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Keratan sulfate

About: Keratan sulfate is a research topic. Over the lifetime, 1253 publications have been published within this topic receiving 57984 citations. The topic is also known as: keratan sulfate & KS.


Papers
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Journal ArticleDOI
TL;DR: At parturition, a large KS-PG, which is virtually exclusively present in the cervical mucous of either early or nonpregnant women, was detected in the extracellular matrix, indicating that cervical ripening is accompanied not only by quantitative but also by qualitative changes in the composition of the extracllular matrix.
Abstract: To elucidate the function of keratan sulfate proteoglycan (KS-PG) in the human uterine cervix, we analyzed its distribution with respect to physiologic conditions. Immunohistochemistry was used to localize KS bearing proteoglycans (mAb 5D4) and decorin (mAb 6B6) in the lower uterine segment. Proteins present in cervical mucous were labeled with biotin, glycosaminoglycan chains were digested enzymatically, and the samples were analyzed by Western blot. Decorin was detected throughout the extracellular matrix, in tissues from menstruating nonpregnant women, in early pregnancy, from women who had cesarean at term, at postpartum hysterectomy, and from postmenopausal women. In menstruating nonpregnant women, in early pregnancy (first trimester), and in postmenopausal women, KS-PG was detectable only in epithelial, mucous-producing cells. Interestingly, in samples obtained either at the time of cesarean at term (lower uterine segment) or after postpartal hysterectomy, KS-PG was detectable throughout the extracellular matrix, indicating that the expression of KS-PG is associated with reorganization of the tissue. Biochemical analysis of the KS present in mucous revealed a core protein in the range of 220 kDa, suggesting an identity with the large KS-PG described previously. At parturition, a large KS-PG, which is virtually exclusively present in the cervical mucous of either early or nonpregnant women, was detected in the extracellular matrix. This finding indicates that cervical ripening is accompanied not only by quantitative but also by qualitative changes in the composition of the extracellular matrix.

5 citations

Patent
24 Jul 1990
TL;DR: In this paper, a cancer metastasis-suppressing agent containing a glycosaminoglycan bonded with phospholipid or lipid or its salt, having cell adhesion inhibiting action and free from toxicity.
Abstract: PURPOSE: To provide a cancer metastasis-suppressing agent containing a glycosaminoglycan bonded with phospholipid or lipid or its salt, having cell adhesion inhibiting action and free from toxicity. CONSTITUTION: The objective agent contains a glycosaminoglycan bonded with phospholipid or lipid or its salt, e.g. a compound of formula (P 1 is phospholipid having primary amino group; i) the substitution site of GAG is 4 and that of R 3 is 3, R 2 is COOH and R 3 is OH when GAG is a residue produced by removing the reducing terminal glucuronic acid unit from hyaluronic acid, chondroitin, chondroitin sulfate A, C or E, dermatan sulfate, heparin or heparan sulfate or a residue produced by removing the reducing terminal iduronic acid from dermatan sulfate; ii) the substitution site of GAG is 3, that of R 3 is 4, R 2 is CH 2 OH and R 3 is OH when GAG is a residue produced by removing the reducing terminal galactose unit from keratan sulfate; etc.). The dose of the active component for adult is 100-2,000mg/day for oral administration and 10-1,000mg/ dose for injection. COPYRIGHT: (C)1992,JPO&Japio

5 citations

Journal Article
TL;DR: The human intervertebral discs which were obtained by cadavers and anterior discectomy are investigated histochemically and there was no histologically and histochemical observable difference in the cervical and lumbar discs.
Abstract: The human intervertebral discs which were obtained by cadavers and anterior discectomy are investigated histochemically. Chondroitin-4S, chondroitin-6S, dermatan sulfate, hyaluronic acid and keratan sulfate were detected in the human intervertebral disc by various histochemical methods. pH2.5, pH1.1 toluidin blue metachromasia and 0.4M MgCl2 alcianophilia became weaker with increasing age, and the herniated disc were weaker than controlled discs in the same age group. Chondroitin-4S and chondroitin-6S were distributed throughout the discs. There was no clear localization of the various glycosaminoglycans in the human intervertebral disc, with the exception of keratan sulfate. There was no histologically and histochemically observable difference in the cervical and lumbar discs.

5 citations

Journal ArticleDOI
TL;DR: It is found that degenerated cartilage contained an increase in dermatan sulfate and hyaluronic acid and a decrease in chondroitin 6-sulfate and total uronic acid concentration, with only slight changes in chONDroitin 4-solfate and keratan sulfate compared with normal cartilage.
Abstract: We obtained samples of patellar cartilage at the time of surgery from 20 patients: 11 with normal and 9 with macroscopic cartilaginous degeneration. The glycosaminoglycans were separated by cellulose acetateelectrophoresis, and the relative content of major glycosaminoglycans was measured by optic scanning. The total content of glycosaminoglycans was estimated by uronic acid analysis. We found that degenerated cartilage contained an increase in dermatan sulfate and hyaluronic acid and a decrease in chondroitin 6-sulfate and total uronic acid concentration, with only slight changes in chondroitin 4-sulfate and keratan sulfate compared with normal cartilage.

5 citations

Journal ArticleDOI
TL;DR: It is found that a striking deficiency of β‐N‐acetyl‐hexosaminidase activity was present in the skin fibroblasts of patients with Sandhoff disease but not in Tay‐Sachs disease.
Abstract: We have prepared a new substrate from a keratan sulfate-derived-oligosaccharide (2-acetamido-2-deoxyglucosyl-(1--3)-[1-3H] Galactitol), which is necessary to measure beta-N-acetylhexosaminidase activity. This substrate was prepared from a cornea keratan sulfate by digestion with endo-beta-galactosidase, followed by isolation of disaccharide on gel filtration chromatography and chemical desulfation. Using this substrate, we found that a striking deficiency of beta-N-acetylhexosaminidase activity was present in the skin fibroblasts of patients with Sandhoff disease but not in Tay-Sachs disease. Both beta-N-acetyl-hexosaminidase A & H contributed to the catabolism of keratan sulfate.

5 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202310
202222
20217
20209
201912
201812