Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

The mechanism of kinetin-induced transport in corn leaves.

Abstract: 1. Kinetin-induced transport of P32 in detached corn leaves is shown to be limited to the axial direction of the leaves, i.e. along the axis of the vascular bundles. It is not apparently dependent on the water flow in the xylem, and it can be blocked by steam-killed zones or by metabolic inhibitors. It is concluded that kinetin-induced redistribution of phosphates in isolated corn leaves takes place in the phloem. 2. The movement of P32 is preferentially toward the base of the excised leaf, indicating a natural mobilization center at the leaf base. 3. Kinetin treated parts of leaves attract and accumulate P32. They do not accumulate the radioisotopes Na22, Rb86, Cl36 and I131; but the transport of Na22 directed towards kinetin centers is enhanced. 4. Two “mobilizing centers” established by kinetin application along the leaf axis do not attract P32 from intermediate tissue at the same time in opposite directions. They compete with each other for the transport system: both the direction and the velocity of movement are determined by the difference of the “mobilizing forces”. Thus, mobilization is either to one or to the other mobilizing center. 5. Under conditions of enhanced transport (i.e. in presence of kinetin centers), the distribution curves of P32 and Na22 are flattened. This change would be expected for the mass-flow type of transport. 6. It is concluded that “mobilizing centers” in isolated corn leaves stimulate a mass-flow transport in the phloem. Evidence is presented that they act as suction pumps. Some general aspects of phloem transport are discussed.

Influence of plant growth regulators, polyamines and glycerol interaction on growth and morphogenesis of carposporelings of Grateloupia cultured in vitro

Abstract: The influence of the plant growth regulators 2,4-D, GA3, BA and kinetin, and the polyamines putrescine, spermidine and spermine were tested on axenic in vitro cultures of carposporelings of Grateloupia doryphora. The auxin 2,4-D (10-3 M) and the polyamine spermine (10-6 M and 10-3 M) induced a callus (disorganised cell mass that arose from the organised tissue of the carposporeling, as demonstrated by microscopic monitoring of the tissue). Putrescine and spermidine (10-3 M) transformed the carposporelings into cell masses that produced shoots. BA (10-3 M) and kinetin (10-6 M and 10-3 M) were inhibitory. In 10-1 M glycerol-containing culture medium, which is known to induce the formation of morphogenic cell masses, the addition of GA3 M) resulted in the inhibition of the morphogenesis (i.e. shoot emission) in the cell mass. The kinetin at 10-6 M inhibited morphogenesis, whilst at 10-3 M inhibited even the formation of the cell masses. The combination of glycerol (10-1 M) and the auxin 2,4-D (10-6 and 10-3 M) or the polyamines putrescine, spermidine and spermine (10-6 and 10-3 M) resulted in a bigger size of the cell masses that led to a higher amount of shoots per cell mass than in glycerol alone.

Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

Abstract: Bambusa nutans Wall., is an evergreen, perennial, and multipurpose bamboo having strong culms, which are largely used for construction, scaffolding, craft purposes, pulp, and paper industry. Multiple shoots from nodal segments (3–4 cm) of young branches of mature culms were established in Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) (1.0–6.0 mg l−1) or in combination with α-naphthaleneacetic acid (NAA) (0.5–1.0 mg l−1) or kinetin (Kn) (1.0–2.0 mg l−1). February–March and December were found to be the best seasons for culture establishments. Maximum shoots were achieved on MS medium fortified with BAP (2.0 mg l−1). Embryogenic callus (slightly greenish compact, globular, and slow growing) was initiated from the base of severed sprouted buds in 2–3 subsequent subcultures on MS medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (5.0 mg l−1) under dark incubations. Maturation and germination of well-organized somatic embryos was achieved on MS medium containing BAP and 2,4-D (1.0 mg l−1 each) with 20.0 mg l−1 ascorbic acid. Full-strength MS medium supplemented with 2% glucose favored further development of proliferated somatic embryos into plantlets. Genetic variations of field-established B. nutans plants regenerated through tissue cultures were assessed by amplified fragment length polymorphism (AFLP) analysis using 6 primer combinations. Four hundred and seven scorable fragments were amplified, of which 402 (98.8%) have recorded conservation at various morphogenetic stages leading to plantlets regeneration, therefore, revealed a high level of genetic stability.

An efficient protocol for in vitro propagation of carnation (dianthus caryophyllus)

Abstract: The present research work involves shoot formation, their multiplication and rooting in carnation Dianthus caryophyllous. For shoot formation both apical and nodal meristems were used. MS medium containing BAP alone or in combination with kinetin was tested. Best shoot formation response was obtained after 6 days of inoculation from apical meristem and after 7 days of inoculation from nodal meristem on MS medium supplemented with 4.0 mg/l BAP. Apical meristem showed more pronounced effect for shoot formation than nodal meristem. Well-developed shoots were shifted for their multiplication. Maximum number of multiple shoots were obtained on MS medium containing 1.0 mg/l BAP. These multiple shoots increased in their number when were given subsequent incubation period. Addition of Kinetin to BAP failed to show good shoot multiplication response. Shoots after attaining the size of 5.0 cm were shifted for rooting. Best rooting response was obtained on MS medium containing 1.0 mg/l NAA. Well rooted plants were shifted into glass house for hardening and acclimatization and were shifted to natural climatic conditions.