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Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.


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Journal ArticleDOI
TL;DR: The kinetics of growth and RA production suggested that the increase in final RA content was due to both an increase in the rate of RA synthesis and initiation of the period of synthesis in the exponential rather than the linear growth phase.
Abstract: Cell suspension cultures of Anchusa officinalis required exogenous phytohormones for their normal growth. Cell lysis was observed at the third passage in a hormone-free medium. Using hormone — depleted cells, the effects of auxins (2,4-D, NAA, IAA and CFP) and cytokinins (BA, kinetin, and zeatin) on cell growth and RA production were investigated. All auxins tested could maintain growth and integrity of the cells whereas cytokinins alone could not, suggesting that this culture is auxindependent. Among the auxins tested, NAA had a pronounced effect on RA production. The total RA content obtained at optimum NAA concentration (0.25 mg/l) reached 1.7 g/l (12% of dry weight). The kinetics of growth and RA production suggested that the increase in final RA content was due to both an increase in the rate of RA synthesis and initiation of the period of synthesis in the exponential rather than the linear growth phase.

42 citations

Journal ArticleDOI
TL;DR: The chlorophyllous cultures grew slowly in a medium with low(0,5%) sucrose or without any carbohydrate source, suggesting photomixotrophism, and release of iignin into the nutrient medium was observed in several experiments, especially in slow-growing cultures supplemented with sucrose.
Abstract: Liisa Kaarlira Simola, Juha Lemmetyinen and Arja SantiineiiSimola, L, K., Lemmetyinen, J, and Santanen, A, 1992. Lignin release and photo-mixotrophism in suspension cultures of Picea abies, - Physiol. Plant. 84: 374-379,The effect of different concentrations of sucrose (0-4%) and of two growth regulators(0-50 |XiW 2,4-D and 0-25 \iM kinetin) was tested on growth and chlorophyll contentof suspension cultures of Picea abies (L,) Karst, originating from chiorophyllousembryo callus in an elevated CO2 (2%) atmosphere, A contiottous chlorophylloussuspension culture was achieved on a meditim containing 2% sucrose and a low levelof organic nitrogen (0,25 mM arginine and 0.5 mM glutamine) suppiemeoted with2,4-D (0,5 iiM) and kinetin (2,5 IiM). The same medium with 4% sucrose gave thebest growth response, but a negative correlation between chlorophyll level andgrowth was observed. The chlorophyllous cultures grew slowly in a medium with low(0,5%) sucrose or without any carbohydrate source, suggesting photomixotrophism,A high coneentration of kinetin inhibited both growth and chlorophyll synthesis.Release of iignin into the nutrient medium was observed in several experiments,especially in slow-growing cultures supplemented with sucrose. Only a few successivepassages of suspensions that produced lignin could be cultured before cell death. Thecultures releasing lignin may be tiniqtie for studies on synthesis and biodegradation ofthis very complex compound.Key words - Chlorophyll, growth, lignin release, photomixotrophy. Picea abies,suspension culture.L, K. Simola (corresponding author) and A, Santanen, Dept of Botany, Vniv, ofHelsinki, Unionink. 44, SF-00170 Helsinki, Finland; J. Lemnwtyinen (presetit ad.-dress), Dept of Biology, Univ, of Joensuu, PL Ul, SF-80101 Joensuu, Finland,

42 citations

Journal ArticleDOI
TL;DR: Tissue culture techniques were applied to study the regeneration and growth of bulblets from bulb scale segments of Crinum macowanii Bak.
Abstract: Tissue culture techniques were applied to study the regeneration and growth of bulblets from bulb scale segments ofCrinum macowanii Bak. (bush- or march lily)in vitro. Shoots were induced on twin scales taken from the basal plate region of flowering-size bulbs on Murashige and Skoog (MS)-medium containing 0–20 mg l−1 NAA and BA and a modified MS medium (MMS medium) containing 1.25 mg l−1 ancymidol (A-RestTM), 0.1 mg l−1 NAA and 0.1 mg l−1 kinetin (ANK). Large bulblets could only be initiated on the latter. Subsequently the bulblets of 5 mm or more in diameter were trimmed and split in half, and secondary plantlets were regenerated on MMS-medium containing ANK or MS-medium without any growth regulators which in turn grew into bulblets suitable for splitting within 12–16 weeks. A total of 700–1000 bulblets could be obtained from each initial bulb within 12 months. Anatomical studies showed that the shoots were initiated from the epidermis and hypodermis on the abaxial surface of the meristematic tissue of the basal plate of the bulb scale. This technique is useful for the multiplication and preservation of a genotype, since plantlets regenerated in this manner should be genetically uniform.

42 citations

Journal ArticleDOI
TL;DR: No significant changes in the composition of chromatin were found when the excised barley leaves were floated on water or on kinetin solution in the dark, but increases in the activities ofchromatin associated ribonuclease and deoxyribonucleasing in leaves floated onWater drastically suppressed the increases in these enzymes.

42 citations

Journal ArticleDOI
TL;DR: In the course of their development the protonemata of Funaria hygrometrica produce two different substances which diffuse into the substrate, which influence bud formation at an optimum only when there is a certain balance between these two substances.

42 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023115
2022243
2021139
2020137
2019156
2018189