Topic
Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation and the highest concentration of proline, 5.8 µmol g−1(f.m.), was obtained on 164.7 mM sor Bitol combined with 116.8 mM sucrose.
Abstract: This study investigated the influence of osmotic stress, induced by sorbitol and sucrose combinations, on growth and proline accumulation in callus cultures of rice (Oryza sativa L.). Dehusked mature seeds, cv. Hassawi, were induced to callus on MS medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM 6-furfurylaminopurine (kinetin). The medium also contained 29.2, 58.4, 87.6, and 116.8 mM sucrose combined with 0, 54.9, 109.8, and 164.7 mM sorbitol. Callus formation was observed in about 35 % of the cultured seeds irrespective of the sugar treatment. An increase in callus mass was observed as sucrose concentration increased reaching a maximum growth at 87.6 mM. Callus growth was enhanced in response to 54.9 mM sorbitol but at higher concentration it was inhibitory. Best callus growth was obtained on a medium containing 54.9 mM sorbitol combined with 87.6 mM sucrose. Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation and the highest concentration of proline, 5.8 µmol g−1(f.m.), was obtained on 164.7 mM sorbitol combined with 116.8 mM sucrose.
50 citations
TL;DR: It is suggested that endogenous cytokinins are primary factors in the initiation of radicle growth, while gibberellins are important in cotyledon expansion and abscisic acid appears to have an inhibitory role in both processes, and the interactions of these regulators in the control of germination and development are discussed.
Abstract: Dormancy of intact sycamore (Acer pseudoplatanus) seeds was broken by chilling (5°C) for several weeks in moist conditions. Treatment of unchilled seeds with kinetin induced some germination, but gibberellin was ineffective. This stimulation by kinetin was not suppressed by the added presence of abscisic acid during incubation.
50 citations
TL;DR: Preliminary data indicate that most of the culture-induced variant phenotypes were similar to known spontaneous mutation in sunflower and may have single-gene recessive inheritance.
Abstract: Adventitious buds were induced from in vitro culture of sunflower (Helianthus annuus L) cotyledons Four inbred lines (G1, G2, G3 and HA89), an open pollinated variety (‘Argentario’) and two hybrids with specific genetic markers were used Cotyledons were cultured in vitro on MS medium (Murashlge and Skoog 1962) containing various concentrations of kinetin and indole 3-acetic acid (IAA) The quantitative interactions between auxin and cytokinin, the age of the cotyledons and the 2,3,5-triiodobenzoic acid (TIBA) treatments have been found to influence shoot regeneration The plantlets, after rooting, were successfully established on soil Qualitative variation was noted in self-pollinated progeny of plants regenerated from culture of two inbreds Chimerism in regenerated plants was indicated by sectoring of the genetic markers Some culture-induced variant phenotypes were similar to known spontaneous mutation in sunflower but others have been not yet described Preliminary data indicate that most of them may have single-gene recessive inheritance
50 citations
TL;DR: A protocol for Coffea arabica L. cvs.
50 citations
TL;DR: The morphogenic pathway of adventitious shoot regeneration at the ends of Vigna mungo epicotyl segments was determined by ontogenic age, orientation, polarity and position of the explant on mother seedling as well as when the orientation of explants deviates from the normal vertical upright position.
50 citations