Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

Use of plant growth regulators in micropropagation of Kappaphycus alvarezii (Doty) in airlift bioreactors

Abstract: The effects of plant growth regulators on cal- lus induction rate and regeneration of K. alvarezii ex- plants was evaluated. K. alvarezii calluses were induced in vitro with kinetin (K), 6-benzylaminopurine (B), 1- naphtalene acetic acid (N) and spermine (S). After 30 days, K. alvarezii explants produced filamentous cal- luses and isolated crystalline filaments growing from the medullar region and from cortical cells at the cut edge. The plant growth regulators 1-naphtalene acetic acid (1 mg L −1 ) and 6-benzylaminopurine (1 mg L −1 ) and the 1-naphtalene acetic acid + kinetin + spermine (1, 1, 0.018 mg L −1 respectively) combination pro- duced 85 to 129% more calluses, with significant differ- ences versus the control ( p < 0.05). Spermine at 0.018 mg L −1 produced calluses in the apical, intercalary and basal regions of explants. Spermine also reduced callus induction time to 7 days, which is faster than previ- ously reported induction times with other plant growth regulators. An airlift bioreactor was designed and char- acterized to micropropagate K. alvarezii calluses. The bioreactor had mixing times ranging from 4.6-10.3 sa tT90 and T95, which is shorter than those for the

Effect of Kinetin on Powdery Mildew

Abstract: IN work on wheat stem rust, Person et al.1 observed that detached leaves from resistant plants which become susceptible and chlorotic when floated on water maintain their green colour and rust resistance wiien floated on a kinetin solution. A review of the effects of kinetin (6-furfurylaminopurine) on various plant tissues has been made by Parthier2.

Introduction of OsglyII gene into Oryza sativa for increasing salinity tolerance

Abstract: Mature seed-derived embryogenic calli of indica rice (Oryza sativa L. cv. PAU201) were induced on semisolid Murashige and Skoog medium supplemented with 2.5 mg dm -3 2,4-dichlorophenoxyacetic acid + 0.5 mg dm -3 kinetin + 560 mg dm -3 proline + 30 g dm -3 sucrose + 8 g dm -3 agar. Using OsglyII gene, out of 3180 calli bombarded, 32 plants were regenerated on medium containing hygromycin (30 mg dm -3 ). Histochemical GUS assay of the hygromycin selected calli revealed GUS expression in 50 % calli. Among the regenerants, 46.87 % were GUS positive. PCR analysis confirmed the presence of the transgene of 1 kb in 60 % of independent plants. Further, these plants have been grown to maturity in glasshouse. In vitro screening for salt tolerance showed increase in fresh mass of OsglyII putative transgenic calli (185.4 mg) as compared to control calli (84.2 mg) on 90 mM NaCl after 15 d. When exposed to 150 mM NaCl, OsglyII putative transgenic plantlets showed normal growth while the non-transgenic control plantlets turned yellow and finally did not survive.

Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of in vitro raised plantlets.

Abstract: A micropropagation method is described for guava (Psidium guajava L.) using nodal explants from somatic embryo-derived young and aseptic plantlets. Multiple shoots were induced from axillary buds on MS medium containing different concentrations of N 6 -benzylaminopurine (BAP), either alone or in combination with kinetin (Kn), indole-3-acetic acid (IAA) or α-naphthalene acetic acid (NAA). Medium containing 1 mg 1 -1 BAP was the most effective for shoot multiplication. In vitro regenerated shoots developed roots either on MS medium alone or on MS medium supplemented with indole-3-butyric acid (IBA). The rooted plantlets were successfully acclimatized.

High-frequency plantlet regeneration and multiple shoot induction from cultured immature seeds of Rhynchostylis retusa Blume., an exquisite orchid

Abstract: Seeds of an exquisite orchid, Rhynchostylis retusa, germinated in vitro on ½ Murashige and Skoog (MS) medium supplemented with different concentrations of coconut milk (CM). Of the different concentrations of CM employed for seed germination, 15% gave optimum response. On this medium a maximum of 93% cultures produced seedlings 90 days after inoculation. Individual seedlings with a length of about 0.5 cm were subcultured on MS medium supplemented with various concentrations of 6-benzylaminopurine (BA) and α-naphthalene acetic acid (NAA), with or without activated charcoal (AC), for further growth. Seedling growth was maximum on MS medium supplemented with 6 μM BA, 0.2 μM NAA, and 1 g L−1 AC. Here a maximum seedling length of 2.3 cm was observed after 1 month of culture. The seedlings were subcultured on MS medium supplemented with kinetin (Kn) or thidiazuron (TDZ), in the presence or absence of AC, for multiple shoot induction. A maximum multiple shoot number of 8.2 was observed on MS medium supplemented with 2 μM TDZ in the presence of AC. The shoots were rooted on ½ MS medium supplemented with 2 μM indole-3-butyric acid (IBA) and successfully transplanted to soil. Of the 45 plantlets transferred to soil 40 survived. The reproducible protocol standardized here will enable rapid propagation and conservation of this precious orchid.