Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.)

Abstract: Callus was induced from juice vesicles of satsuma mandarin on Murashige & Skoog medium supplemented with α-naphthaleneacetic acid (NAA), kinetin (K) and gibberellin (GA). Adventitious embryoids arose from the callus tissue on the medium containing 1 mgl−1 NAA alone. The embryoids grew into embryos which resulted in a plantlet on medium containing 1 mgl−1 GA.

Interaction of Kinetin and Calcium in Relation to Their Effect on Stimulation of Ethylene Production

Abstract: Application of kinetin and Ca2+ caused a striking synergistic increase in ethylene production by mung bean (Phaseolus aureus Roxb) hypocotyl segments. The effect of kinetin on Ca2+ uptake and of Ca2+ on the uptake and metabolism of kinetin in relation to their effect on ethylene production was studied. Tracer experiments showed that kinetin greatly increased the uptake of 45Ca2+ after 6 hours of incubation. Reciprocally, Ca2+ stimulated the uptake of kinetin-8-14C and remarkably enhanced the metabolism of kinetin-8-14C into several polar metabolites. Consequently, the quantity of free kinetin-8-14C remaining in Ca2+-treated segments was much less than in control segments. A possible mechanism accounting for the synergism between kinetin and calcium on ethylene production is discussed.

Comparative studies of in vitro and in vivo grown plants and callus of Stevia rebaudiana (Bertoni).

Abstract: Stevia rebaudiana (Bertoni) is investigated as an option for artificial sweetening agents. An efficient plant regeneration via shoot and callus organogenesis was established. Explants were cultured on MS medium containing different concentrations of cytokinins and auxins. Optimal shoot initiation was achieved on medium containing 0.3 mg/l kinetin. Root induction was optimized on MS medium with 2.0 mg/l IBA. Callus optimization was observed on MS medium supplemented with 0.1mg/l 2,4-D.Shoot initiation from callus was maximum in MS+BA +2,4-D and rooting was supported by presence of IBA in MS medium. Rooted plantlets were successfully acclimatized, with a survival rate of 40%.Plantlets and calli grown on MS medium with different plant growth regulators were screened for proximate analysis. Plant and callus regenerated from shoot explant on MS+2,4-D+IBA was proved to be a substantial source for stevioside production. In histological analysis vascular tissue development was seen.

Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers

Abstract: Protocol was developed for high frequency in vitro multiplication of an endemic species, Zingiber rubens Roxb. The sprouted buds of the rhizomes were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA; 0.5–5.0 mg dm−3), indole-3-acetic acid (IAA; 0.5–2.0 mg dm−3), kinetin (KIN; 1.0–3.0 mg dm−3), naphthaleneacetic acid (NAA; 0.5–1.0 mg dm−3) and adenine sulphate (ADS; 80–100 mg dm−3). MS basal medium supplemented with 3 mg dm−3 BA and 0.5 mg dm−3 IAA was optimum for shoot elongation. The elongated shoots (1–2 cm) were transferred to multiplication medium containing 2 mg dm−3 BA, 1 mg dm−3 IAA and 100 mg dm−3 ADS. The multiplication rate remained unchanged in subsequent subcultures. Upon ex vitro transfer, 85 % of plants survived. Genetic stability of micropropagated clones were periodically evaluated at an interval of 6 months up to 30 months in culture using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis and genetic uniformity in all regenerants was confirmed.