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Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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113 citations
113 citations
TL;DR: Kinetin was found to be more effective for shoot regeneration compared with BA, while the highest shoot regeneration frequencies were observed when either cytokinin was combined with high concentration (2.0 mg l−1) of NAA and the optimal concentration of kinetin (67∼77%) was different among the cultivars tested.
Abstract: Optimization of the conditions for an efficient induction of somatic embryogenic calli and regeneration of plants from mature seeds of japonica rice cultivars was attempted The number, color, size, shape, and appearance time of the induced embryogenic calli varied among the rice cultivars depending on the type of basal medium (LS, MS, N6) Presence of adequate amount of sucrose in the medium was an absolute requirement for embryogenic callus formation and shoot induction Induction of the embryogenic calli, whose overall rates ranged from 30 to 56%, was most efficient in N6 medium supplemented with 30 mg l−1 of 2,4-D and 30 g l−1 of sucrose Agar concentration in the regeneration medium was also critical for the shoot induction Kinetin was found to be more effective for shoot regeneration compared with BA, while the highest shoot regeneration frequencies were observed when either cytokinin was combined with high concentration (20 mg l−1) of NAA The optimal concentration of kinetin for the highest shoot regeneration frequency (67∼77%) was different among the cultivars tested The embryogenic calli-derived shoots rooted on a plant growth regulator-free MS medium were successfully established in soil, producing fertile seeds
111 citations
TL;DR: It is suggested that in the evolution of Cuscuta from autotrophic ancestors, the loss of both cotyledons and roots was a single embryonic event and so complete that even under the influence of a highly modified biochemical environment it would be impossible to derive roots from dodder tissue.
Abstract: More than 100 primary root segments from dormant seeds, 100 radicular segments from immature embryos, 450 whole immature embryos, and a number of whole seedlings of C. gronovii were studied under in vitro cultivation in the presence of such growth-regulating factors as: coconut milk, casein hydrolysate, auxilns, kinetin, adenine, gibberellic acid, arnd additional B vitamins as supplements to White's medium. In normal embryogeny early swelling of the basal pole of the embryo and profuse production of epidermal hairs upon germination are superficial characteristics of Cuscuta which resemble seedling root growth in other plants. At no time is there evidence of a primary-root meristem, nor at any time do cotyledons develop. It was not possible to induce root formation experimentally from any of the starting embryonic materials, nor from stems which were cultured to maturity culminating in the production of flowers after 6 months of in vitro cultivation, nor from callus derived from the radicular pole of the embryo. The undifferentiated radicular pole frequently gave rise to rapidly growing callus from which new shoots were formed spontaneously, although sorre immature embryos completed normal embryogeny. Embryo size and subsequent type of development were not strictly correlated but there was a tendency toward the following patterns. Embryos less than 0.4 mm in length lost their original form, callused evenly throughout and became masked by development of buds over the entire surface. Embryos 0.4-1.2 mm were most apt to retain their original form; callus developed at the radicular pole but not at the shoot pole. Embryos 1.5 mm and larger frequently gave rise to radicular callus but also grew normal shoots. Embryo inoculants which had begun to coil were most apt to complete normal embryogenesis. No correlation could be drawn between the addition of specific growth regulators or combiniations of regulators and specific patterns of development. It is suggested that in the evolution of Cuscuta from autotrophic ancestors, the loss of both cotyledons and roots was a single embryonic event and so complete that even under the influence of a highly modified biochemical environment it would be impossible to derive roots from dodder tissue. The usual interpretation of the dodder haustorium as a highly modified adventitious root in all probability is not valid. Is is further suggested, however, that this loss of root and cotyledon is not a necessary consequence of the evolution of the parasitic mode of nutrition, nor of the gross aduilt growth form. THE PURPOSE of this paper is to report the results of experiments designed to test the developmental potential of the ephemeral primary root of Cuscuta gronovii Willd. While different in purpose, 1 Received for publication August 16, 1965. Ths study was completed with the assistance of a grant from the National Science Foundation (G-16436). this investigation yielded certain results which are consistent for the most part with findings of Maheshwari and Baldev (1962), using embryos of C. reflexa which were published after most of the present investigation had been completed. The mature seed of C. gronovii contains a highly coiled embryo devoid of appendages except for a This content downloaded from 207.46.13.38 on Sat, 21 May 2016 05:23:57 UTC All use subject to http://about.jstor.org/terms
111 citations
TL;DR: Multiple shoots were obtained from terminal buds of 20-year-old trees of Eucalyptus citriodora Hook on Murashige and Skoog's medium supplemented with calcium pantothenate, biotin, benzylaminopurine and kinetin.
111 citations