercetin 4’-O-glucoside exhibited the highest total antioxidant activity. Quercetin 4’-O-glucoside also showed a significantly higher free radical scavenging activity in DPPH scavenging method than the other isolated flavonoids. The lowest activities in both assays were exhibited by vitexin 7-O-arabinoside and isovitexin.