Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

Kinetin and Gibberellic acid (GA3) act synergistically to produce high value polyunsaturated fatty acids in Nannochloropsis oceanica CASA CC201

Abstract: Two functionally different plant growth regulators, Kinetin and GA3, were evaluated individually or in combination for its effect on growth, lipid yield, PUFAs and EPA accumulation in Nannochloropsis oceanica CASA CC201 . It was observed that the treatment with 0.215 ppm Kinetin resulted in high cell number of 521 × 10 6 cells per mL than the control (398 × 10 6 cells per mL), but GA3 had an adverse effect on cell number. Kinetin increases the specific growth rate to 0.24/day and doubling time to 2.86 days than control (4.38 days). Treatment with GA3 at a concentration of 50 ppm gives the highest cellular lipid accumulation of 61.5% DCW than the control (35.5% DCW). The combination of Kinetin and GA3 exhibited a synergistic effect on total lipid yield of 246.25 mg/L compared with control (121.5 mg/L). The addition of Kinetin increases the percentage of EPA 4 times as compared to the control.

Comparative performance of in vitro multiplication in four grape (Vitis spp.) rootstock genotypes.

Abstract: The magnitude of demand for planting materials in grape, mainly for rootstock genotypes indicates that micropropagation is inevitably necessary for their mass scale propagation. Therefore, the studies on micropropagation of four genetically different grape rootstocks namely Dogridge (Vitis champini), SO4 (V. riparia× V. berlandieri), H-144 (V. vinifera × V. labrusca) and 3309 C (V. riparia × V. rupestris) were conducted to develop an optimized protocol and to compare in vitro behavior of these genotypes. Culture establishment using nodal segements was enhanced using different growth regulators. Though culture establishment increased using either BAP (Benzyl amino purine) or KIN (Kinetin) but the treatment, 2.0 mgl -1 BAP + 0.2 mgl -1 NAA (Naphthalene acetic acid) was most effective with regard to enhancement in culture establishment and reduction in time to bud sprouting. Least success (38.31%) in culture establishment was observed for H-144 but it exhibited better vegetative growth and rooting among genotypes, i.e. higher shoot multiplication rate (12 microcuttings per culture), highest rooting (87.7%) and early root initiation (11.52 days). Addition of activated charcoal to the rooting medium was found beneficial with respect to enhancement of rooting and minimizing time to root initiation in different genotypes. Among the rootstock genotypes, 3309 C was found most responsive in terms of higher ex vitro plantlet survival (84.95%) during hardening and shorter duration required for ex vitro transfer. These results indicate that multiplication of these grape rootstocks can be performed efficiently by means of direct shoot proliferation using nodal segments from field grown vines. The influence of different factors like culture medium and genotype on the overall micropropagation of grape rootstocks is discussed.

Plant growth regulators affect biosynthesis and accumulation profile of isoflavone phytoestrogens in high-productive in vitro cultures of Genista tinctoria

Abstract: The influence of plant growth regulators on biomass growth and the accumulation of medicinally-relevant isoflavone phytoestrogens, derivatives of genistein and daidzein (8 compounds including aglycones, glucosides and glucoside esters) in callus cultures of Genista tinctoria (Fabaceae) was examined. The experiments included 10 auxins [2,4-dichlorophenoxyacetic acid (2,4-D), p-chlorophenoxyacetic acid, indole-3-acetic acid, indole-3-butyric acid, indole-3-propionic acid, 1-naphthaleneacetic acid, β-naphthoxyacetic acid, picloram, 2,3,5-triiodobenzoic acid (TIBA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T)] and 7 cytokinins [6-benzylaminopurine, forchlorfenuron, 1,3-diphenylurea, 2-isopentenyladenine, kinetin (KIN), thidiazuron, zeatin] applied at 0.5 and 5.0 mg l−1, jointly with 5.0 or 0.5 mg l−1 KIN or 2,4-D (for auxins and cytokinins, respectively—36 phytohormone combinations in total). Statistical analysis of the relationships between callus growth [expressed as growth index (Gi)] and the accumulation of isoflavones showed positive correlation in the cytokinin group (rxy values from 0.13 to 0.61) and negative correlation within auxins (rxy values from −0.31 to −0.39). Among the cytokinins tested, the highest isoflavone content (6,436.26 mg/100 g dry weight) and the fastest biomass growth (Gi = 892.46 %) were obtained for 0.5 mg l−1 KIN used jointly with 5.0 mg l−1 2,4-D. In the group of auxins, the combination of 0.5 mg l−1 TIBA and 5.0 mg l−1 KIN provided the fastest culture growth (Gi = 983.07 %) and the isoflavone concentration of 10,474.23 mg/100 g dry weight, which is so far the highest amount of these metabolites achieved in callus cultures of higher plants.

Efficient micropropagation of Ensete ventricosum applying meristem wounding: a three-step protocol

Abstract: A highly efficient three-step protocol for in vitro propagation of Ensete ventricosum (enset) was developed that consisted of initiation, bud proliferation, and shoot elongation and rooting stages. At the initiation stage, it was crucial to use shoot tips (5–8 mm) with subtending corm tissues as explants to obtain growth. The addition of 0.5–1% (w/v) activated charcoal to the medium was essential to prevent phenol exudation which otherwise leads to the loss of cultures. During the bud proliferation stage, modified MS macronutrients and micronutrients together with a combination of cytokinins (1.6 μM naphthaleneacetic acid, 4.4 μM 6-benzylaminopurine, 23.2 μM kinetin, 22.6 μM N6 2-isopentyladenine) was used. This novel composition of macronutrients was based on the analysis of leaf nutrient content of glasshouse-grown enset sprouts. Multiple bud formation on the enlarged corm tissue was induced only when the meristem region was wounded before transfer to the bud proliferation medium. Up to 75 healthy shoots per explant were produced, whereas unwounded explants produced, only one to two shoots per explant. A third stage with a low concentration of cytokinin enabled shoot elongation as well as root development. The plantlets were acclimatized with 100% success and they showed no apparent phenotypical deviation.

Plantlet formation from callus and shoot-tip culture of Helianthus annuus (L.)

Abstract: A method for callus induction, adventitious bud regeneration, shoot multiplication and rooting of in vitro formed shoots of Helianthus annuus L. var. Argentario is described. Hypocotyl and cotyledon explants formed callus on medium containing 2 mgl−1 naphthalene acetic acid and 0.5 mgl−1 benzyladenine. Adventitious buds were formed on hypocotyl segments on medium containing 0.5–2 mgl−1 benzyladenine. The optimal level of sucrose concentration for shoot regeneration from hypocotyls was 1.5%. Multiplication from shoot apices was promoted by kinetin (2 mgl−1) plus gibberellic acid (5 mgl−1), benzyladenine (2 mgl−1) plus gibberellic acid (10 mgl−1) or at lower frequency by benzyladenine (1 mgl−1). A general feature of the plantlets formed in vitro was the “precocious flowering”.