Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones

Abstract: Morinda elliptica (Rubiaceae) cell suspension cultures were established in shake flask system for the production of anthraquinones. The optimized medium formulation for cell growth and anthraquinone production is proposed. Murashige and Skoog's basal medium (MS) was found to be the best medium, used in combination with 0.5 mg l-1 NAA and 0.5 mg l-1 kinetin. At the range of sucrose concentration tested (3–8% w/v), 8% was the best in enhancing both cell growth and anthraquinone production. A strategy to formulate growth and production medium by manipulating culture age and inoculum age, the type of medium formulation used to grow inoculum, incubation temperature and light intensity was established. By using 18 month old culture and 7 day old inoculum at incubation temperature of 27 ± 3 °C, anthraquinone yield of 2.9 g l-1 and 4.5 g l-1, under illumination of 1200 lux and in the dark was obtained, respectively.

Micropropagation of Salvadora persica-a tree of arid horticulture and forestry

Abstract: A micropropagation method for Jaal (Salvadora persica)—a tree of arid horticulture and forestry has been developed. Nodal segments of fresh shoot sprouts originated from axillary buds obtained from a plant around 35–40 years old lopped plant were used as explants for establishment of in vitro cultures. Surface-sterilized explants produced optimum number of shoots through activation of axillary buds on Murashige and Skoog’s (MS) medium containing 8.88 μM BA (6-benzyladenine) + additives (25 mgl−1 each of adenine sulphate, arginine, citric acid, 50 mgl−1 ascorbic acid). The shoot multiplication was influenced by the successive transfer of the mother explants for 4–5 passages. The maximum number (23.1 ± 0.73 shoots per explant) of shoots were regenerated on MS supplemented with 1.11 μM BA + 1.16 μM Kn (Kinetin) + 0.54 μM NAA (α-naphthalene acetic acid). About 90% shoots pulse-treated with a combination of 2460.27 μM Indole-3-butyric acid (IBA) + 494.56 μM NOA (2-naphthoxy acetic acid) were rooted ex vitro on soilrite within 15–18 days. Over 80% cloned plantlets were hardened successfully in a green house and transferred to polybag/pots.

In vitro flowering of bitter melon.

Abstract: Flowers were formed from shoot tips of bitter melon (Momordica charantia L.) cultured on Murashige and Skoog medium supplemented with 90 mM sucrose, 0.05 mM Fe2+ and 4 µM N6-benzyladenine (BA). The addition of 0.05 mM Fe2+ to the medium prevented chlorosis of the explant and promoted normal flowering. Increasing the ratio of carbon to nitrogen promoted male flower formation but intensively inhibited vegetative growth. The influence of cytokinin on the morphogenesis of the explant was highly notable. Flowers could be formed after a 15- to 20-day exposure to kinetin (Kin) or BA. Kin and BA had opposite effects with regard to the development of the explant. Kin promoted flower formation, especially female, but inhibited branch bud formation. Conversely, BA promoted branch bud formation and also promoted male flower formation when present at a concentration of 1–2 µM, but completely inhibited flower formation at 4–8 µM. Fluorescein diacetate staining and in vitro germination showed that in vitro pollen were of a fairly high viability.

In vitro multiplication of Ocimum gratissimum L. through direct regeneration

Abstract: The objective of this study was to develop a rapid system for regeneration of the important medicinal plant, Ocimum gratissimum L, from nodal explant. Single node explants were inoculated on basal MS (Murashige and Skoog, 1962) medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) for direct plant regeneration. Maximum numbers of shoot (14.3± ± ± ±1.5) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on same half strength MS medium supplemented with 0.5 mg/l of IAA alone for three weeks. Well-developed complete plantlets were transferred on to specially made plastic cup containing soilrite. Acclimatized plantlets were successfully grown in garden soil.

Regeneration of pea (Pisum sativum L. cv. Century) plants by in vitro culture of immature leaflets.

Abstract: In vitro regeneration of plants from immature leaflets of 3 day-old pea (Pisum sativum L. cv. Century) seedlings was studied under defined nutritional, hormonal and environmental conditions. Immature leaflets isolated from the second and third apical leaves of aseptically germinated seeds were cultured on MS medium containing vitamins as in B5 medium, 3% sucrose, 0.8% agar and supplemented with 0.1, 1, and 10 μM concentrations of naphthaleneacetic acid (NAA) and 1 and 10 μM levels of benzyladenine (BA) in various combinations. Shoot regeneration from the primary callus occurred within 45 to 90 days of culture in most of the hormone combinations. Although the number of calli producing shoots was maximal at 10 μM levels of NAA and BA, multiple shoot regeneration was predominant at a combination of 0.1 μM NAA and 10 μM BA. Indoleacetic acid (IAA) and kinetin (K), both at 10 μM, also induced shoot regeneration. No shoots were regenerated when 10 day-old leaflets were used as explants. Root production generally occurred on non-shoot regenerating calli. Roots were induced to differentiate by transferring the regenerated shoots onto half-strength B5 medium supplemented with 1 μM NAA.