Topic
Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: By culturing portions of lentil shoot meristems and epicotyls on a medium containing kinetin and gibberellic acid, callus tissue was produced which could be induced to regenerate shoots in relatively large numbers, even after several subcultures.
Abstract: Until recently, grain legumes in general have proven recalcitrant at de novo regeneration in vitro. By culturing portions of lentil (Lens culinaris) shoot meristems and epicotyls on a medium containing kinetin and gibberellic acid, callus tissue was produced which could be induced to regenerate shoots in relatively large numbers, even after several subcultures. The shoots could be rooted in a mist chamber to yield whole, fertile plants.
52 citations
TL;DR: The rooted plantlets were acclimated to ex vitro conditions and a survival percentage > 70% was obtained under greenhouse conditions and this work was carried out as an ex situ conservation method for these Spanish endemic species.
Abstract: Salvia valentina Vahl and Salvia blancoana Webb & Heldr subsp. mariolensis Figuerola, two endemic species of Salvia from the Mediterranean coastal region of Spain, were successfully gegenerated in vitro from adult plants using two explant types (apical and nodal segments). Maximum shoot proliferation for both species was obtained with nodal explants: for S. blancoana on Murashige and Skoog medium supplemented with 6-γ-γ-dimethylallylaminopurine at 1 mg 1−1 (4.9 μM). and for S. valentina on the same medium with kinetin at 1–2 mg 1−1 (4.6–9.3 μM). The influence of apical dominance, and the explant viability in culture were found to be the main differences between the two species during the shoot multiplication phase. Rooting of shoots was low, specially for S. valentina. For both species, rooting was achieved in Murashige and Skoog medium without growth regulators. In general the addition of the auxins indole 3 acetic acid or indole-3-butyric acid did not improve the rooting or, in the case of naphthaleneacetic acid, had an inhibitory effect. In the best rooting media, rooting shoots increased in length. The rooted plantlets were acclimated to ex vitro conditions and a survival percentage > 70% was obtained under greenhouse conditions. This work was carried out as an ex situ conservation method for these Spanish endemic species.
52 citations
TL;DR: A cytological analysis revealed the karyotypical heterogeneity of the garlic callus cells, and 2,4-D appeared to be a strong dedifferential factor blocking the mechanism of organogenesis but not histogenesis of the protracheal elements in garliccallus cultures.
52 citations
TL;DR: The development and optimization of an effective micropropagation protocol can give an important contribution to improve the quality of pistachio plants and, as a consequence, of orchard production in Middle East countries.
52 citations
TL;DR: Regenerated plantlets could be sucessfully transferred to soil where they grew well within 10–12 weeks with 80% survivality and shoot proliferation could be continued even after a year by transferring each divided shoot explant to the same medium.
Abstract: Emerging buds of rhizome of Alpinia galanga Willd produced shoots and roots simultaneously when cultured in MS medium supplemented with kinetin 3.0 mg l-1. Each explanted shoot bud produced 8 shoots in average and roots simultaneously within 8 weeks. Shoot proliferation could be continued even after a year by transferring each divided shoot explant to the same medium. Regenerated plantlets could be sucessfully transferred to soil where they grew well within 10–12 weeks with 80% survivality.
52 citations