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Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.


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Journal ArticleDOI
TL;DR: Some of the histological changes which occur in excised, etiolated sections of the Pisum epicotyl after treatment with 2,4-dichlorophenoxyacetic acid or indoleacetic Acid, with and without kinetin, and also after growing decapitated plants on intact roots in 2, 4-D solutions are described.
Abstract: growth of this callus causes a characteristic split at the base of the internode. Treatment with kinetin, alone or in combination with the auxin, changes the above structure markedly. It leads to the initiation, over the entire circumference of the core of the internode, of a still more active cambium, which forms several layers of secondary xylem; this consists mainly of long vessel members with pitted walls. Hyperplastic growth is completely absent, and the xylem does not become occluded. Thus the effect of kinetin is to make the xylem more normal and to alter the epicotyl structure from herbaceous to more-or-less woody. THE INITIAL purpose of this investigation was to study the effects of auxins and kinetin on the development of the transport system in the stem and bud of the pea plant during the inhibition of lateral bud development. As the work developed, however, it became clear that the changes in the internode are much more extensive than had been expected, and that they required detailed study before changes in the other parts could be interpreted. This paper, therefore, describes some of the histological changes which occur in excised, etiolated sections of the Pisum epicotyl after treatment with 2,4-dichlorophenoxyacetic acid or indoleacetic acid,2 with and without kinetin, and also after growing decapitated plants on intact roots in 2, 4-D solutions. Attention is devoted here to the internode, and a description of the changes in the node and bud will be given in another paper. There are many earlier studies of the anatomy of stems in relation to treatment with auxins,

50 citations

Journal ArticleDOI
TL;DR: Somatic tissue cultures of Paspalum scrobiculatum, Eleusine coracana and Pennisetum typhoideum established on MS medium containing CM and 2,4-D developed shoot buds on abrupt withdrawal of 2, 4-D from the medium or its substitution by low concentrations of NAA or IA A.

50 citations

Journal ArticleDOI
TL;DR: The effects of 60 Co gamma radiation on seeds, seedlings and callus tissue cultures of Phaseolus vulgaris L. var.

50 citations

Journal ArticleDOI
TL;DR: Well developed regenerated plantlets were hardened, acclimatized and established in field, where they grew well without any detectable variation, and a 3 % Na2-alginate with 100 mM CaCl2 has been found to be optimum concentration for the production of uniform synthetic seed.
Abstract: An efficient protocol for encapsulation of nodal segments of Vitex negundo L. has been developed for the production of non-embryogenic synthetic seeds. The encapsulations of nodal segments were significantly affected by the concentrations of sodium alginate and calcium chloride. A 3 % Na2-alginate with 100 mM CaCl2 has been found to be optimum concentration for the production of uniform synthetic seed. For germination, the synseeds were cultured on Murashige and Skoog (MS) basal medium supplemented with kinetin (KIN) and α-naphthalene acetic acid (NAA) either singly or in various combinations. MS medium containing 2.5 μM KIN in combination with 1.0 μM NAA was found to be the optimum for maximum (92.6 ± 3.71 %) plantlet conversion frequency. Well developed regenerated plantlets were hardened, acclimatized and established in field, where they grew well without any detectable variation.

50 citations

Journal ArticleDOI
TL;DR: A simple and efficient micropropagation method was established for direct protocorm-like body (PLB) formation and plant regeneration from flower stalk internodes of a sympodial orchid, Epidendrum radicans, and the regenerated plants grew well with an almost 100% survival rate when acclimatized in a greenhouse.
Abstract: A simple and efficient micropropagation method was established for direct protocorm-like body (PLB) formation and plant regeneration from flower stalk internodes of a sympodial orchid, Epidendrum radicans. Small transparent tissues formed on surfaces and cut ends of flower stalk internodes on a modified half-strength Murashige and Skoog basal medium with or without thidiazuron (TDZ) after 1–2 wk of culture. In the light, the transparent tissues enlarged and turned into organized calluses on most of the explants. However, PLBs formed only on a medium supplemened with 0.45 μM TDZ within 2 mo. of culture. Sucrose, NH4NO3, and KNO3 were used in media to test their effects on PLB proliferation and shooting. The best response on number of PLBs per tube was 23.6 at 40 gl−1 sucrose, 825 mgl−1 NH4NO3, and 950 mgl−1 KNO3, and the highest number of PLBs with shoots was found at 10 gl−1 sucrose, 825 mgl−1 NH4NO3, and 950 mgl−1 KNO3. Homogenized PLB tissues produced by blending were used to test the effects of four cytokinins [TDZ, N6-benzyladenine (BA), zeatin-riboside, and kinetin] on PLB proliferation and shoot formation. The best responses on number of PLBs per tube, proliferation rate, and number of PLBs with shoots per tube were obtained at 4.44 μM BA, 0.28 μM zeatin-riboside, and 1.39 μM kinetin, respectively. Normal plantlets converted from PLBs on the same TDZ-containing medium after 1 mo. of culture. The optimized procedure required about 12–13 wk from the initiation of PLBs to plantlet formation. The regenerated plants grew well with an almost 100% survival rate when acclimatized in a greenhouse.

50 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023115
2022243
2021139
2020137
2019156
2018189