Topic
Keratan sulfate
About: Keratan sulfate is a research topic. Over the lifetime, 1253 publications have been published within this topic receiving 57984 citations. The topic is also known as: keratan sulfate & KS.
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TL;DR: The high sensitivity of the hydrazine-nitrous acid fragmentation procedure should prove useful in the structural elucidation of cell surface and basement membrane proteoglycans as well as other sulfated glycoconjugates which are present in small amounts.
25 citations
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TL;DR: From the specific 35S radioactivities of the chondroitin sulfate and keratan sulfate preparations, the occurrence of two independent proteoglycan populations is suggested.
Abstract: From human hyaline cartilage (processus xyphoid) preincubated in the presence of [35S]sulfate, proteoglycans were extracted by 4M guanidinium chloride and divided into 6 age groups. Fractionation of proteoglycans by gel filtration under dissociative conditions resulted in two proteoglycan fractions (a and b) with different hydrodynamic volumes. The higher molecular weight fraction a contained chondroitin sulfate, the fraction b keratan sulfate as predominant glycosaminoglycan, the chondroitin sulfate/keratan sulfate ratio decreasing with increasing age in either fraction. The relative portion of proteoglycan fraction b and its 35S-labelling increased with increasing age. From the specific 35S radioactivities of the chondroitin sulfate and keratan sulfate preparations, the occurrence of two independent proteoglycan populations is suggested. A precursor-product relationship between proteoglycan fraction a and b could be excluded.
25 citations
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TL;DR: It is demonstrated that recombinant aggrecan can be used for in vitro analyses of matrix protease-dependent degradation of Aggrecan in the IGD and CS domains, and both recombinantaggrecan and link protein can be use to study the assembly of proteoglycan aggregates with hyaluronan.
25 citations
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TL;DR: Results are thought to indicate the presence of collagen peptides tightly bound to the proteoglycan, which are currently unknown in adult human costal cartilage.
25 citations
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TL;DR: Analysis of KS by means of HPLC showed that in vitro cultured stromal keratocytes express and secrete more KS than keratinocytes, thus mirroring results observed in vivo, and expression of the CHST6 gene and of KS proteoglycans such as mimecan, lumican is limited toStromal Keratocytes.
25 citations