Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

Nitrate Accumulation, Growth, Yield and chemical composition of Rocket (Eruca vesicaria subsp. Sativa) plant as affected by NPK fertilization, Kinetin and Salicylic Acid

Abstract: In two field experiments, rocket (Eruca vesicaria subsp. sativa) plants were fertilized with low or high nitrogen level as the main plot. Sub-main treatments included; control, double phosphorus (2P), double potassium (2K) and 2K+2P as soil fertilization as well as kinetin (25 and 50 ppm) and salicylic acid (50 and 100 ppm) as foliar application. High level of nitrogen fertilization increased the fresh yield of rocket leaves by 50.0 and 16.4% in the 1st and 2nd cuts, respectively, while these increases in dry yield were 31.6 and 16.8%, respectively as compared to their low nitrogen levels control values. Sub-main treatments of 2P, 2K, 2P+2K, kinetin (25 and 50 ppm) and salicylic acid (50 and 100 ppm) increased both fresh and dry yield of rocket leaves in both 1st and 2nd cuts. The highest percentage increase in total fresh and dry yield were obtained from the treatment with kinetin at the rates of 25 and 50 ppm. Nitrate concentration in the leaf petioles of rocket plant was significantly increased by 41.2 and 75.0% in both 1st and 2nd cut, respectively when fertilized with the high level of nitrogen, while all sub-main treatments significantly decreased it in the 1st cut. Whereas the treatments kinetin (25 and 50 ppm) and salicylic acid (50 ppm) significantly decreased nitrate concentration in the 2nd cut. High nitrogen fertilization increased total nitrogen concentration in the leaves of rocket plant in both cuts. All sub-main treatments, increased total nitrogen concentration in the in the leaves of rocket plants in the 2nd cut. Phosphorus concentration in the leaves of rocket plants decreased as a result of high nitrogen fertilization in both cuts. Treatments of 2P, 2K, 2P+2K and kinetin at 25 ppm increased phosphorus concentration in the leaves of rocket plants in both cuts, while salicylic acid treatments decreased it only in the 2nd cut. Potassium concentration was slightly increased when plants were fertilized with the high nitrogen level, while the treatments kinetin (25 and 50 ppm) and salicylic acid (50 and 100 ppm) slightly resulted in decreasing it in the 1st cut. High nitrogen level fertilization and kinetin (50 ppm) slightly increased iron concentrations in both 1st and 2nd cut. Low values for both manganese and zinc concentrations were observed due to high nitrogen fertilization in the 2nd cut, while salicylic acid treatments at 50 and 100 ppm increased their concentrations, specially in the 2nd cut. Soluble sugars concentrations (reducing, non-reducing and total) in the leaves of rocket plant were decreased when fertilized with the high level of nitrogen, while kinetin at 50 ppm showed the highest increase in these components in both 1st and 2nd cuts. Total free amino acids were increased with the high level of nitrogen fertilization, while all sub-main treatments, except for salicylic acid in the 1st cut and 2P in the 2nd cut, decreased it. High nitrogen fertilization decreased soluble phenols in both 1st and 2nd cut, while the sub-main treatments, kinetin (25 and 50 ppm) and salicylic acid (50 and 100 ppm) increased it by 6.9, 6.9, 18.1 and 8.1%, respectively in the 1st cut. No differences were observed in soluble phenols concentrations in the 2nd cut, except for the treatment salicylic acid at 100 ppm where it was decreased by 28.0%.

Multiplication of Curcuma Species by Tissue Culture

Abstract: Callus induction starting from the rhizome tissue of plants of three CURCUMA species: CURCUMA ZEDOARIA Roscoe, C. DOMESTICA Valeton, and C. AROMATICA Salisbury (Zingiberaceae) was performed. Successful callus formation was obtained in all three species using Murashige-Skoog's (MS) medium supplemented with 1-naphthalene-acetic acid (NAA) (1 ppm) and kinetin (0.1 ppm). Plantlet formation of C. ZEDOARIA from stem tips was successfully conducted on MS medium containing NAA (0-1 ppm) or 6-benzyladenine (BA)(0-3 ppm) at 26 degrees C under the condition of 12 h photoperiod. Production of essential oil in tissue-cultured plants of C. ZEDOARIA were examined using gas chromatography and the same gas chromatogram patterns of essential oil as in the mother plants were detected.

Somatic embryogenesis and plant regeneration of squash Cucurbita pepo L cv. YC 60

Abstract: Plant regeneration from tissue cultures of summer squash, Cucurbita pepo L., cv. YC60, has been observed. Somatic embryos organized from shoot apex derived callus cultured on Murashige and Skoog (MS) medium supplemented with 1.2 mg/l 2,4,5-trichlorophenoxyacetic acid, 0.8 mg/l benzylaminopurine, and 0.1 mg/l kinetin. Embryos developed into plantlets by transfer of immature somatic embryos to MS medium with 0.05 mg/l NAA and 0.05 mg/l kinetin. Regenerated plants appeared morphologically normal and set fruits with seeds which could germinate normally.

In Vitro Regeneration of Stevia rebaudiana (Bert) from the Nodal Explant

Abstract: Procedure for micropropagation of Stevia rebaudiana Bertoni, containing stevioside, a natural noncaloric sweetner, has been developed using nodal segments as explant. Higher proliferation of shoots and multiplication was obtained on Murashige and Skoog basal medium (MS) supplemented with 1.0 mg l-1 indoleacetic acid (IAA) plus 10.0 mg l-1 kinetin and 30.0 mg l-1 adenine sulphate. Sprouting of 90% of the axillary buds was observed within 4 weeks of inoculation, producing >10.0 shoots per explant within 12 weeks. Profuse roots were induced from 90% of the regenerated shoots within 4 weeks of inoculation on half strength MS solid medium supplemented with 1.0 mg l-1 IAA. High survival rate, > 60%, was obtained when the plantlets were transferred to field conditions. The survival rate of taller plants was always higher. The in vitro regenerated plants were morphologically indistinguishable from the donor plants and leaves were of intense sweet taste upon chewing. The heterogenic nature of S. rebaudiana necessitates establishment of protocol for every genotype independently.